Ali Rajabi1, Sepideh Bastani2, Melika Maydanchi3, Samaneh Tayefeh-Gholami1, Sepehr Abdolahi4, Ali Saber3, Reza Safaralizadeh5. 1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. 2. Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran. 3. Zimagene Medical Genetics Laboratory, Avicenna St., Hamedan, Iran. 4. Department of Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. 5. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Safaralizadeh@tabrizu.ac.ir.
Abstract
PURPOSE: Gastric cancer (GC) is one of the most frequent tumors worldwide and identification of a sensitive and specific prognostic biomarker is of great importance. Long non-coding RNAs (lncRNAs) play crucial roles in tumorigenesis of various malignancies. In the present study, we investigated lncRNA FOXD2-AS1 expression in gastric tumors and assessed its potential as a prognostic biomarker. METHODS: A total of 95 tumor and corresponding adjacent non-tumor tissue specimens were collected from patients with GC from Imam Reza hospital, Tabriz, Iran. Total RNA was isolated and FOXD2-AS1 expression was measured using quantitative reverse transcriptase (qRT)-PCR. RESULTS: FOXD2-AS1 was significantly upregulated in tumor samples as compared to non-tumor tissues (P < 0.0001). In addition, higher expression of FOXD2-AS1 was significantly associated with lymph node metastasis and Helicobacter pylori infection. The receiver operating characteristic (ROC) curve analysis revealed that FOXD2-AS1 might be served as a potential prognostic biomarker for GC. CONCLUSION: FOXD2-AS1 is upregulated in gastric tumors and can be used as a valuable biomarker in the prognosis of patients with GC.
PURPOSE: Gastric cancer (GC) is one of the most frequent tumors worldwide and identification of a sensitive and specific prognostic biomarker is of great importance. Long non-coding RNAs (lncRNAs) play crucial roles in tumorigenesis of various malignancies. In the present study, we investigated lncRNA FOXD2-AS1 expression in gastric tumors and assessed its potential as a prognostic biomarker. METHODS: A total of 95 tumor and corresponding adjacent non-tumor tissue specimens were collected from patients with GC from Imam Reza hospital, Tabriz, Iran. Total RNA was isolated and FOXD2-AS1 expression was measured using quantitative reverse transcriptase (qRT)-PCR. RESULTS: FOXD2-AS1 was significantly upregulated in tumor samples as compared to non-tumor tissues (P < 0.0001). In addition, higher expression of FOXD2-AS1 was significantly associated with lymph node metastasis and Helicobacter pylori infection. The receiver operating characteristic (ROC) curve analysis revealed that FOXD2-AS1 might be served as a potential prognostic biomarker for GC. CONCLUSION: FOXD2-AS1 is upregulated in gastric tumors and can be used as a valuable biomarker in the prognosis of patients with GC.
Authors: Alfred S L Cheng; May S Li; Wei Kang; Victoria Y Cheng; Jian-Liang Chou; Suki S Lau; Minnie Y Go; Ching C Lee; Thomas K Ling; Enders K Ng; Jun Yu; Tim H Huang; Ka F To; Michael W Chan; Joseph J Y Sung; Francis K L Chan Journal: Gastroenterology Date: 2012-10-08 Impact factor: 22.682