Literature DB >> 34341193

The X-ray structure of L-threonine dehydrogenase from the common hospital pathogen Clostridium difficile.

Eyram Adjogatse1, Josh Bennett1, Jingxu Guo1, Peter T Erskine1, Steve P Wood1, Brendan W Wren2, Jonathan B Cooper1.   

Abstract

In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+ to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH from Clostridium difficile has been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 Å resolution.

Entities:  

Keywords:  Clostridium difficile; molecular replacement; protein crystallography; refinement; threonine dehydrogenase

Mesh：

Substances：

Year:  2021        PMID： 34341193      PMCID： PMC8329716          DOI： 10.1107/S2053230X21007135

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.072


  38 in total

1.  Two WXXF-based motifs in NECAPs define the specificity of accessory protein binding to AP-1 and AP-2.

Authors:  Brigitte Ritter; Alexei Yu Denisov; Jacynthe Philie; Christophe Deprez; Elaine C Tung; Kalle Gehring; Peter S McPherson
Journal:  EMBO J       Date:  2004-09-09       Impact factor: 11.598

2.  Highly selective L-threonine 3-dehydrogenase from Cupriavidus necator and its use in determination of L-threonine.

Authors:  Techawaree Ueatrongchit; Yasuhisa Asano
Journal:  Anal Biochem       Date:  2010-11-10       Impact factor: 3.365

3.  Molecular replacement with MOLREP.

Authors:  Alexei Vagin; Alexei Teplyakov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-12-21

4.  Linking crystallographic model and data quality.

Authors:  P Andrew Karplus; Kay Diederichs
Journal:  Science       Date:  2012-05-25       Impact factor: 47.728

5.  Mice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene.

Authors:  Alasdair J Edgar
Journal:  BMC Genomics       Date:  2005-03-09       Impact factor: 3.969

6.  Crystallization and preliminary X-ray analysis of hyperthermophilic L-threonine dehydrogenase from the archaeon Pyrococcus horikoshii.

Authors:  Noriko Higashi; Takanori Matsuura; Atsushi Nakagawa; Kazuhiko Ishikawa
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-04-01

7.  REFMAC5 for the refinement of macromolecular crystal structures.

Authors:  Garib N Murshudov; Pavol Skubák; Andrey A Lebedev; Navraj S Pannu; Roberto A Steiner; Robert A Nicholls; Martyn D Winn; Fei Long; Alexei A Vagin
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

8.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

Review 9.  Clostridium difficile Infections: A Global Overview of Drug Sensitivity and Resistance Mechanisms.

Authors:  Saeed S Banawas
Journal:  Biomed Res Int       Date:  2018-02-21       Impact factor: 3.411

10.  The human L-threonine 3-dehydrogenase gene is an expressed pseudogene.

Authors:  Alasdair J Edgar
Journal:  BMC Genet       Date:  2002-10-02       Impact factor: 2.797
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