| Literature DB >> 34410647 |
Abstract
Many digestive tract microbes live adhered to tract epithelium. Work in recent years has brought the realization that these microbes and the host epithelial cells certainly must interact and that this interaction has an effect on both. One way to understand the interaction is to measure which genes are expressed in the epithelial cells and what bacteria are present. Even more informative would be to also determine what genes the bacteria express. Presented is a method to noninvasively isolate oral mucosal epithelium so to provide purified miRNA that can be used to profile miRNA expression specifically in the epithelium. miRNA is a major regulator of cell functions. Simultaneously, DNA and RNA from bacteria at the same site can be isolated to allow characterization of bacteria that coat the epithelial cells and extracellular matrix. This provides insight on the interaction between host and bacteria.Entities:
Keywords: 16s rRNA gene; Brush biopsy; Microbes; Oral mucosa; RNA stabilizers; miRNA
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Year: 2021 PMID： 34410647 DOI： 10.1007/978-1-0716-1518-8_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745