Ruben K Chailakhyan1,2, Elizaveta Kon3,4,5, Anatoly B Shekhter6, Sergey V Ivannikov7, Vladimir I Telpukhov8,9, Alla G Grosheva1, Dmitry S Suslin10, Nataliya N Vorobieva2, Yury V Gerasimov1, Semyon N Churbanov2,11, Svetlana Kotova11,12, Alexey L Fayzullin6, Alexey V Lychagin7,13, Marina M Lipina7,13, Peter S Timashev2,6,10,13,14. 1. N.F.Gamaleya National Research Center for Epidemiology & Microbiology of the Ministry of Health of the Russian Federation, 18 Gamaleya St, Moscow, 123098, Russia. 2. Institute of Photonic Technologies, Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, 2 Pionerskaya St., Moscow, Troitsk, 108840, Russia. 3. Department of Traumatology, Orthopedics and Disaster Surgery, Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya St, 119991, Moscow, Russia. elizaveta.kon@humanitas.it. 4. Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, , Milan, Pieve Emanuele, Italy. elizaveta.kon@humanitas.it. 5. Humanitas Clinical and Research Center - IRCCS, via Manzoni 56, 20089, Milan, Rozzano, Italy. elizaveta.kon@humanitas.it. 6. Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia. 7. Department of Traumatology, Orthopedics and Disaster Surgery, Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya St, 119991, Moscow, Russia. 8. Laboratory of Regeneration of Skeletal Tissues, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia. 9. Department of Topographical Anatomy and Operative Surgery, Sechenov First Moscow State Medical University (Sechenov University), 15\13 Rossolimo St, Moscow, 119991, Russia. 10. Traumatology Department, Clinical and Diagnostic Center "Medsi, Gruzinskiy Per., 3 A. , Moscow, 123056, Russia. 11. Laboratory of Advanced Biomaterials, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia. 12. Department of Polymers and Composites, N.N. Semenov Institute of Chemical Physics, 4 Kosygin St, Moscow, 119991, Russia. 13. Laboratory of Clinical Smart Nanotechnologies, Institute for Regenerative Medicine, Sechenov University, Moscow, Russia. 14. Chemistry Department, Lomonosov Moscow State University, Leninskiye Gory 1‑3, Moscow, 119991, Russia.
Abstract
PURPOSE: To ascertain the role of autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) in the tendon regeneration. METHODS: The study was conducted on 58 Achilles tendons from 29 laboratory Chinchilla adult rabbits. The central bundles of 48 tendons were partially removed and substituted with a tissue-engineered construct consisting of a collagen sponge either loaded with BM-MSCs (n = 24) or cell free (n = 24), placed inside a Vicryl mesh tube. The ends of the resected tendon were inserted in the construct to reach a direct contact with the sponge and sutured to the tube. The animals were sacrificed three and six months post-surgery. Ten intact tendons from five rabbits were used as an untreated control. The tissue samples (n = 30) were stained with haematoxylin and eosin, Picrosirius red, primary antibodies to collagen types I and III and studied by bright-field, phase-contrast, polarized light, and scanning electron microscopies followed by semi-quantitative morphometry. RESULTS: Six months results of cell-loaded scaffolds demonstrated parallel collagen fibres, spindle-shaped tenocytes, and neoangiogenesis. In the control cell-free group, the injured areas were filled with a nonspecific fibrotic tissue with minor foci of incomplete regeneration. The biomechanical tests of 28 tendons taken from 14 rabbits showed that the stiffness of the cell-based reconstructed tendons increased to 98% of the value for the intact samples. CONCLUSION: The obtained results support the hypothesis that the application of BM-MSCs in a tissue-engineered tendon construct leads to the restitution of the tendon tissue.
PURPOSE: To ascertain the role of autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) in the tendon regeneration. METHODS: The study was conducted on 58 Achilles tendons from 29 laboratory Chinchilla adult rabbits. The central bundles of 48 tendons were partially removed and substituted with a tissue-engineered construct consisting of a collagen sponge either loaded with BM-MSCs (n = 24) or cell free (n = 24), placed inside a Vicryl mesh tube. The ends of the resected tendon were inserted in the construct to reach a direct contact with the sponge and sutured to the tube. The animals were sacrificed three and six months post-surgery. Ten intact tendons from five rabbits were used as an untreated control. The tissue samples (n = 30) were stained with haematoxylin and eosin, Picrosirius red, primary antibodies to collagen types I and III and studied by bright-field, phase-contrast, polarized light, and scanning electron microscopies followed by semi-quantitative morphometry. RESULTS: Six months results of cell-loaded scaffolds demonstrated parallel collagen fibres, spindle-shaped tenocytes, and neoangiogenesis. In the control cell-free group, the injured areas were filled with a nonspecific fibrotic tissue with minor foci of incomplete regeneration. The biomechanical tests of 28 tendons taken from 14 rabbits showed that the stiffness of the cell-based reconstructed tendons increased to 98% of the value for the intact samples. CONCLUSION: The obtained results support the hypothesis that the application of BM-MSCs in a tissue-engineered tendon construct leads to the restitution of the tendon tissue.
Authors: Junying Yu; Maxim A Vodyanik; Kim Smuga-Otto; Jessica Antosiewicz-Bourget; Jennifer L Frane; Shulan Tian; Jeff Nie; Gudrun A Jonsdottir; Victor Ruotti; Ron Stewart; Igor I Slukvin; James A Thomson Journal: Science Date: 2007-11-20 Impact factor: 47.728
Authors: Luciano C Ramires; Madhan Jeyaraman; Sathish Muthu; Navaladi Shankar A; Gabriel Silva Santos; Lucas Furtado da Fonseca; José Fábio Lana; Ramya Lakshmi Rajendran; Prakash Gangadaran; Manasi P Jogalekar; Alfredo A Cardoso; Alex Eickhoff Journal: Life (Basel) Date: 2022-03-09