| Literature DB >> 34495504 |
Takashi Kiuchi1, Susumu Katsuma2.
Abstract
The molecular mechanisms of sex-determination systems among insect orders and species are diverse. Therefore, genes involved in sex determination are strong candidates for insect pest management. Even though lepidopterans are major agricultural insect pests that cause widespread economic damage to various crops, their sex-determination systems have not been fully elucidated, even in the silkworm (Bombyx mori), a model lepidopteran insect. In 2014, we found that a female-specific W chromosome-derived PIWI-interacting RNA (piRNA) determines femaleness in silkworms. To analyze the function of two core silkworm piRNA biogenesis pathway genes, Siwi and BmAgo3, in the sex-determination system, we developed a genomic DNA and total RNA extraction strategy for a siRNA-injected single embryo. The siRNA-injected embryo can be molecularly sexed by W chromosome-specific DNA markers. Using complementary DNA (cDNA) reverse transcribed from the sexed RNA, we evaluated the knockdown effect of the PIWI protein-coding genes on a sexual development-related gene, Bombyx mori doublesex.Entities:
Keywords: BmAgo3; Bombyx mori; Bombyx mori doublesex; Embryonic RNAi; PIWI-interacting RNAs; Sex determination; Siwi
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Year: 2022 PMID： 34495504 DOI： 10.1007/978-1-0716-1633-8_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745