Detection of Long Noncoding RNA Expression by Real-Time PCR.

Recent advances in sequencing technology and bioinformatic analysis promote the identification of long noncoding RNA (lncRNA), a novel form of untranslated RNA transcript. Long coding RNA has been extensively investigated for its fundamental biological functions in a broad spectrum of pathogenetic and therapeutic studies. The level of lncRNA expression varies among different tissue types and contexts. Therefore, a quantitative method to accurately measure lncRNA expression is highly desired. Real-time PCR analysis enables us to conveniently determine the identity and abundance of lncRNA in a time-efficient manner. Here, we introduce how to use real-time PCR to analyze and quantify a defined lncRNA.