Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes.

Literature DB >> 3446172

A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes.

Abstract

1. A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide ('MEGA-10') was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins.

Entities: Chemical Species

Mesh：

Substances：

Year: 1987 PMID： 3446172 PMCID： PMC1148024 DOI： 10.1042/bj2440503

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

28 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

2. Quantitative analysis of phospholipids by thin-layer chromatography.

Authors: V P Skipski; R F Peterson; M Barclay
Journal: Biochem J Date: 1964-02 Impact factor: 3.857

3. A rapid method for the preparation of microvilli from rabbit kidney.

Authors: A G Booth; A J Kenny
Journal: Biochem J Date: 1974-09 Impact factor: 3.857

4. Incorporation of D-glucose-, L-alanine- and phosphate-transport systems from rat renal brush-border membranes into liposomes.

Authors: R Kinne; R G Faust
Journal: Biochem J Date: 1977-11-15 Impact factor: 3.857

5. Phenylalanine uptake in isolated renal brush border vesicles.

Authors: J Evers; H Murer; R Kinne
Journal: Biochim Biophys Acta Date: 1976-04-05

6. Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine.

Authors: C Baron; T E Thompson
Journal: Biochim Biophys Acta Date: 1975-03-25

7. Transport of amino acids in renal brush border membrane vesicles. Uptake of L-proline.

Authors: M R Hammerman; B Sacktor
Journal: J Biol Chem Date: 1977-01-25 Impact factor: 5.157

8. Transport of amino acids in renal brush border membrane vesicles. Uptake of the neutral amino acid L-alanine.

Authors: S J Fass; M R Hammerman; B Sacktor
Journal: J Biol Chem Date: 1977-01-25 Impact factor: 5.157

9. A simple and efficient method for reconstitution of amino acid and glucose transport systems from Ehrlich ascites cells.

Authors: J I McCormick; D Tsang; R M Johnstone
Journal: Arch Biochem Biophys Date: 1984-06 Impact factor: 4.013

10. Kinetics of Na+-dependent D-glucose transport.

Authors: U Hopfer
Journal: J Supramol Struct Date: 1977

12 in total

1. Reconstitution of the human placental 5-hydroxytryptamine transporter in a catalytically active form after detergent solubilization.

Authors: S Ramamoorthy; D R Cool; F H Leibach; V B Mahesh; V Ganapathy
Journal: Biochem J Date: 1992-08-15 Impact factor: 3.857

2. Reconstitution of the renal brush-border membrane sodium/phosphate co-transporter.

Authors: V Vachon; M C Delisle; R Laprade; R Béliveau
Journal: Biochem J Date: 1991-09-01 Impact factor: 3.857

3. Reconstitution and identification of the major Na(+)-dependent neutral amino acid-transport protein from bovine renal brush-border membrane vesicles.

Authors: F A Doyle; J D McGivan
Journal: Biochem J Date: 1992-01-01 Impact factor: 3.857

4. Amino acid deprivation leads to the emergence of System A activity and the synthesis of a specific membrane glycoprotein in the bovine renal epithelial cell line NBL-1.

Authors: A Felipe; C Soler; J D McGivan
Journal: Biochem J Date: 1992-06-01 Impact factor: 3.857

5. A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.

Authors: A R Quesada; J D McGivan
Journal: Biochem J Date: 1988-11-01 Impact factor: 3.857