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Literature DB >> 34453707

Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase.

Rachel C Gomez¹, Edmundo G Vides¹, Suzanne R Pfeffer².

Abstract

Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating proteins. Its fast nucleotide exchange rate and inability to bind GDI in cytosol make it a unique and poorly understood Rab. Because the conventional, "GTP-locked" Rab mutation does not have the desired effect in Rab29, we present here the use of a fluorescence-based assay to characterize novel Rab29 mutants (I64T and V156G) that display faster nucleotide exchange rates, allowing for GEF-independent Rab29 activation.

Entities: Chemical

Keywords: Fast exchange mutant; MANT-GDP; Nucleotide exchange; Rab GTPase; Rab29

Mesh：

Substances：

Year: 2021 PMID： 34453707 DOI： 10.1007/978-1-0716-1346-7_2

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

11 in total

Review 1. GEFs and GAPs: critical elements in the control of small G proteins.

Authors: Johannes L Bos; Holger Rehmann; Alfred Wittinghofer
Journal: Cell Date: 2007-06-01 Impact factor: 41.582

2. Catalytic mechanism of a mammalian Rab·RabGAP complex in atomic detail.

Authors: Konstantin Gavriljuk; Emerich-Mihai Gazdag; Aymelt Itzen; Carsten Kötting; Roger S Goody; Klaus Gerwert
Journal: Proc Natl Acad Sci U S A Date: 2012-12-12 Impact factor: 11.205

3. Kinetic analysis of the binding of guanine nucleotide to bovine brain smg p25A.

Authors: I Shoji; A Kikuchi; S Kuroda; Y Takai
Journal: Biochem Biophys Res Commun Date: 1989-07-14 Impact factor: 3.575

Review 4. Rab GEFs and GAPs.

Authors: Francis Barr; David G Lambright
Journal: Curr Opin Cell Biol Date: 2010-05-11 Impact factor: 8.382

5. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase.

Authors: Alexey Rak; Olena Pylypenko; Thomas Durek; Anja Watzke; Susanna Kushnir; Lucas Brunsveld; Herbert Waldmann; Roger S Goody; Kirill Alexandrov
Journal: Science Date: 2003-10-24 Impact factor: 47.728

6. The small GTPase Rab29 is a common regulator of immune synapse assembly and ciliogenesis.

Authors: A Onnis; F Finetti; L Patrussi; M Gottardo; C Cassioli; S Spanò; C T Baldari
Journal: Cell Death Differ Date: 2015-03-13 Impact factor: 15.828

7. A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor.

Authors: Shicong Wang; Zexu Ma; Xiaohui Xu; Zhen Wang; Lixiang Sun; Yunhe Zhou; Xiaosi Lin; Wanjin Hong; Tuanlao Wang
Journal: PLoS One Date: 2014-05-02 Impact factor: 3.240

Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase.

Review 1. GEFs and GAPs: critical elements in the control of small G proteins.

2. Catalytic mechanism of a mammalian Rab·RabGAP complex in atomic detail.

3. Kinetic analysis of the binding of guanine nucleotide to bovine brain smg p25A.

Review 4. Rab GEFs and GAPs.

5. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase.

6. The small GTPase Rab29 is a common regulator of immune synapse assembly and ciliogenesis.

7. A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor.

8. Lysosome biogenesis requires Rab9 function and receptor recycling from endosomes to the trans-Golgi network.

9. Rab29 activation of the Parkinson's disease-associated LRRK2 kinase.

10. Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases.

1. A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.