| Literature DB >> 34386960 |
Katherine D Krause1, Hsin-Yun Tsai1, Kelly Rees1, Hyungki Kim1, W Russ Algar2.
Abstract
Quantum dots (QDs) offer bright and robust photoluminescence among several other advantages in comparison to fluorescent dyes. In order to leverage the advantageous properties of QDs for applications in bioanalysis and imaging, simple and reliable methods for bioconjugation are required. One such method for conjugating peptides to QDs is the use of polyhistidine tags, which spontaneously bind to the surface of QDs. We describe protocols for assembling polyhistidine-tagged peptides to QDs and for characterizing the resultant QD-peptide conjugates. The latter include both electrophoretic and FRET-based protocols for confirming successful peptide assembly, estimating the maximum peptide loading capacity, and measuring the assembly kinetics. Sensors for protease activity and intracellular delivery are briefly noted as prospective applications of QD-peptide conjugates.Entities:
Keywords: Agarose gel electrophoresis; Förster resonance energy transfer (FRET); Peptides; Polyacrylamide gel electrophoresis; Polyhistidine; Quantum dots
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Year: 2021 PMID： 34386960 DOI： 10.1007/978-1-0716-1617-8_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745