Xiaohui Wang1, Yang Lan2, Zhongping Zeng1, Laian Ge3. 1. Department of Gastroenterology, The Second Affiliated Hospital of Jiangxi University of TCM Nanchang, Jiangxi, China. 2. Jiangxi University of Traditional Chinese Medicine Nanchang, Jiangxi, China. 3. Department of Hepatology, The Affiliated Hospital of Jiangxi University of TCM Nanchang, Jiangxi, China.
Abstract
OBJECTIVE: This study aimed to investigate the PAR-2/TRVP1-based mechanism of steaming umbilical cord therapy with Chinese Herbal Medicine (SUCT-CHM) in IBS-D rat models. METHODS: Sixty-two IBS-D modeled rats were established, and were randomly assigned to the control group (n = 31) and the experimental group (n = 31). The model group did not receive intervention measures, and the experimental group was treated with SUCT-CHM. After 14 days of intervention, the two groups of rats were compared in terms of body weight, gastrointestinal function, Bristol stool score, wet/dry weight ratio of rat stool, and abdominal withdrawal reflex scores. The transient receptor potential vanilloid receptor 1 (TRPV1), protease-activated receptors-2 (PAR-2), calcitonin gene related peptide (CGRP) and Substance P (SP) protein expression were detected using ELISA. RESULTS: After 14 d of intervention, compared to the control group, the rats in the experimental group showed a significant increase in body mass indexes (P < 0.05); decreased Bristol stool scores (P < 0.05); less stagnation of the intestinal contents and greater intestine propulsion rate (P < 0.05), lower wet/dry weight ratio of rat stool (P < 0.05), abdominal withdrawal reflex scores (P < 0.05) as well as PAR-2, TRVP1, CGRP and SP expression levels (P < 0.05). CONCLUSION: SUCT-CHM was effective in treating IBS-D in rats. It improved gastrointestinal function and reduced visceral hypersensitivity in rats possibly via the PAR-2/TRVP1 pathway. AJTR
OBJECTIVE: This study aimed to investigate the PAR-2/TRVP1-based mechanism of steaming umbilical cord therapy with Chinese Herbal Medicine (SUCT-CHM) in IBS-D rat models. METHODS: Sixty-two IBS-D modeled rats were established, and were randomly assigned to the control group (n = 31) and the experimental group (n = 31). The model group did not receive intervention measures, and the experimental group was treated with SUCT-CHM. After 14 days of intervention, the two groups of rats were compared in terms of body weight, gastrointestinal function, Bristol stool score, wet/dry weight ratio of rat stool, and abdominal withdrawal reflex scores. The transient receptor potential vanilloid receptor 1 (TRPV1), protease-activated receptors-2 (PAR-2), calcitonin gene related peptide (CGRP) and Substance P (SP) protein expression were detected using ELISA. RESULTS: After 14 d of intervention, compared to the control group, the rats in the experimental group showed a significant increase in body mass indexes (P < 0.05); decreased Bristol stool scores (P < 0.05); less stagnation of the intestinal contents and greater intestine propulsion rate (P < 0.05), lower wet/dry weight ratio of rat stool (P < 0.05), abdominal withdrawal reflex scores (P < 0.05) as well as PAR-2, TRVP1, CGRP and SP expression levels (P < 0.05). CONCLUSION: SUCT-CHM was effective in treating IBS-D in rats. It improved gastrointestinal function and reduced visceral hypersensitivity in rats possibly via the PAR-2/TRVP1 pathway. AJTR
Authors: Nestor N Jimenez-Vargas; Luke A Pattison; Peishen Zhao; TinaMarie Lieu; Rocco Latorre; Dane D Jensen; Joel Castro; Luigi Aurelio; Giang T Le; Bernard Flynn; Carmen Klein Herenbrink; Holly R Yeatman; Laura Edgington-Mitchell; Christopher J H Porter; Michelle L Halls; Meritxell Canals; Nicholas A Veldhuis; Daniel P Poole; Peter McLean; Gareth A Hicks; Nicole Scheff; Elyssa Chen; Aditi Bhattacharya; Brian L Schmidt; Stuart M Brierley; Stephen J Vanner; Nigel W Bunnett Journal: Proc Natl Acad Sci U S A Date: 2018-07-16 Impact factor: 11.205