Jhenifer Nascimento da Silva1,2, Daniel Luiz Reis Simas3, Angelica Ribeiro Soares1, Heitor Monteiro Duarte1, Jorge Moraes2, Christiano Calixto Conceição2, Renato Martins da Silva2, Itabajara da Silva Vaz4, Carlos Logullo5,6. 1. Grupo de Produtos Naturais de Organismos Aquáticos, Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, RJ, Brazil. 2. Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil. 3. Fábrica de Árvores Soluções Ambientais, Sitio Anjo Gabriel, Bragança Paulista, São Paulo, SP, Brazil. 4. Centro de Biotecnologia and Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. 5. Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil. carlos.logullo@bioqmed.ufrj.br. 6. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. carlos.logullo@bioqmed.ufrj.br.
Abstract
INTRODUCTION: Metabolomic approaches can assess the actual state of an organism's energy metabolism during a specific morphological event, providing a more accurate insight into the correlations between physiology and metabolic regulation. METHODS: The study of the metabolomic profile aim to identify the largest possible number of biomolecules in a certain organism or specific structures. For this purpose, mass spectrometry (MS) and chromatography have been used in the present study. OBJECTIVES: In this context, the aim of the present work is to evaluate the glucose metabolomic profile during embryogenesis in Rhipicephalus microplus tick, investigating the dynamics of nutrient utilization during tick embryo formation, as well as the control of glucose metabolism. RESULTS: We show that glycogen reserves are preferentially mobilized to sustain the energy-intensive process of embryogenesis. Subsequently, the increase in concentration of specific amino acids indicates that protein degradation would provide carbons to fuel gluconeogenesis, supplying the embryo with sufficient glucose and glycogen during development. CONCLUSION: Altogether, these results demonstrated the presence of a very refined catabolic and anabolic control during embryogenesis in R. microplus tick, suggesting the pronounced gluconeogenesis as a strategy to secure embryo development. Moreover, this research contributes to the understanding of the mechanisms that control glucose metabolism during tick embryogenesis and may aid the identification of putative targets for novel chemical or immunological control methods, which are essential to improve the prevention of tick infestations.
INTRODUCTION: Metabolomic approaches can assess the actual state of an organism's energy metabolism during a specific morphological event, providing a more accurate insight into the correlations between physiology and metabolic regulation. METHODS: The study of the metabolomic profile aim to identify the largest possible number of biomolecules in a certain organism or specific structures. For this purpose, mass spectrometry (MS) and chromatography have been used in the present study. OBJECTIVES: In this context, the aim of the present work is to evaluate the glucose metabolomic profile during embryogenesis in Rhipicephalus microplus tick, investigating the dynamics of nutrient utilization during tick embryo formation, as well as the control of glucose metabolism. RESULTS: We show that glycogen reserves are preferentially mobilized to sustain the energy-intensive process of embryogenesis. Subsequently, the increase in concentration of specific amino acids indicates that protein degradation would provide carbons to fuel gluconeogenesis, supplying the embryo with sufficient glucose and glycogen during development. CONCLUSION: Altogether, these results demonstrated the presence of a very refined catabolic and anabolic control during embryogenesis in R. microplus tick, suggesting the pronounced gluconeogenesis as a strategy to secure embryo development. Moreover, this research contributes to the understanding of the mechanisms that control glucose metabolism during tick embryogenesis and may aid the identification of putative targets for novel chemical or immunological control methods, which are essential to improve the prevention of tick infestations.
Authors: Mark R Brown; Kevin D Clark; Monika Gulia; Zhangwu Zhao; Stephen F Garczynski; Joe W Crim; Richard J Suderman; Michael R Strand Journal: Proc Natl Acad Sci U S A Date: 2008-04-07 Impact factor: 11.205
Authors: Sasha Babicki; David Arndt; Ana Marcu; Yongjie Liang; Jason R Grant; Adam Maciejewski; David S Wishart Journal: Nucleic Acids Res Date: 2016-05-17 Impact factor: 16.971