| Literature DB >> 34494426 |
Chuanyu Wang1, Chung-Hui Huang2, Zhuangqiang Gao1, Jialiang Shen1, Jiacheng He1, Alana MacLachlan1, Chao Ma3,4, Ya Chang2, Wen Yang1, Yuxin Cai1, Yang Lou5, Siyuan Dai1, Weiqiang Chen3,4, Feng Li2, Pengyu Chen1.
Abstract
Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis of exosomes and exosome-shuttled proteins would be of immense value in understanding cancer progression and generating reliable predictive biomarkers for cancer diagnosis and treatment. Recent studies have indicated the critical role of exosomal programmed death ligand 1 (PD-L1) in immune checkpoint therapy and its application as a patient stratification biomarker in cancer immunotherapy. Here, we present a nanoplasmonic exosome immunoassay utilizing gold-silver (Au@Ag) core-shell nanobipyramids and gold nanorods, which form sandwich immune complexes with target exosomes. The immunoassay generates a distinct plasmonic signal pattern unique to exosomes with specific exosomal PD-L1 expression, allowing rapid, highly sensitive exosome detection and accurate identification of PD-L1 exosome subtypes in a single assay. The developed nanoplasmonic sandwich immunoassay provides a novel and viable approach for tumor cell-derived exosome detection and analysis with quantitative molecular details of key exosomal proteins, manifesting its great potential as a transformative diagnostic tool for early cancer detection, prognosis, and post-treatment monitoring.Entities:
Keywords: exosomal protein; exosomes; gold−silver core−shell nanobipyramids (Au@Ag NBPs); immunoassay; localized surface plasmon resonance (LSPR); microfluidics; programmed death ligand 1 (PD-L1)
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Year: 2021 PMID： 34494426 PMCID： PMC9275046 DOI： 10.1021/acssensors.1c01101
Source DB: PubMed Journal: ACS Sens ISSN： 2379-3694 Impact factor: 9.618