Thyroid cancer is the most common thyroid malignancy, accounting for 2.6% of all malignant tumors (1). The majority of thyroid cancer types originate from follicular epithelial cells, and are mainly divided into papillary carcinoma and follicular adenocarcinoma, according to their pathological subtypes (2). Available treatment methods exist, such as surgical treatment (3), thyroid hormone suppression therapy (4) and radioiodine ablation (5) that may effectively inhibit tumors. However, secondary recurrence is common (6). Thus, it is important to identify effective biomarkers and targets to improve the survival rate of patients with thyroid cancer.Circular RNAs (circRNAs) are non-coding RNAs that play important roles in the development of several diseases, such as cardiovascular disease, nervous system disease and cancer, as well as regulate specific cellular functions, including development, proliferation and invasion (7,8). Lee et al (7) demonstrated the roles of circRNAs in the development of human diseases. Furthermore, Ma et al (8) revealed the potential regulatory role of circRNA-000284 in cervical cancer cell proliferation and invasion by targeting microRNA (miRNA/miR)-506. Previous studies have reported that circRNAs contain several miRNA binding sites, which are involved in the regulation of gene expressions by sponging miRNAs (9,10). circRNA_0000285 has been identified as a novel circRNA, which promotes the metastasis and invasion of several types of cancer by targeting specific miRNAs (11). However, the roles and underlying molecular mechanism of circRNA_0000285 in thyroid cancer remain unclear.miRNAs are a class of endogenous small RNAs with vital regulatory roles in cells (12). It has been reported that miRNAs are promising markers of several biological processes, including cell proliferation, migration and invasion (13). For example, miR-211 triggers an autophagy-dependent apoptotic response in cervical cancer cells by regulating B-cell lymphoma 2 (Bcl-2) (14). Furthermore, miR-654-3p is a key regulator of cell proliferation and migration that promotes the induction of gene reprogramming (15). miR-654-3p is a tumor inhibitory factor that is downregulated in hepatocellular carcinoma (16). Pu et al (17) highlighted that miR-654-3p suppresses non-small cell lung cancer tumorigenesis by inhibiting polo like kinase 4. However, whether miR-654-3p is associated with the development of thyroid cancer remains unknown. Notably, a previous study revealed the interaction sites between circRNA_0000285 and miR-654-3p, and circRNA_0000285 functions as a sponge of miR-654-3p in diabetic nephropathy (18). However, the association between circRNA_0000285 and miR-654-3p in thyroid cancer has not been studied till now.The present study aimed to investigate the roles and underlying molecular mechanism of circRNA_0000285, and determine the association between circRNA_0000285 and miR-654-3p in thyroid cancer to identify novel strategies for thyroid cancer treatment.
Materials and methods
Cell culture
The cell lines, TPC-1, FTC133 and Nthy-ori 3-1, were purchased from the American Type Culture Collection and maintained in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (HyClone; Cytiva) and 1% penicillin-streptomycin (Gibco; Thermo Fisher Scientific, Inc.), at 37°C with 5% CO2.
Dual-luciferase reporter assay
StarBase version 2.0 (http://starbase.sysu.edu.cn/index.php) was used to predict the binding sites between circRNA_0000285 and miR-654-3p. The dual-luciferase reporter assay was performed to confirm the binding sites between circRNA_0000285 and miR-654-3p. The wild-type 3′-untranslated region (UTR) of circRNA_0000285 (WT circ_0000285: 5′-GGUGAUGCUUUUCAGCAGACAUU-3′), which contains the miR-654-3p binding site or the mutated target site (MUT circ_0000285: 5′-GGUGAUGCUUUUCAGGUCUGUAU-3′) was generated by PCR (using a Transcriptor First Strand cDNA Synthesis kit (cat. no. 04896866001; Roche Molecular Diagnostics) (incubation for 5 min at 25°C followed by 60 min at 42°C) from total RNA preps extracted from TPC-1 cells (electrophoresis analysis, 1% agarose gel; visualisation method, ethidium bromide) and cloned into the pMIR vectors (Ambion; Thermo Fisher Scientific, Inc.) to construct the reporter vector circRNA_0000285 wild-type or the circRNA_0000285 mutant-type. 293T cells (American Type Culture Collection) were transfected with circRNA_0000285 wild-type or the mutant sequence, and with miR-654-3p mimic (forward, 5′-UGGUGGGCCGCAGAACAUGUGC-3′ and reverse, 5′-ACAUGUUCUGCGGCCCACGAAU-3′) or mimic control (forward, 5′-UUCUCCGAACGUGUCACGUUU-3′ and reverse, 5′-ACGUGACACGUUCGGAGAAUU-3′; both purchased from Shanghai GenePharma, Co., Ltd.) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 48 h, according to the manufacturer's instruction. Following cell transfection, 48 h later the Dual-Luciferase Reporter Assay system (Promega Corporation) was applied to measure luciferase activity and Renilla luciferase was used as a normalization control.
RNA pull-down assay
The pull-down assay was performed as previously described (19). Briefly, the biotinylated circRNA_0000285 probe and oligo probe (Shanghai GenePharma Co., Ltd.) were incubated with M-280 Streptavidin magnetic beads (cat. no. 60210; Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instruction for 2 h at room temperature to generate probe-coated beads. Subsequently, the biotinylated RNA coated beads were separated with a magnet for 3 min and washed with 1X B&W Buffer [2X B&W Buffer: 10 mM Tris-HCl (pH 7.5) 1 mM EDTA 2 M NaCl]. TPC-1 and FTC133 cells (1×107 cells) were harvested, lysed in 500 µl lysis buffer (Beyotime Institute of Biotechnology), sonicated and incubated with probe-coated beads overnight at 4°C. After washing with 1X B&W Buffer, the RNA complexes bound to the beads were eluted and extracted using the RNA isolation kit (Thermo Fisher Scientific, Inc.), and analyzed via reverse transcription-quantitative (RT-q)PCR analysis.The pull-down efficiency was verified in TPC-1 and FTC133 cells transfected with circRNA_0000285 (human circRNA_0000285 cDNA was synthesized and cloned into the plenti-ciR-GFP-T2A vector) or vector (plenti-ciR-GFP-T2A vector; IGE Biotech Co., Ltd.) using Lipofectamine RNAiMax® (Thermo Fisher Scientific, Inc.) according to the manufacturer's instruction.
RNA immunoprecipitation (RIP) assay
The RIP assay is a powerful tool used to assess the binding of RNA molecules to proteins in cells and to assess the dynamic process of post transcriptional regulatory networks (20). Thyroid cancer cells were transfected with miR-654-3p mimic and mimic control at 37°C for 48 h, and were lysed using RIP buffer (Beyotime Institute of Biotechnology). The magnetic beads were coated with anti-Ago2 (1:50; cat. no. ab186733; Abcam) or IgG (1:300; cat. no. ab109489; Abcam) antibodies and the cell lysate was incubated with the magnetic beads at 4°C for 1 h, according to the manufacturer's instructions.
RT-qPCR
RNA was extracted from thyroid cancer cells and normal thyroid cells using the RNA isolation kit (Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions. cDNA was synthesized using the PrimeScript RT kit (Takara Bio, Inc.) and amplified via qPCR on an ABI PRISM 7900 sequence detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.), with SYBR Premix Ex-Taq (Takara Bio, Inc.). The thermocycling conditions were as follows: Initial denaturation at 95°C for 5 min, followed by 38 cycles of denaturation at 95°C for 15 sec and annealing/elongation at 60°C for 30 sec. The following primer sequences (Sangon Biotech Co., Ltd.) were used for qPCR: U6 forward, 5′-CTCGCTTCGGCAGCACA-3′ and reverse, 5′-AACGCTTCACGAATTTGCGT-3′; GAPDH forward, 5′-TCAACGACCACTTTGTCAAGCTCA-3′ and reverse, 5′-GCTGGTGGTCCAGGGGTCTTACT-3′; miR-654-3p forward, 5′-GGGATGTCTGCTGACCA-3′ and reverse, 5′-CAGTGCGTGTCGTGGA-3′; and circRNA_0000285 forward, 5′-TACCTCTGCAGGCAGGAACT-3′ and reverse, 5′-TCACATGAATTTAGGTGGGACTT-3′. Relative expression levels were calculated using the 2−ΔΔCq method (21).
Cell transfection
Control-small interfering (si)RNA (1 µg, 5′-AAGACAUUGUGUGUCCGCCTT-3′), circRNA_0000285-siRNA (1 µg, 5′-CCCCAGCUAUUCAAGUGUAAA−3′), inhibitor control (50 nM, 5′-AAGUCAGGUGAUGGACAGCAUA-3′) and miR-654-3p inhibitor (50 nM, 5′-AAGGUGAUGGUCAGCAGACAUA-3′) (all purchased from Shanghai GenePharma Co., Ltd.) were transfected into TPC-1 and FTC133 cells using Lipofectamine 2000® (Thermo Fisher Scientific, Inc.) at 37°C for 48 h, according to the manufacturer's instructions. After 48 h, RT-qPCR analysis was performed to assess the efficiency of transfection.
MTT assay
Following transfection, TPC-1 and FTC133 cells were cultured for 48 h and seeded into 96-well plates. The plates were incubated for 24, 48 and 72 h at 37°C. Subsequently, cells were treated with 10 µl MTT (5 mg/ml) solution and incubated for an additional 4 h. Following treatment, the solution was removed and 100 µl DMSO was added to each well to dissolve the formazan product. Samples were vibrated using a Microplate Shaker (Thermo Fisher Scientific, Inc.) and optical density was measured at a wavelength of 570 nm, using a multifunctional plate reader (BioTek Instruments, Inc.), according to the manufacturer's instructions.
Flow cytometry
Following incubation of transfected TPC-1 and FTC133 cells for 48 h, the induction of apoptosis was measured using the Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit (BD Biosciences), according to the manufacturer's instructions. Flow cytometry was performed to detect cell apoptosis (early apoptotic cells + late apoptotic cells; upper right quadrant + lower right quadrant) and the results were analyzed using Kaluza Analysis (version 2.1.1.20653; Beckman Coulter, Inc.).
Western blotting
Following transfection, TPC-1 and FTC133 cells were incubated for 48 h and total proteins were extracted using RIPA buffer (Invitrogen; Thermo Fisher Scientific, Inc.). BCA assay (Thermo Fisher Scientific, Inc.) was used to measure the protein concentrations. Equal amounts of proteins (40 µg/lane) were separated via 12% SDS-PAGE, transferred onto PVDF membranes and blocked with 5% skimmed milk in PBS-0.1% Tween-20 at room temperature for 1.5 h. The membranes were incubated with primary antibodies against Bcl-2 (cat. no. 4223), Bax (cat. no. 5023) or GAPDH (cat. no. 5174) (all 1:1,000 and purchased from Cell Signaling Technology, Inc.) at 4°C overnight. Following the primary incubation, membranes were washed with PBST 3 times and incubated with anti-rabbit IgG horseradish peroxidase-linked antibody (cat. no. 7074; 1:2,000; Cell Signaling Technology, Inc.) at room temperature for 1.5 h. Protein bands were visualized using ECL detection system reagents (MilliporeSigma), according to the manufacturer's instructions. ImageJ v.2.0 software (National Institutes of Health) was used to quantify the band intensity.
Statistical analysis
Statistical analysis was performed using SPSS 20.0 software (IBM Corp.). All experiments were performed in triplicate and data are presented as the mean ± SD. Unpaired Student's t-test was used to compare differences between two groups, while one-way ANOVA followed by Tukey's post hoc test were used to compare differences between multiple groups. P<0.05 was considered to indicate a statistically significant difference.
Results
miR-654-3p directly interacts with circRNA_0000285
Previous studies have reported that circRNAs are involved in cancer development by regulating the expression of specific target genes (8,11). Initially, bioinformatics analysis was performed to predict the target gene of circRNA_0000285. miR-654-3p was revealed to be a latent target of circRNA_0000285 (Fig. 1A). Subsequently, the association between circRNA_0000285 and miR-654-3p was confirmed via the dual-luciferase reporter assay (Fig. 1B). Compared with the cells co-transfected with circRNA_0000285 wild-type and mimic control, the luciferase activity of cells co-transfected with circRNA_0000285 wild-type and miR-654-3p mimic were significantly reduced (P<0.01; Fig. 1B). While no significant changes were observed of the luciferase activity in cells co-transfected with circRNA_0000285 wild-type and mimic control and cells co-transfected with circRNA_0000285 wild-type and miR-654-3p mimic (Fig. 1B). The RNA pull-down assay was performed to confirm the binding between circRNA_0000285 and miR-654-3p, and the pull-down efficiency was verified in TPC-1 and FTC133 cells transfected with circRNA_0000285 or vector (Fig. 1C and D). Compared with Oligo probe, circRNA_0000285 probe significantly enhanced circRNA_0000285 level in TPC-1 and FTC133 cells (all P<0.01; Fig. 1C and D). miR-654-3p pulled down by biotinylated probes were purified and analyzed via RT-qPCR analysis. The results demonstrated that miR-654-3p was notably pulled down by circRNA_0000285 probe in both TPC-1 and FTC133 cells (Fig. 1E). The results of the RIP assay (Fig. 1F and G) verified that circRNA_0000285 can directly target miR-654-3p, evidenced by significant enhancement of circRNA_0000285 and miR-654-3p in the Anti-Ago2 group in TPC-1 and FTC133 cells compared with in the (all P<0.01; Fig. 1F and G). Taken together, these results suggest that circRNA_0000285 is a direct target of miR-654-3p.
Figure 1.
miR-654-3p is a direct target of circRNA_0000285. (A) Schematic diagram of the miR-654-3p binding site in the 3′-untranslated region of circRNA_0000285. (B) The association between miR-654-3p and circRNA_0000285 was verified via the dual-luciferase reporter assay. Relative circRNA_0000285 expression in (C) TPC-1 and (D) FTC133 lysates pulled down by circRNA_0000285 probe or oligo probe. (E) Relative miR-654-3p expression in TPC-1 and FTC133 lysates pulled down by circRNA_0000285 probe or oligo probe. (F and G) The RNA immunoprecipitation assay was performed using Ago2 and IgG antibodies to immunoprecipitate. The expression levels of circRNA_0000285 and miR-654-3p in TPC-1 and FTC133 cells were detected via reverse transcription-quantitative PCR analysis. **P<0.01 vs. mimic control; #P<0.05 vs. Oligo probe; ##P<0.01 vs. Oligo probe; &&P<0.01 vs. Anti-IgG. miR, microRNA; circRNA, circular RNA; WT, wild-type; MUT, mutant.
circRNA_0000285 expression is upregulated and miR-654-3p expression is downregulated in thyroid carcinoma cells
The expression levels of circRNA_0000285 and miR-654-3p in thyroid cancer cell lines were detected via RT-qPCR analysis. circRNA_0000285 expression was significantly upregulated (P<0.01; Fig. 2A) and miR-654-3p expression was significantly downregulated (P<0.01; Fig. 2B) in the thyroid cancer cell lines (TPC-1 and FTC133) compared with Nthy-ori 3-1 cells.
Figure 2.
Expression levels of circRNA_0000285 and miR-654-3p in thyroid carcinoma cells. (A) RT-qPCR analysis was performed to detect circRNA_0000285 expression in thyroid cancer cells. (B) RT-qPCR analysis was performed to detect miR-654-3p expression in thyroid cancer cells. ##P<0.01 vs. Nthy-ori 3-1 cells. circRNA, circular RNA; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR.
circRNA_0000285 negatively regulates miR-654-3p expression in thyroid cancer cells
To verify the role of circRNA_0000285 in thyroid cancer, control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into TPC-1 and FTC133 cells for 48 h. RT-qPCR analysis demonstrated that circRNA_0000285 expression significantly decreased in TPC-1 and FTC133 cells following transfection with circRNA_0000285-siRNA compared with the control-siRNA group (P<0.01; Fig. 3A and B). In addition, miR-654-3p expression significantly decreased in TPC-1 and FTC133 cells transfected with miR-654-3p inhibitor compared with the inhibitor control group (P<0.01; Fig. 3C and D). Notably, miR-654-3p expression significantly increased following transfection with circRNA_0000285-siRNA, the effect of which was reversed following addition of miR-654-3p inhibitor (P<0.01; Fig. 3E and F). Collectively, these results suggest that miR-654-3p interferes with circRNA_0000285 expression in thyroid cancer cells.
Figure 3.
miR-654-3p inhibitor reverses the effects of circRNA_0000285 on miR-654-3p expression. Control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into TPC-1 and FTC133 cells for 48 h. circRNA_0000285 expression was detected via RT-qPCR analysis in (A) TPC-1 and (B) FTC133 cells following transfection with circRNA_0000285-siRNA or control-siRNA. miR-654-3p expression was detected via RT-qPCR analysis in (C) TPC-1 and (D) FTC133 cells following transfection with miR-654-3p inhibitor or inhibitor control. RT-qPCR analysis was performed to detect miR-654-3p expression in (E) TPC-1 and (F) FTC133 cells transfected with circRNA 0000285-siRNA + inhibitor control or circRNA 0000285-siRNA + miR-654-3p inhibitor. **P<0.01 vs. control-siRNA; ##P<0.01 vs. inhibitor control; &&P<0.01 vs. si-circ + inhibitor control. miR, microRNA; circRNA, circular RNA; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering.
circRNA_0000285-siRNA inhibits cell proliferation and induces apoptosis by increasing miR-654-3p expression in TPC-1 cells
To further assess the underlying molecular mechanism between miR-654-3p and circRNA_0000285, control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into TPC-1 cells for 48 h to determine the effects of circRNA 0000285-siRNA on cell proliferation and apoptosis. The results from the MTT assay and flow cytometric analysis demonstrated that circRNA_0000285-siRNA significantly inhibited TPC-1 cell proliferation and promoted apoptosis (all P<0.01; Fig. 4A-C). The expression levels of the apoptosis-associated proteins, Bax and Bcl-2, were detected via western blot analysis. The results demonstrated that transfection with circRNA_0000285-siRNA significantly increased Bax expression and decreased Bcl-2 expression (P<0.01; Fig. 4D-F), and the ratio of Bax/Bcl-2 significantly increased compared with the control-siRNA group (P<0.01; Fig. 4G). Notably, these effects were reversed following transfection with circRNA_0000285-siRNA + miR-654-3p inhibitor (Fig. 4).
Figure 4.
miR-654-3p inhibitor reverses the effects of circRNA_0000285 on TPC-1 cell proliferation and apoptosis. Control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into TPC-1 cells for 48 h. (A) The viability of TPC-1 cells was assessed via the MTT assay. (B) Detection of apoptotic TPC-1 cells was assessed via flow cytometric analysis. (C) Quantitative analysis of apoptotic cells. (D) Western blot analysis was performed to detect the protein expression levels of Bax and Bcl-2 in the different groups. (E) Bax/GAPDH ratio and (F) Bcl-2/GAPDH ratio. (G) Assessment of the Bax/Bcl-2 ratio. **P<0.01 vs. control-siRNA; ##P<0.01 vs. si-circ + inhibitor control. miR, microRNA; circRNA, circular RNA; si, small interfering; Bcl-2, B-cell lymphoma 2; OD, optical density; PI, propidium iodide.
circRNA_0000285-siRNA inhibits cell proliferation and induces apoptosis by increasing miR-654-3p expression in FTC133 cells
The effects of circRNA_0000285-siRNA were assessed on FTC133 cell proliferation and apoptosis. Control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into FTC133 cells for 48 h. Transfection with circRNA_0000285-siRNA significantly decreased TPC-1 cell proliferation and promoted induction of TPC-1 cell apoptosis (P<0.01; Fig. 5A-C). Western blot analysis demonstrated that circRNA_0000285-siRNA significantly enhanced Bax expression (P<0.01; Fig. 5D and E) and decreased Bcl-2 expression (all P<0.01; Fig. 5D and F), and significantly increased the ratio of Bax/Bcl-2 compared with the control-siRNA group (P<0.01; Fig. 5G). Notably, these effects were reversed following transfection with circRNA_0000285-siRNA + miR-654-3p inhibitor. Taken together, these results suggest that circRNA 0000285-siRNA suppresses cell proliferation and induces apoptosis in thyroid cancer cells by targeting miR-654-3p.
Figure 5.
miR-654-3p inhibitor reverses the effects of circRNA_0000285 on FTC133 cell viability and apoptosis. Control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into FTC133 cells for 48 h. (A) The viability of FTC133 cells was assessed via the MTT assay. (B) Detection of apoptotic FTC133 cells was assessed via flow cytometric analysis. (C) Quantitative analysis of apoptotic cells. (D) Western blot analysis was performed to detect the protein expression levels of Bax and Bcl-2 levels in the different groups. (E) Bax/GAPDH ratio and (F) Bcl-2/GAPDH ratio. (G) Assessment of the Bax/Bcl-2 ratio. **P<0.01 vs. control-siRNA; ##P<0.01 vs. si-circ + inhibitor control. miR, microRNA; circRNA, circular RNA; si, small interfering; Bcl-2, B-cell lymphoma 2; OD, optical density; PI, propidium iodide.
Discussion
Thyroid cancer is an endocrine tumor with high morbidity and incidence rates (22) and 255,490 incident cases and 41,240 deaths were reported in 2017 worldwide (23). Over the past decade, increasing numbers of thyroid cancer cases have been reported (24). According to previous report, it is estimated that >64,300 newly diagnosed cases of thyroid cancer will emerge in the United States in 2016 (25). Thus, effective therapeutic methods are required to reduce the burden of patients with thyroid cancer.circRNAs are a type of non-coding RNAs that play important roles in oncogenesis, including thyroid cancer development (26,27). Increasing evidence suggest that circRNAs are involved in cellular processes, such as cell proliferation, apoptosis, differentiation and invasion, and that they may be used as novel biomarkers or therapeutic targets for disease treatment. For example, Zhang et al (28) confirmed that circ_0067934 depletion accelerates cell apoptosis and represses cell proliferation, migration and invasion in thyroid cancer by sponging miR-1304 and regulating C-X-CR motif chemokine receptor 1 expression. hsa_circ_0000285 is a novel circRNA that is dysregulated in various cancers and disease types, including osteosarcoma (29), diabetic nephropathy (30) and laryngocarcinoma (31). However, the specific role and molecular mechanism of circ_0000285 in thyroid cancer have not yet been fully investigated. Thus, the present study investigated the underlying molecular mechanism of circRNA_0000285 in thyroid cancer to identify novel treatment methods for this disease.A previous study revealed the interaction sites between circRNA_0000285 and miR-654-3p (18). However, the association between circRNA_0000285 and miR-654-3p in thyroid cancer remain unknow. To the best of our knowledge, the present study was the first to investigate the association between circRNA_0000285 and miR-654-3p in thyroid cancer. The present study confirmed the binding between circRNA_0000285 and miR-654-3p via the dual-luciferase reporter and RIP assays. The results revealed that miR-654-3p directly interacted with circRNA_0000285. The present study also assessed the role of circRNA_0000285 in thyroid cancer cells. Its expression was detected, along with miR-654-3p expression, in the thyroid cancer cell lines, TPC-1 and FTC133, and Nthy-oir 3-1 cells. The results demonstrated that circRNA_0000285 expression was upregulated, whereas miR-654-3p expression was downregulated in thyroid cancer cells compared with Nthy-ori 3-1 cells. Taken together, these results suggest that circ_0000285 functions as an oncogene in thyroid tumors by regulating miR-654-3p expression.Previous studies have reported that abnormal regulation of miRNAs is associated with the development and aggressiveness of several tumors (32–34). Altered expression levels of circRNA_0000285 or miR-654-3p may affect the functions of thyroid cancer cells. Control-siRNA, circRNA_0000285-siRNA, inhibitor control and miR-654-3p inhibitor sequences were transfected into TPC-1 and FTC133 cells for 48 h. Transfection efficiency was assessed via RT-qPCR analysis. The results demonstrated that circRNA_0000285-siRNA suppressed circRNA_0000285 expression in TPC-1 and FTC133 cells. In addition, transfection with miR-654-3p inhibitor significantly inhibited miR-654-3p expression, whereas circRNA_0000285-siRNA promoted miR-654-3p expression in TPC-1 and FTC133 cells compared with the control-siRNA group. Notably, these results were reversed following transfection with miR-654-3p inhibitor. Collectively, these results suggest that circRNA_0000285 negatively regulates miR-654-3p expression in TPC-1 and FTC133 cells.Defective apoptosis is a main characteristic of tumor cells, whereby induction of apoptosis may prevent cancer development (35). It has been reported that long non-coding RNA urothelial cancer associated 1 promotes gastric cancer proliferation, migration and inhibits apoptosis by sponging antitumor miRNAs (36). In the present study, transfection of thyroid cancer cells with circRNA_0000285-siRNA decreased cell viability, which was accompanied by increased cell apoptosis in the circRNA_0000285-siRNA group. These effects were reversed following transfection of cells with miR-654-3p inhibitor.Bax is a pro-apoptotic protein that is a transcriptional target for p53 (37). Bcl-2 can inhibit the activity of Bax and induce cell apoptosis (38). To further investigate the molecular mechanism of circRNA_0000285 and miR-654-3p in thyroid cancer cells, the expression levels of Bax and Bcl-2 were detected in the different groups. The results demonstrated that circRNA_0000285-siRNA enhanced Bax expression, decreased Bcl-2 expression and increased the ratio of Bax/Bcl-2 compared with the control-siRNA group. Notably, these effects were reversed following transfection with circRNA_0000285-siRNA + miR-654-3p inhibitor. Taken together, these results suggest that circRNA_0000285-siRNA inhibits thyroid cancer cell proliferation and induces apoptosis by increasing miR-654-3p expression.The present study was an in vitro preliminary study of the role of circRNA_0000285 in the proliferation and apoptosis of thyroid cancer cells. To confirm the role of circRNA_0000285 in thyroid cancer, in-depth experiments are required. For example, determining the role of miR-654-3p alone in thyroid cancer cells is required to verify the results presented here. In addition, the specific molecular mechanism of circRNA_0000285/miR-654-3p affecting the viability of thyroid cancer cells requires investigation. Prospective studies are also required to investigate other target genes downstream of circRNA_0000285 or miR-654-3p in thyroid cancer. Furthermore, the role of circRNA_0000285 in thyroid cancer should be determined in vivo.In conclusion, the results of the present study demonstrated that circRNA_0000285-siRNA repressed thyroid cancer cell proliferation and induced apoptosis by regulating miR-654-3p expression. These findings may provide evidence for a novel biomarker and a potential therapeutic target for thyroid cancer.
Authors: Andrei Buruiană; Ștefan Ioan Florian; Alexandru Ioan Florian; Teodora-Larisa Timiș; Carmen Mihaela Mihu; Maria Miclăuș; Sergiu Oșan; Iona Hrapșa; Radu Constantin Cataniciu; Marius Farcaș; Sergiu Șușman Journal: Int J Mol Sci Date: 2020-03-12 Impact factor: 5.923
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