Omid Babania1,2,3, Saeed Mohammadi4,5, Esmat Yaghoubi3, Ahmad Sohrabi4, Fakhri Sadat Seyedhosseini6, Nafiseh Abdolahi7, Yaghoub Yazdani1,5. 1. Department of Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. 2. Amirkola Shafizadeh Pediatric Hospital, Babol University of Medical Sciences, Babol, Iran. 3. Razi Pathobiology and Genetics Laboratory, Babol, Iran. 4. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran. 5. Stem Cell Research center, Golestan University of Medical Sciences, Gorgan, Iran. 6. Department of Internal Medicine, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. 7. Golestan Rheumatology Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Abstract
Celiac disease (CD) is a chronic autoimmune disorder of small intestine against dietary gluten, among genetically predisposed individuals. Monocytes are versatile innate immune cells involved in the regulation of inflammation, and strongly involved in the intestinal immunity. However, the role of monocytes and their subtypes in CD is not well demonstrated. METHODS: Here, we assessed the polarization of CD14+ monocytes by evaluating the M1 (CD16) and M2 (CD163) markers by flowcytometry, their soluble forms (sCD16 and sCD163), and the serum levels of IL-10, IL-12, TGF-β, and TNF-α cytokines using ELISA method, among 30 CD patients and 30 sex- and age-matched healthy subjects (HS). We also analyzed the diagnostic values of all variables with significant differences. RESULTS: CD14+CD163+ monocytes were more frequent in CD patients than HS, while CD14+CD16+ monocytes were higher in HS. IL-10and TNF-α increased, and TGF-β expression was decreased among CD patients. The sCD16 serum levels were elevated in patients, while sCD163 was higher but not significant among CD patients. CD163+/CD16+ and IL-10/IL-12 ratios were higher in CD patients, and TGFβ/TNFα ratio was higher in HS group. IL-10, CD14+CD163+, TNF-α, and IL-10/IL-12 ratios with the AUC over 0.7 were introduced as fair diagnostic markers. Our findings revealed that the M2 (CD14+CD163+) monocytes were more frequent among CD patients, and the cytokine balance was disturbed. CONCLUSION: According to the significant functional diversities of monocyte subtypes between CD patients and HS group, these immunologic markers could be introduced as specific diagnostic biomarkers for CD.
Celiac disease (CD) is a chronic autoimmune disorder of small intestine against dietary gluten, among genetically predisposed individuals. Monocytes are versatile innate immune cells involved in the regulation of inflammation, and strongly involved in the intestinal immunity. However, the role of monocytes and their subtypes in CD is not well demonstrated. METHODS: Here, we assessed the polarization of CD14+ monocytes by evaluating the M1 (CD16) and M2 (CD163) markers by flowcytometry, their soluble forms (sCD16 and sCD163), and the serum levels of IL-10, IL-12, TGF-β, and TNF-α cytokines using ELISA method, among 30 CD patients and 30 sex- and age-matched healthy subjects (HS). We also analyzed the diagnostic values of all variables with significant differences. RESULTS: CD14+CD163+ monocytes were more frequent in CD patients than HS, while CD14+CD16+ monocytes were higher in HS. IL-10and TNF-α increased, and TGF-β expression was decreased among CD patients. The sCD16 serum levels were elevated in patients, while sCD163 was higher but not significant among CD patients. CD163+/CD16+ and IL-10/IL-12 ratios were higher in CD patients, and TGFβ/TNFα ratio was higher in HS group. IL-10, CD14+CD163+, TNF-α, and IL-10/IL-12 ratios with the AUC over 0.7 were introduced as fair diagnostic markers. Our findings revealed that the M2 (CD14+CD163+) monocytes were more frequent among CD patients, and the cytokine balance was disturbed. CONCLUSION: According to the significant functional diversities of monocyte subtypes between CD patients and HS group, these immunologic markers could be introduced as specific diagnostic biomarkers for CD.
Authors: N Matsushita; M Kashiwagi; R Wait; R Nagayoshi; M Nakamura; T Matsuda; P Hogger; P M Guyre; H Nagase; T Matsuyama Journal: Clin Exp Immunol Date: 2002-10 Impact factor: 4.330
Authors: Giacomo Caio; Umberto Volta; Anna Sapone; Daniel A Leffler; Roberto De Giorgio; Carlo Catassi; Alessio Fasano Journal: BMC Med Date: 2019-07-23 Impact factor: 8.775