Ying Liang1, Gang Cheng1, Denggao Huang2, Feng Yuan1. 1. Department of Radiation Oncology, Affiliated Haikou Hospital of Xiangya Medical College, Central South University Haikou, Hainan Province, China. 2. Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University Haikou, Hainan Province, China.
Abstract
OBJECTIVE: To explore the invasion and apoptosis of head and neck squamous cell carcinoma (HNSCC) regulated by Linc00467 through the miR-1285-3p/TFAP2A axis. METHODS: qRT-PCR was used to detect the expressions of Linc00467, miR-1285-3p, and TFAP2A in tissues and cells of HNSCC patients. The targeting relationships between Linc00467 and miR-1285-3p, miR-1285-3p, and TFAP2A were verified by dual-luciferase reporter assay. Transfection and grouping were carried out, after HNSCC cell lines were screened. Transwell assay and flow cytometry were used to test cell invasion and apoptosis, respectively. RESULTS: Compared with normal tissues adjacent to the tumor, the expressions of Linc00467 and TFAP2A increased significantly in cancer tissues, while the expression of miR-1285-3p decreased (all P<0.05). Compared with the si-NC group, the invasion of the si-Linc00467 group decreased and the apoptosis rate increased (both P<0.05). In HNSCC cells, over-expression of Linc00467 promoted increased cell invasion and decreased apoptosis rate, which could be partially rescued by over-expression of miR-1285-3p (all P<0.05). Over-expression of miR-1285-3p caused decreased cell invasion and increased apoptosis rate, which was partially reversed by over-expression of TFAP2A (all P<0.05). CONCLUSION: Linc00467 can be used as ceRNA to adsorb miR-1285-3p to regulate the expression of TFAP2A, promote invasion and inhibit apoptosis of HNSCC cells. Linc00467 inhibitors may become one of the targeted therapeutic drugs for HNSCC. AJTR
OBJECTIVE: To explore the invasion and apoptosis of head and neck squamous cell carcinoma (HNSCC) regulated by Linc00467 through the miR-1285-3p/TFAP2A axis. METHODS: qRT-PCR was used to detect the expressions of Linc00467, miR-1285-3p, and TFAP2A in tissues and cells of HNSCC patients. The targeting relationships between Linc00467 and miR-1285-3p, miR-1285-3p, and TFAP2A were verified by dual-luciferase reporter assay. Transfection and grouping were carried out, after HNSCC cell lines were screened. Transwell assay and flow cytometry were used to test cell invasion and apoptosis, respectively. RESULTS: Compared with normal tissues adjacent to the tumor, the expressions of Linc00467 and TFAP2A increased significantly in cancer tissues, while the expression of miR-1285-3p decreased (all P<0.05). Compared with the si-NC group, the invasion of the si-Linc00467 group decreased and the apoptosis rate increased (both P<0.05). In HNSCC cells, over-expression of Linc00467 promoted increased cell invasion and decreased apoptosis rate, which could be partially rescued by over-expression of miR-1285-3p (all P<0.05). Over-expression of miR-1285-3p caused decreased cell invasion and increased apoptosis rate, which was partially reversed by over-expression of TFAP2A (all P<0.05). CONCLUSION:Linc00467 can be used as ceRNA to adsorb miR-1285-3p to regulate the expression of TFAP2A, promote invasion and inhibit apoptosis of HNSCC cells. Linc00467 inhibitors may become one of the targeted therapeutic drugs for HNSCC. AJTR
Authors: Kartik Vasan; Tsu-Hui Hubert Low; Ruta Gupta; Bruce Ashford; Rebecca Asher; Kan Gao; Sydney Ch'ng; Carsten E Palme; Jonathan R Clark Journal: Head Neck Date: 2018-01-23 Impact factor: 3.147