Next-Generation Sequencing in Cancer.

OBJECTIVE: In this article, we provide a gestalt idea about NGS technologies and their applications in cancer research and molecular diagnosis.
BACKGROUND: Next-generation sequencing (NGS) advancements like DNA sequencing and RNA sequencing allow uncovering of genomic, transcriptomic, and epigenomic scenes of individual malignant growths. An assortment of genomic abnormalities can be screened at the same time, for example common and uncommon variations, auxiliary variations like insertions and deletions, copy-number variation, and fusion transcripts.
CONCLUSION: NGS innovations together with bioinformatics investigation, which extend our insight, are progressively used to analyze multiple genes in a cost-effective way and have been applied in examining clinical cancer samples and offering NGS-based molecular diagnosis. APPLICATION: NGS is progressively significant as a device for the diagnosis of cancers. © The Association of Oral and Maxillofacial Surgeons of India 2020.