Comparison of measurement methods at determining the target sites injured by antimicrobials in Escherichia coli O157:H7 using metabolic inhibitors.

Acquiring an understanding of the mechanisms underlying antimicrobial action is important for overcoming bacterial resistance to antimicrobials. This study evaluated three different methods (antimicrobial fixed broth dilution method, metabolic inhibitors fixed broth dilution method, and metabolic inhibitor fixed agar recovery method) for determining the target site of Escherichia coli O157:H7 by treatments with various antimicrobials (ethanol, ethylenediaminetetraacetic acid, polymyxin B, thymol, acetic acid, and citrus fruit extract). However, the results indicated only weak relationships between MIC values and mechanisms of antimicrobials known to cause damage or injury. In addition, the results of three measurement methods using metabolic inhibitors were not correlated. These results suggest that measurement methods using metabolic inhibitors alone may not be suitable for determining the target site injured by antimicrobials. Therefore, various measurement methods should be compared and analyzed to determine the damage or injury sites targeted by antimicrobials in pathogenic bacteria. Further studies are needed to compare and analyze the various measurement methods for determining the target site injured by antimicrobials in pathogenic bacteria. © The Korean Society of Food Science and Technology 2021.