Lourdes Alvarez-Arellano1, Pilar Eguía-Aguilar2, Patricia Piña-Sánchez3, Nadia González-García4, Alam Palma-Guzman5, Mario Perezpeña-Diazconti6, Carmen Maldonado-Bernal7. 1. CONACYT-Hospital Infantil de México Federico Gómez, Mexico City, Mexico. 2. Laboratorio de Biología Molecular, Departamento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, Mexico City, Mexico. 3. Laboratorio de Oncología Molecular, Unidad de Investigación Médica en Enfermedades Oncológicas, Instituto Mexicano del Seguro Social, Mexico City, Mexico. 4. Laboratorio de Neurociencias, Hospital Infantil de México Federico Gómez, Mexico City, Mexico. 5. Laboratorio Nacional de Microscopia Avanzada, Instituto Mexicano del Seguro Social, Mexico City, Mexico. 6. Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico. 7. Unidad de Investigación en Inmunología y Proteómica , Hospital Infantil de México Federico Gómez, Mexico City, Mexico. cmaldobe@yahoo.com.
Abstract
PURPOSE: Medulloblastoma is an embryonal brain tumor that predominantly occurs in childhood with a wide histological and molecular variability. Our aim was to investigate the expression of Toll-like receptors (TLRs), their association with the infiltration of immune cells and with the histological subgroups, and, also, with the overall survival of patients. METHODS: Fifty-six paraffin-preserved biopsies from children with medulloblastoma of the classic, desmoplastic, and anaplastic subtypes were included. Microarrays of tissues were performed, and the infiltration of T and NK cells was quantified, as well as the expression of TLR7, TLR8, and TLR9. For all statistical analyses, significance was p < 0.05. RESULTS: CD4 + and CD8 + T lymphocytes and NK cells were found infiltrating the tumor. The infiltration of NK and CD4 + cells was greater in the classic and desmoplastic subtypes than in anaplastic. We found an important expression of TLRs in all medulloblastomas, but TLR7 and TLR8 were considerably higher in classic and desmoplastic subtypes than in anaplastic. Importantly, we observed that TLR7 was a prognostic factor for survival. CONCLUSIONS: Medulloblastomas present cellular infiltration and a differential expression of TLRs depending on the histological subtype. TLR7 is a prognostic factor of survival that is dependent on treatment and age.
PURPOSE: Medulloblastoma is an embryonal brain tumor that predominantly occurs in childhood with a wide histological and molecular variability. Our aim was to investigate the expression of Toll-like receptors (TLRs), their association with the infiltration of immune cells and with the histological subgroups, and, also, with the overall survival of patients. METHODS: Fifty-six paraffin-preserved biopsies from children with medulloblastoma of the classic, desmoplastic, and anaplastic subtypes were included. Microarrays of tissues were performed, and the infiltration of T and NK cells was quantified, as well as the expression of TLR7, TLR8, and TLR9. For all statistical analyses, significance was p < 0.05. RESULTS: CD4 + and CD8 + T lymphocytes and NK cells were found infiltrating the tumor. The infiltration of NK and CD4 + cells was greater in the classic and desmoplastic subtypes than in anaplastic. We found an important expression of TLRs in all medulloblastomas, but TLR7 and TLR8 were considerably higher in classic and desmoplastic subtypes than in anaplastic. Importantly, we observed that TLR7 was a prognostic factor for survival. CONCLUSIONS: Medulloblastomas present cellular infiltration and a differential expression of TLRs depending on the histological subtype. TLR7 is a prognostic factor of survival that is dependent on treatment and age.
Authors: Charles G Eberhart; James L Kepner; Patricia T Goldthwaite; Larry E Kun; Patricia K Duffner; Henry S Friedman; Douglas R Strother; Peter C Burger Journal: Cancer Date: 2002-01-15 Impact factor: 6.860
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Authors: Paul Gibson; Yiai Tong; Giles Robinson; Margaret C Thompson; D Spencer Currle; Christopher Eden; Tanya A Kranenburg; Twala Hogg; Helen Poppleton; Julie Martin; David Finkelstein; Stanley Pounds; Aaron Weiss; Zoltan Patay; Matthew Scoggins; Robert Ogg; Yanxin Pei; Zeng-Jie Yang; Sonja Brun; Youngsoo Lee; Frederique Zindy; Janet C Lindsey; Makoto M Taketo; Frederick A Boop; Robert A Sanford; Amar Gajjar; Steven C Clifford; Martine F Roussel; Peter J McKinnon; David H Gutmann; David W Ellison; Robert Wechsler-Reya; Richard J Gilbertson Journal: Nature Date: 2010-12-08 Impact factor: 49.962