Literature DB >> 344224

Acid precipitation of Clostridium botulinum type C and D toxins from whole culture by addition of ribonucleic acid as a precipitation aid.

M Iwasaki, G Sakaguchi.   

Abstract

The ratios of ribonucleic acid to protein contents of Clostridium botulinum type C, D, and E cultures were lower than those of type A, B, and F cultures. Addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type C and D toxins, but not that of type E toxin.

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Year:  1978        PMID： 344224      PMCID： PMC414144          DOI： 10.1128/iai.19.2.749-751.1978

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  11 in total

1.  Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid.

Authors:  M A CARDELLA; J T DUFF; B H WINGFIELD; C GOTTFRIED
Journal:  J Bacteriol       Date:  1960-03       Impact factor: 3.490

2.  Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.

Authors:  M GORDON; M A FIOCK; A YARINSKY; J T DUFF
Journal:  J Bacteriol       Date:  1957-10       Impact factor: 3.490

3.  Studies on immunity to toxins of Clostridium botulinum. IV. Production and purification of type C toxin for conversion to toxoid.

Authors:  M A CARDELLA; J T DUFF; C GOTTFRIED; J S BEGEL
Journal:  J Bacteriol       Date:  1958-03       Impact factor: 3.490

4.  Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties.

Authors:  S Miyazaki; M Iwasaki; G Sakaguchi
Journal:  Infect Immun       Date:  1977-08       Impact factor: 3.441

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  Purification and some properties of Clostridium botulinum type-E toxin.

Authors:  M Kitamura; S Sakaguchi; G Sakaguchi
Journal:  Biochim Biophys Acta       Date:  1968-10-21

7.  Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum.

Authors:  D A Boroff; U Fleck
Journal:  J Bacteriol       Date:  1966-11       Impact factor: 3.490

8.  Purification and some properties of progenitor toxins of Clostridium botulinum type B.

Authors:  S Kozaki; S Sakaguchi; G Sakaguchi
Journal:  Infect Immun       Date:  1974-10       Impact factor: 3.441

9.  Molecular construction of Clostridium botulinum type A toxins.

Authors:  S Sugii; G Sakaguchi
Journal:  Infect Immun       Date:  1975-12       Impact factor: 3.441

10.  Purification of Clostridium botuliunum type F progenitor toxin.

Authors:  I Oishi; G Sakaguchi
Journal:  Appl Microbiol       Date:  1974-12
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  6 in total

Review 1.  Clostridium botulinum neurotoxin.

Authors:  H Sugiyama
Journal:  Microbiol Rev       Date:  1980-09

2.  Evidence that botulinum C2 toxin has two dissimilar components.

Authors:  M Iwasaki; I Ohishi; G Sakaguchi
Journal:  Infect Immun       Date:  1980-08       Impact factor: 3.441

3.  Oral toxicities of Clostridium botulinum type C and D toxins of different molecular sizes.

Authors:  I Ohishi; G Sakaguchi
Journal:  Infect Immun       Date:  1980-05       Impact factor: 3.441

4.  Stabilities of Clostridium botulinum type B and C toxins in ruminal contents of cattle.

Authors:  S Kozaki; S Notermans
Journal:  Appl Environ Microbiol       Date:  1980-07       Impact factor: 4.792

5.  Rapid method for purification of Clostridium botulinum type C neurotoxin by high performance liquid chromatography (HPLC).

Authors:  M Matsuda; K Ozutsumi; P Y Du; N Sugimoto
Journal:  Eur J Epidemiol       Date:  1986-12       Impact factor: 8.082

6.  Purification and some properties of Aeromonas hydrophila hemolysin.

Authors:  T Asao; Y Kinoshita; S Kozaki; T Uemura; G Sakaguchi
Journal:  Infect Immun       Date:  1984-10       Impact factor: 3.441
  6 in total

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