Literature DB >> 3214433

A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.

A R Quesada1, J D McGivan.   

Abstract

A rapid method for the functional reconstruction of amino acid transport from liver plasma-membrane vesicles using the neutral detergent decanoyl-N-glucamide ('MEGA-10') is described. The method is a modification of that previously employed in this laboratory for reconstitution of amino acid transport systems from kidney brush-border membranes [Lynch & McGivan (1987) Biochem. J. 244, 503-508]. The transport activities termed 'System A', 'System N', and 'System L' are all reconstituted. The reconstitution procedure is rapid and efficient and is suitable as an assay for transport activity in studies involving membrane fractionation. By using this reconstitution procedure, System A transport activity was partially purified by lectin-affinity chromatography.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3214433      PMCID： PMC1135335          DOI： 10.1042/bj2550963

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

1.  ON THE MECHANISM OF ACTION OF PHOSPHOLIPASE A.

Authors:  R M DAWSON
Journal:  Biochem J       Date:  1963-09       Impact factor: 3.857

2.  Apparent inhibition of active non-electrolyte transport by an increased sodium permeability of the plasma membrane. Mechanism of action of p-chloromercuribenzene sulfonate.

Authors:  P C Will; U Hopfer
Journal:  J Biol Chem       Date:  1979-05-25       Impact factor: 5.157

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  The role of SH-groups in the concentrative transport of D-glucose into brush border membrane vesicles.

Authors:  J Biber; H Hauser
Journal:  FEBS Lett       Date:  1979-12-15       Impact factor: 4.124

5.  Amino acid transport in plasma membrane vesicles from isolated rat liver parenchymal cells.

Authors:  H J Sips; M E Apitule; K van Dam
Journal:  Biochim Biophys Acta       Date:  1980-08-04

6.  Sodium-dependent alanine transport in plasma-membrane vesicles from rat liver.

Authors:  J M van Amelsvoort; H J Sips; K van Dam
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

7.  Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs.

Authors:  M S Kilberg; M E Handlogten; H N Christensen
Journal:  J Biol Chem       Date:  1980-05-10       Impact factor: 5.157

8.  Reconstitution of the L-lactate carrier from rat and rabbit erythrocyte plasma membranes.

Authors:  R C Poole; A P Halestrap
Journal:  Biochem J       Date:  1988-09-01       Impact factor: 3.857

9.  Amino acid transport in plasma-membrane vesicles from rat liver. Characterization of L-alanine transport.

Authors:  H J Sips; J M Van Amelsvoort; K Van Dam
Journal:  Eur J Biochem       Date:  1980-04

10.  Amino acid-dependent sodium transport in plasma membrane vesicles from rat liver.

Authors:  H J Sips; K van Dam
Journal:  J Membr Biol       Date:  1981       Impact factor: 1.843
View more
  13 in total

1.  Amino acid transport system A resembles system N in sequence but differs in mechanism.

Authors:  R J Reimer; F A Chaudhry; A T Gray; R H Edwards
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-05       Impact factor: 11.205

2.  Reconstitution of the human placental 5-hydroxytryptamine transporter in a catalytically active form after detergent solubilization.

Authors:  S Ramamoorthy; D R Cool; F H Leibach; V B Mahesh; V Ganapathy
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

3.  Functional reconstitution of the hepatic system N amino acid transport activity.

Authors:  B K Tamarappoo; M S Kilberg
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

4.  Reconstitution and identification of the major Na(+)-dependent neutral amino acid-transport protein from bovine renal brush-border membrane vesicles.

Authors:  F A Doyle; J D McGivan
Journal:  Biochem J       Date:  1992-01-01       Impact factor: 3.857

5.  Amino acid deprivation leads to the emergence of System A activity and the synthesis of a specific membrane glycoprotein in the bovine renal epithelial cell line NBL-1.

Authors:  A Felipe; C Soler; J D McGivan
Journal:  Biochem J       Date:  1992-06-01       Impact factor: 3.857

6.  Expression of rat liver Na+/L-alanine co-transport in Xenopus laevis oocytes. Effect of glucagon in vivo.

Authors:  M Palacin; A Werner; J Dittmer; H Murer; J Biber
Journal:  Biochem J       Date:  1990-08-15       Impact factor: 3.857

Review 7.  Regulatory and molecular aspects of mammalian amino acid transport.

Authors:  J D McGivan; M Pastor-Anglada
Journal:  Biochem J       Date:  1994-04-15       Impact factor: 3.857

8.  Sensitivity of system A and ASC transport activities to thiol-group-modifying reagents in rat liver plasma-membrane vesicles. Evidence for a direct binding of N-ethylmaleimide and iodoacetamide on A and ASC carriers.

Authors:  E Pola; J Bertran; A Roca; M Palacín; A Zorzano; X Testar
Journal:  Biochem J       Date:  1990-10-15       Impact factor: 3.857

9.  Evidence for a regulatory protein involved in the increased activity of system A for neutral amino acid transport in osmotically stressed mammalian cells.

Authors:  B Ruiz-Montasell; M Gómez-Angelats; F J Casado; A Felipe; J D McGivan; M Pastor-Anglada
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205

10.  Reconstitution studies of amino acid transport system L in rat erythrocytes.

Authors:  S Y Yao; R George; J D Young
Journal:  Biochem J       Date:  1993-06-15       Impact factor: 3.857
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.