| Literature DB >> 34467227 |
Yuefeng Li1,2, Kiran Nakka1,2, Thomas Olender1,2, Philippe Gingras-Gelinas3, Matthew Man-Kin Wong1,2, Daniel C L Robinson1,2, Hina Bandukwala1, Carmen G Palii1, Odile Neyret3, Marjorie Brand1,2,4, Alexandre Blais5,6,7, F Jeffrey Dilworth1,2,4,5.
Abstract
Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34+ hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021).Entities:
Keywords: ChIPseq; cell biology; cell isolation; chromatin immunoprecipitation (ChIP); flow cytometry/mass cytometry; molecular biology; sequence analysis; sequencing; stem cells
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Year: 2021 PMID： 34467227 PMCID： PMC8384913 DOI： 10.1016/j.xpro.2021.100751
Source DB: PubMed Journal: STAR Protoc ISSN： 2666-1667