Samia Zrelli1,2, Safa Amairia3, Moez Mhadhbi3, Oussama Briki4, Seifeddine Bouallegue5, Fatma Souissi6, Mohamed Gharbi3. 1. Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia. samiazrelli@yahoo.fr. 2. Laboratoire de Microbiologie Alimentaire, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia. samiazrelli@yahoo.fr. 3. Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia. 4. Service contrôle qualité, abattoir El Mazraa, Poulina Group Holding, Km 25 Fondek Jedid, 8012, Grombalia, Tunisia. 5. Service contrôle qualité, abattoir Dick, Poulina Group Holding, Km 25 Fondek Jedid, 8012, Grombalia, Tunisia. 6. Laboratoire de Microbiologie Alimentaire, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia.
Abstract
PURPOSE: Food-borne Toxoplasma gondii infection is ranked among parasitic diseases of global concern. Toxoplasmosis is qualified as a one health disease, since it affects human health, animals, and ecosystems. The present study was performed to estimate the molecular prevalence of T. gondii in industrial poultry meat samples from Tunisia. A total of 140 breast samples were collected from chicken (Gallus gallus domesticus) and turkey (Meleagris gallopavo) and four breeding systems (broiler chickens, reform laying hens, chicken farm label, and broiler turkeys) cover the majority of chicken meat production in Tunisia. METHODS: For each sample, DNA was extracted and T. gondii-specific PCR, targeting the B1 gene was performed. RESULTS: The overall molecular prevalence of T. gondii in poultry meat samples was 20% (28/140; 95% CI: 13.3-26.6%). The highest prevalence was observed in chicken farm label (25.7%; 9/35). Prevalence rates were 20.0% (7/35), 20.0% (7/35), and 14.3% (5/35) in broiler chickens, broiler turkeys, and reform laying hens, respectively (p > 0.05). CONCLUSION: Our finding suggests the involvement of industrial chicken meat, as a potential risk factor of human T. gondii transmission, especially when the latter is consumed undercooked.
PURPOSE: Food-borne Toxoplasma gondii infection is ranked among parasitic diseases of global concern. Toxoplasmosis is qualified as a one health disease, since it affects human health, animals, and ecosystems. The present study was performed to estimate the molecular prevalence of T. gondii in industrial poultry meat samples from Tunisia. A total of 140 breast samples were collected from chicken (Gallus gallus domesticus) and turkey (Meleagris gallopavo) and four breeding systems (broiler chickens, reform laying hens, chicken farm label, and broiler turkeys) cover the majority of chicken meat production in Tunisia. METHODS: For each sample, DNA was extracted and T. gondii-specific PCR, targeting the B1 gene was performed. RESULTS: The overall molecular prevalence of T. gondii in poultry meat samples was 20% (28/140; 95% CI: 13.3-26.6%). The highest prevalence was observed in chicken farm label (25.7%; 9/35). Prevalence rates were 20.0% (7/35), 20.0% (7/35), and 14.3% (5/35) in broiler chickens, broiler turkeys, and reform laying hens, respectively (p > 0.05). CONCLUSION: Our finding suggests the involvement of industrial chicken meat, as a potential risk factor of human T. gondii transmission, especially when the latter is consumed undercooked.
Authors: J P Dubey; D E Hill; J L Jones; A W Hightower; E Kirkland; J M Roberts; P L Marcet; T Lehmann; M C B Vianna; K Miska; C Sreekumar; O C H Kwok; S K Shen; H R Gamble Journal: J Parasitol Date: 2005-10 Impact factor: 1.276
Authors: G Schares; B Bangoura; F Randau; T Goroll; M Ludewig; P Maksimov; B Matzkeit; M Sens; A Bärwald; F J Conraths; M Opsteegh; J Van der Giessen Journal: Int J Parasitol Date: 2017-09-05 Impact factor: 3.981
Authors: Fernando Jorge Rodrigues Magalhães; José Givanildo da Silva; Müller Ribeiro-Andrade; José Wilton Pinheiro; Rinaldo Aparecido Mota Journal: Acta Trop Date: 2016-03-28 Impact factor: 3.112