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Literature DB >> 3433293

Determination of phospholipase A2 activity by a colorimetric assay using a pH indicator.

Abstract

We have set up an assay of phospholipase A2 by a spectrophotometric method, based on the pH change due to the liberation of fatty acids. Among the pH indicators used, phenol red was found to be one of the most sensitive. The activities of different phospholipases A2 from venom and from porcine pancreas were measured by this assay. The results are comparable to those obtained by the pH stat method. This very simple test is rapid, sensitive and especially useful for assaying numerous samples. For quantitative results in absolute units it must be considered that the pH indicator may inhibit some phospholipases.

Entities: Chemical Gene

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Year: 1987 PMID： 3433293 DOI： 10.1016/0041-0101(87)90136-x

Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033

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Cited

25 in total

1. cDNA sequence analysis and mutagenesis studies on the A chain of beta-bungarotoxin from Taiwan banded krait.

Authors: L S Chang; P F Wu; C C Chang
Journal: J Protein Chem Date: 1996-11

2. Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts.

Authors: Kammoun Maher; Ben Ali Yassine; Bezzine Sofiane
Journal: Afr Health Sci Date: 2015-06 Impact factor: 0.927

3. Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis.

Authors: Richard J Ward; Lucimara Chioato; Arthur H C de Oliveira; Roberto Ruller; Juliana M Sá
Journal: Biochem J Date: 2002-02-15 Impact factor: 3.857

4. Asp-49 is not an absolute prerequisite for the enzymic activity of low-M(r) phospholipases A2: purification, characterization and computer modelling of an enzymically active Ser-49 phospholipase A2, ecarpholin S, from the venom of Echis carinatus sochureki (saw-scaled viper).

Authors: J Polgár; E M Magnenat; M C Peitsch; T N Wells; K J Clemetson
Journal: Biochem J Date: 1996-11-01 Impact factor: 3.857

Determination of phospholipase A2 activity by a colorimetric assay using a pH indicator.

1. cDNA sequence analysis and mutagenesis studies on the A chain of beta-bungarotoxin from Taiwan banded krait.

2. Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts.

3. Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis.

4. Asp-49 is not an absolute prerequisite for the enzymic activity of low-M(r) phospholipases A2: purification, characterization and computer modelling of an enzymically active Ser-49 phospholipase A2, ecarpholin S, from the venom of Echis carinatus sochureki (saw-scaled viper).

5. The essentiality of calcium ion in the enzymatic activity of Taiwan cobra phospholipase A2.

6. Comparison of the biological properties of several marine sponge-derived sesquiterpenoid quinones.

7. In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts.

8. Induction of mast-cell accumulation by promutoxin, an Arg-49 phospholipase A2.

9. Effect of yoga exercise therapy on oxidative stress indicators with end-stage renal disease on hemodialysis.

10. Effect of exercise therapy on lipid profile and oxidative stress indicators in patients with type 2 diabetes.