Literature DB >> 3434800

Hydrochloric acid hydrolysis of proteins and determination of tryptophan by reversed-phase high-performance liquid chromatography.

L T Ng1, A Pascaud, M Pascaud.   

Abstract

A simple method was developed for determination of tryptophan in proteins. Hydrolysis is achieved under reducing conditions, in 6 N hydrochloric acid containing 0.4% beta-mercaptoethanol, at 110 degrees C for 24 h. The phenylthiocarbamyl derivatives of the amino acids are separated by reversed-phase high-performance liquid chromatography, without any by-product interference. The recovery of tryptophan is complete. However, the method does not allow the determination of tryptophan in carbohydrate-rich biological samples.

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Year:  1987        PMID： 3434800     DOI： 10.1016/0003-2697(87)90132-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  The metabolic network of Lactococcus lactis: distribution of (14)C-labeled substrates between catabolic and anabolic pathways.

Authors:  L Novák; P Loubiere
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry.

Authors:  Shrikaar Kambhampati; Jia Li; Bradley S Evans; Doug K Allen
Journal:  Plant Methods       Date:  2019-05-11       Impact factor: 4.993

3.  High-Throughput Analysis of Amino Acids for Protein Quantification in Plant and Animal-Derived Samples Using High Resolution Mass Spectrometry.

Authors:  Priyanka Reddy; Aaron Elkins; Joe Panozzo; Simone J Rochfort
Journal:  Molecules       Date:  2021-12-14       Impact factor: 4.411
  3 in total

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