BACKGROUND: RNA expression analysis of oral keratinocytes can be used to detect early stages of disease such as oral cancer or to monitor on-going treatment responses of the same or other oral diseases. A limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression. METHODS: As a starting point in the analysis of tumor markers in oral squamous cell carcinoma (OSCC), we examined RNA in brush cytology samples from hamsters treated with dibenzo[a,l]pyrene to induce oral carcinoma. Three separate samples from each animal were assessed for expression of candidate marker genes and control genes measured with real-time RT-PCR. RESULTS: Brush oral cytology samples from normal mucosa were shown to consist almost exclusively of epithelial cells. Remarkably, ss-2 microglobulin and cytochrome p450, 1B1 (CYP1B1) RNA showed potential utility as markers of OSCC in samples obtained in this rapid and non-surgical manner. CONCLUSION: Brush oral cytology may prove useful as a source of RNA for gene expression analysis during the progression of diseases of the oral epithelium such as OSCC.
BACKGROUND: RNA expression analysis of oral keratinocytes can be used to detect early stages of disease such as oral cancer or to monitor on-going treatment responses of the same or other oral diseases. A limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression. METHODS: As a starting point in the analysis of tumor markers in oral squamous cell carcinoma (OSCC), we examined RNA in brush cytology samples from hamsters treated with dibenzo[a,l]pyrene to induce oral carcinoma. Three separate samples from each animal were assessed for expression of candidate marker genes and control genes measured with real-time RT-PCR. RESULTS: Brush oral cytology samples from normal mucosa were shown to consist almost exclusively of epithelial cells. Remarkably, ss-2 microglobulin and cytochrome p450, 1B1 (CYP1B1) RNA showed potential utility as markers of OSCC in samples obtained in this rapid and non-surgical manner. CONCLUSION: Brush oral cytology may prove useful as a source of RNA for gene expression analysis during the progression of diseases of the oral epithelium such as OSCC.
Authors: Shannon E Weigum; Pierre N Floriano; Spencer W Redding; Chih-Ko Yeh; Stephen D Westbrook; H Stan McGuff; Alan Lin; Frank R Miller; Fred Villarreal; Stephanie D Rowan; Nadarajah Vigneswaran; Michelle D Williams; John T McDevitt Journal: Cancer Prev Res (Phila) Date: 2010-03-23
Authors: Guy R Adami; Thomas N O'Callaghan; Antonia Kolokythas; Robert J Cabay; Yalu Zhou; Joel L Schwartz Journal: J Oral Pathol Med Date: 2016-12-18 Impact factor: 4.253
Authors: Guy R Adami; Alexander C F Yeung; Grant Stucki; Antonia Kolokythas; Herve Y Sroussi; Robert J Cabay; Igor Kuzin; Joel L Schwartz Journal: Arch Oral Biol Date: 2014-01-04 Impact factor: 2.633
Authors: Antonia Kolokythas; Mitchell J Bosman; Kristen B Pytynia; Suchismita Panda; Herve Y Sroussi; Yang Dai; Joel L Schwartz; Guy R Adami Journal: J Oral Pathol Med Date: 2013-04-17 Impact factor: 4.253