Primary Laparoscopic Surgery Does Not Affect the Prognosis of Early-Stage Ovarian Clear Cell Cancer.

PURPOSE: Minimally invasive surgery (MIS) is performed frequently in early-stage ovarian cancer patients, especially in ovarian clear cell carcinoma (OCCC). The aim of this study was to investigate whether primary laparoscopic surgery influences prognosis in patients with early-stage OCCC. PATIENTS AND METHODS: Patients with International Federation of Gynecology and Obstetrics (FIGO) stage I  OCCC were retrospectively reviewed in two hospitals between April 2010 and August 2020. Clinical data were abstracted, and patients were followed up until February 2021. Patients were divided into open surgery (laparotomy) and laparoscopy groups, and the Kaplan-Meier method was applied to compare progression-free survival (PFS) and overall survival (OS) between the groups. Statistical differences were determined by the Log rank test.
RESULTS: Eighty-nine patients were included in the study; 20 (22.5%) and 69 (77.5%) patients underwent laparoscopic and open surgery, respectively. The patients' characteristics were well-balanced except that patients in the laparoscopy group tended to have smaller tumors and lower frequency of omentectomy and lymphadenectomy compared with the open surgery group. The median follow-up duration was 42.6 and 36.5 months in the laparoscopy and open surgery groups, respectively. Nine (10.1%) patients developed recurrence, and 4 (4.5%) died of the disease; all in the open surgery group. The estimated 2-year PFS rates were 100.0% and 90.1%, and the estimated 5-year OS rates were 100.0% and 91.9% in the laparoscopy and open surgery groups, respectively. No significant survival differences were found between the groups.
CONCLUSION: Survival was not compromised when primary laparoscopic surgery was performed in early-stage OCCC patients. A well-designed randomized controlled trial is warranted.

Introduction

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy and ranks fifth in cancer deaths among women.1 Ovarian clear cell cancer (OCCC), a subtype accounting for approximately 5–25% of all EOCs, is always diagnosed at an early stage and is confined to the ovary as a pelvic mass. Previous studies have shown that OCCC is relatively less aggressive in the early stage2 but may develop chemo-resistance in the advanced stage, leading to a significantly poorer prognosis compared with high-grade serous ovarian cancer.3,4

With developments in minimally invasive techniques, especially the wide use of laparoscopy, more gynecologic oncologists are considering minimally invasive surgery (MIS) in select patients.5 MIS was first applied to assess tumor burden and to evaluate the resectability of advanced-stage ovarian cancer.6 Recent studies have investigated the feasibility of MIS for interval debulking surgery after neoadjuvant chemotherapy7,8 and for secondary cytoreductive surgery in appropriate recurrent ovarian cancer patients.9–12 Additionally, for patients with apparent early-stage ovarian cancer, MIS has been applied for full staging surgery.13–16 However, as the LACC trial suggested that MIS was associated with lower progression-free survival (PFS) and overall survival (OS) compared with laparotomy in early-stage cervical cancer,17 gynecological oncologists began to pay attention to patients’ survival, which may be affected by MIS, especially in ovarian cancers. Furthermore, as OCCC is associated with endometriosis and because most OCCC patients are diagnosed in the early stage, MIS appears to be applied more frequently in this subtype of ovarian cancer.

Herein, we conducted a retrospective study involving consecutive International Federation of Gynecology and Obstetrics (FIGO) stage I OCCC patients. Each patient’s clinical characteristics, treatment, and prognosis data were carefully collected and analyzed. Specifically, we divided the patients into two groups, namely a laparoscopy group and an open surgery group, according to whether primary laparoscopic surgery was performed. We aimed to investigate whether MIS impacts the survival of patients with FIGO stage I OCCC, compared with open surgery.

Patients and Methods

Study Subjects

This was a two-center, retrospective, cohort study conducted in Fudan University Zhongshan Hospital and Zhejiang Cancer Hospital between April 2010 and August 2020. Data for patients who were pathologically confirmed as having OCCC were reviewed and collected. In this study, only patients diagnosed as FIGO stage I OCCC were included. This study was approved by the medical ethics committees of Fudan University Zhongshan Hospital and Zhejiang Cancer Hospital. The need for written informed consent was waived owing to the retrospective anonymized data collection. The private information of all enrolled patients was carefully protected, and the study was conducted in accordance with the guidelines of the Declaration of Helsinki.

Study Protocol

Medical records were abstracted to obtain the patients’ age at diagnosis, preoperative serum cancer antigen 125 (CA125) value, preoperative CA199 value, FIGO stage, type of surgery (open or laparoscopic), tumor size, tumor location, ascites volume, postoperative adjuvant chemotherapy, chemotherapy cycles, PFS, and OS. Patients were followed up every 3 months for the first 2 years, then every 6 months for the next 3 years, and annually, thereafter. The last follow-up date was February 2021.

Definitions

In our study, patients were divided into two groups, namely a laparoscopy group and a laparotomy (open surgery) group. The laparoscopy group was defined as undergoing primary laparoscopic surgery, including full laparoscopic staging surgery and conversion to open surgery. The laparotomy group was defined as undergoing open surgery directly. PFS was defined as the time from the primary surgery to the date of recurrence, and OS was calculated as the time from the primary surgery to the date of death.

Statistical Analysis

The SPSS software package for windows (version 19.0; IBM Corp., Armonk, NY, USA) was used for statistical analysis. Quantitative data were expressed as medians. The Kaplan–Meier method was used to compare survival between the two groups, and the statistical differences were determined by the Log rank test. A p-value < 0.05 was considered statistically significant.

Results

Baseline and Patients’ Characteristics

Eighty-nine patients diagnosed as FIGO stage I OCCC were included in this study. The median age was 51.0 years (range, 32–75 years); 37 (41.6%) patients were diagnosed as FIGO stage IA and 56.2% as stage IC. The median preoperative CA125 and CA199 values were 43.5 U/mL and 20.3 U/mL, respectively. Most (60.7%) patients had a tumor size > 80 mm, and 18.0% had an ascites volume of > 200 mL. Almost all patients (97.8%) had a unilateral tumor except for two cases. After primary staging surgery, 82 (92.1%) patients received platinum-based chemotherapy, and 74 (83.1%) patients received four or more cycles of chemotherapy, while six (6.7%) patients did not undergo chemotherapy.

Overall, 20 (22.4%) patients underwent primary laparoscopy as the primary staging surgery, and 69 (77.6%) patients underwent open surgery directly. As shown in Table 1, clinical characteristics, namely age at diagnosis, FIGO stage, preoperative CA125 and CA199 values, tumor location, ascites volume, and chemotherapy cycles were well-balanced between the two groups. Patients with large tumor size (> 8 cm) tended to undergo open surgery (p=0.003).

Table 1

Clinical Characteristics

Characteristics	N=89	Laparoscopy (N=20)	Laparotomy (N=69)	P value
Median age (years)	51.0	49.0	53.0	0.124
FIGO stage
IA	37 (41.6%)	11 (55.0%)	26 (37.7%)
IB	2 (2.2%)	1 (5.0%)	1 (1.4%)
IC	50 (56.2%)	8 (40.0%)	42 (60.9%)	0.203
Median CA125 (U/mL)	43.5	25.0	63.0	0.393
Median CA199 (U/mL)	20.3	9.9	25.2	0.232
Tumor size (mm)
≤ 80	29 (32.6%)	10 (50.0%)	19 (27.5%)
> 80	54 (60.7%)	6 (30.0%)	48 (69.6%)
NA	6 (6.7%)	4 (20.0%)	2 (3.0%)	0.003
Ascites
None	72 (80.9%)	19 (95.0%)	53 (76.8%)
Yes	16 (18.0%)	1 (5.0%)	15 (21.7%)
NA	1 (1.1%)	0	1 (1.4%)	0.106
Laterality
Unilateral	87 (97.8%)	19 (95.0%)	68 (98.6%)
Bilateral	2 (2.2%)	1 (5.0%)	1 (1.4%)	0.401
Chemotherapy
Platinum-based	82 (92.1%)	19 (95.0%)	63 (91.3%)
Others	1 (1.1%)	0	1 (1.4%)
No chemo	6 (6.7%)	1 (5.0%)	5 (7.2%)	0.787
Chemo cycles
0	6 (6.7%)	1 (5.0%)	5 (7.2)
1–3	9 (10.1%)	4 (20.0%)	5 (7.2%)
≥ 4	74 (83.1%)	15 (75.0%)	59 (85.5%)	0.245

Abbreviations: FIGO, International Federation of Gynecology and Obstetrics; NA, not acquired.

Surgical Procedures

Surgical procedures for the entire cohort are summarized in Table 2. Overall, 80 (89.9%) patients underwent hysterectomy, and 9 (10.1%) patients underwent fertility-preserving surgery. There was no statistically significant difference in the rates of hysterectomy, salpingo-oophorectomy, peritoneal biopsy, and appendectomy. Omentectomy (75.0% versus 100.0%; p < 0.001) and lymphadenectomy (50.0% versus 81.2%; p = 0.009) were less frequent in the laparoscopy group versus the open surgery group, respectively. Of the 66 patients receiving lymphadenectomy, 19 (28.8%) and 3 (4.5%) patients underwent only pelvic or para-aortic lymphadenectomy, respectively, and 44 (66.7%) patients underwent systematic lymphadenectomy. Of the 20 patients who underwent laparoscopic surgery, 10 (50.0%) were converted to laparotomy immediately, and 2 (10.0%) underwent delayed open surgery staging.

Table 2

Surgical Procedures

Variable	N=89	Laparoscopy (N=20)	Laparotomy (n=69)	P value
Surgical procedures
Hysterectomy	80 (89.9%)	17 (85.0%)	63 (91.3%)	0.414
Mono/Bilateral SO	89 (100.0%)	20 (100.0%)	69 (100.0%)	1.000
Omentectomy	84 (94.4%)	15 (75.0%)	69 (100.0%)	0.000
Peritoneal biopsy	63 (70.8%)	11 (55.0%)	52 (75.4%)	0.097
Appendectomy	8 (9.0%)	2 (10.0%)	6 (8.7%)	1.000
Lymphadenectomy	66 (74.2%)	10 (50.0%)	56 (81.2%)	0.009
Pelvic only	19 (28.8%)	4 (40.0%)	15 (26.8%)
Aortic only	3 (4.5%)	0	3 (5.4%)
Pelvic & Aortic	44 (66.7%)	6 (60.0%)	38 (67.9%)
Laparotomic conversion
No		8 (40.0%)
Yes (Immediate staging)		10 (50.0%)
Yes (Delayed staging)		2 (10.0%)

Abbreviation: SO, salpingo-oophorectomy.

Prognosis Between the Laparoscopy and Laparotomy Groups

As of February 2021, the median follow-up duration for the entire cohort was 40.7 months (range, 6.6–108.9 months), and 42.6 months and 36.5 months in the laparoscopy and open surgery groups, respectively. Overall, 9 (10.1%) patients developed recurrence, and 4 (4.5%) died of the disease; all were in the open surgery group. As shown in Figure 1A, the estimated 2-year PFS rates were 100.0% and 90.1% in the laparoscopy and open surgery groups, respectively (p = 0.081). There was also no significant difference in OS, with an estimated 5-year OS rate of 100.0% and 91.9% in the laparoscopy and open surgery groups, respectively (p = 0.230) (Figure 1B).

Figure 1

Kaplan–Meier plots for progression-free survival (A) and overall survival (B) between the laparoscopy and laparotomy groups.

Characteristics of the Recurrent Patients

The clinical characteristics of the recurrent patients are listed in Table 3. In summary, 7/9 recurrent patients were diagnosed as FIGO stage IC, and 2 were FIGO stage IA. Eight patients received lymph node resection, and one patient did not. All patients underwent six or more cycles of chemotherapy, and the time to recurrence ranged from 10.7 to 45.9 months. Recurrent lesions were found in the pelvic and abdominal peritoneum in three patients, local pelvic recurrence developed in two patients, and retroperitoneal lymph node recurrence developed in two patients. One patient was diagnosed with recurrence according to the presence of ascites. These patients’ treatments and status are listed in Table 3.

Table 3

Characteristics of Recurrent Patients

No	Age	Stage	LNR	Chemo	Time to Recurrence (Mos)	Recurrent Sites	Treatment After Relapse	Status
1	57	IC	Yes	DC*6	28.0	Peritoneum	Surgery+Chemo	AWD
2	72	IC	No	TC*7	45.9	Pelvic	Surgery+Chemo	AWD
3	55	IC	Yes	TC*6	13.6	Liver parenchyma	Chemo	Dead
4	49	IC	Yes	TC*6	30.2	Lymph nodes	Surgery+Chemo	AWD
5	54	IC	Yes	TC*6	19.2	Ascites	Chemo	AWD
6	42	IA	Yes	TC*6	15.0	Peritoneum	Surgery+Chemo	Dead
7	48	IC	Yes	TC*6	12.6	Peritoneum	Chemo	Dead
8	51	IC	Yes	AC*7	12.0	Lymph nodes	Chemo	Dead
9	46	IA	Yes	TC*6	10.7	Pelvic	Surgery+Chemo	NED

Abbreviations: LNR, lymph node resection; DC, Docetaxel + Carboplatin; TC, Paclitaxel + Carboplatin; AC, Doxorubicin + Carboplatin; AWD, alive with disease; NED, no evidence of disease.

Discussion

Although MIS is widely applied in gynecological surgery, including to diagnose endometriosis, the impact on endometriosis-associated ovarian cancer, including OCCC, which is frequently misdiagnosed as early-stage ovarian endometrioid cyst, remains uncertain. The potential risks of MIS in ovarian cancer may include the following: First, laparoscopy may fail to evaluate tumor disease because of severe dense adhesions, and occult tumor lesions, such as on the posterior surface of the diaphragm that are expected to be identified by palpation during open surgery, may be neglected during MIS.18 Second, MIS may cause intraoperative cancer cell spillage, leading to peritoneal dissemination or port-site metastasis.19–21 Third, we still do not know if carbon dioxide (CO2) pneumoperitoneum changes the tumor environment or the biological behavior of tumor cells; thus, promoting tumor spread or metastasis.

We found no survival differences, when we reviewed previous studies comparing the survival of patients with early-stage ovarian cancer between laparoscopic and open surgery groups; however, the study designs or the included patients differed in the studies.22–28 As shown in Table 4, most previous studies included patients with early EOC, and only one study focused on stage IC OCCC patients.25 In Chang et al’s study, 88 patients with stage IC OCCC were included, and 76 (86.4%) and 12 (13.6%) underwent direct exploratory laparotomy staging and laparoscopy, respectively.25 All 12 patients who underwent laparoscopic staging were converted to open surgery after pathological confirmation, and no survival differences were identified between the groups. The authors’ concluded that a laparoscopic diagnosis did not worsen patients’ survival if direct open conversion was performed.

Table 4

References Comparing Survival Between Open Surgery and MIS for Ovarian Cancer Patients with Early Stage

Ref.22–28	Patients	Group	Patients Number	Follow-Up (Months)	PFS Rate	OS Rate	Survival Difference
Ghezzi F. 2007	EOC	Open surgery	19	60 (32–108)	92.9%	100%
		Laparoscopy	15	16 (4–33)	100%	100%	NA
Park JY. 2008	EOC	Open surgery	33	23 (1–44)	100%	100%
		Laparoscopy	19	17 (2–40)	100%	100%	NA
Minig L, 2016	EOC	Open surgery	58	34.3 (28.4–47.8)	88% (51/58)	NA
		Laparoscopy	50	25.9 (11.2–38.5)	88% (44/50)	NA	N.S
Gallotta V, 2016	EOC	Open surgery	120	38 (24–48)	4-year: 81%	4-year: 91%
		Laparoscopy	60	38 (24–48)	4-year: 89%	4-year: 92%	N.S
Ditto A. 2017	EOC	Open surgery	50	52.6 (±81.8)	NA	NA
		Laparoscopy	50	49.5 (± 64)	NA	NA	N.S
Chang HT. 2020	OCCC	Open surgery	76	NA	73.7% (56/76)	86.8% (66/76)
		Laparoscopy	12	NA	83.3% (10/12)	91.7% (11/12)	N.S
Merlier M. 2020	EOC	Open surgery	107	42 (24.0–66.0)	71% (76/107)	84.1% (90/107)
		Laparoscopy	37	24 (11.0–50.0)	94.6% (35/37)	97.3% (36/37)	N.S

Abbreviations: MIS, minimally invasive surgery; EOC, epithelial ovarian cancer; OCCC, ovarian clear cell cancer; PFS, progression-free survival; OS, overall survival; NA, not acquired; N.S, none significance.

Differing from Chang et al’s study, we included OCCC FIGO stage IA–IC patients and patients who received full laparoscopic staging as well as those who were converted to open surgery. Twenty of 89 patients underwent laparoscopic staging, and 12 (60.0%) were converted to laparoscopic surgery directly or underwent delayed open surgery staging. Our results revealed no tumor recurrence in the laparoscopy group after a median follow-up of 42.6 months. These data may indicate that laparoscopic surgery has no impact on survival in stage I OCCC patients, regardless of whether the surgery was converted to open surgery.

Recently, a large study with a median follow-up of 61 months (range, 13–118 months) investigated the role of MIS for early-stage ovarian cancer patients.29 The authors concluded that grade 3 cancer was the most powerful prognostic factor for recurrence, whereas stage > IC was correlated with shorter PFS, but without reaching statistical significance. Tumor grade, final FIGO stage, and the time of surgical staging (immediate versus delayed) maintained an independent favorable prognostic role for PFS by multivariate analysis. In our study, we did not perform univariate or multivariate analysis for tumor recurrence because of the low recurrence number, and because the follow-up period was too short.

Importantly, we noticed that although no statistical significance was found in most previous studies, a shorter PFS or OS rate was observed in patients receiving direct open surgery staging in many recent studies.25,26 These data may be explained by different baseline characteristics, including preoperative imaging to determine large tumor size, which may lead to a choice of direct open surgery. However, no survival difference was identified between patients with tumor size ≤ 80 mm versus > 80 mm in our study (data not shown). A well-designed, randomized controlled trial should be conducted to resolve this question.

Conclusion

This was a retrospective study comparing survival between early-stage OCCC patients who underwent laparoscopy versus open surgery. Our study concluded that survival was not compromised when primary laparoscopic surgery was performed in FIGO stage I OCCC patients.