BACKGROUND: The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil. METHODS: In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval. RESULTS: In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76-70.56) than against DENV (95.05% with 95% CI: 78.09-98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29-34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01-5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89-4.56). CONCLUSION: The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.
BACKGROUND: The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil. METHODS: In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval. RESULTS: In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76-70.56) than against DENV (95.05% with 95% CI: 78.09-98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29-34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01-5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89-4.56). CONCLUSION: The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.
Authors: José Henrique Francisco Roma; Rachel Cruz Alves; Vanessa Siano da Silva; Marcio José Ferreira; Claudinéia de Araújo; Juliana Helena Chavéz Pavoni Journal: Rev Soc Bras Med Trop Date: 2019-07-18 Impact factor: 1.581
Authors: Marcio Roberto Teixeira Nunes; Nuno Rodrigues Faria; Janaina Mota de Vasconcelos; Nick Golding; Moritz U G Kraemer; Layanna Freitas de Oliveira; Raimunda do Socorro da Silva Azevedo; Daisy Elaine Andrade da Silva; Eliana Vieira Pinto da Silva; Sandro Patroca da Silva; Valéria Lima Carvalho; Giovanini Evelim Coelho; Ana Cecília Ribeiro Cruz; Sueli Guerreiro Rodrigues; Joao Lídio da Silva Gonçalves Vianez; Bruno Tardelli Diniz Nunes; Jedson Ferreira Cardoso; Robert B Tesh; Simon I Hay; Oliver G Pybus; Pedro Fernando da Costa Vasconcelos Journal: BMC Med Date: 2015-04-30 Impact factor: 8.775
Authors: Vittorio Sambri; Maria R Capobianchi; Francesca Cavrini; Rémi Charrel; Olivier Donoso-Mantke; Camille Escadafal; Leticia Franco; Paolo Gaibani; Ernest A Gould; Matthias Niedrig; Anna Papa; Anna Pierro; Giada Rossini; Andrea Sanchini; Antonio Tenorio; Stefania Varani; Ana Vázquez; Caterina Vocale; Herve Zeller Journal: Viruses Date: 2013-09-25 Impact factor: 5.048
Authors: Susannah Colt; Maria N Garcia-Casal; Juan Pablo Peña-Rosas; Julia L Finkelstein; Pura Rayco-Solon; Zita C Weise Prinzo; Saurabh Mehta Journal: PLoS Negl Trop Dis Date: 2017-04-10
Authors: Chantal B F Vogels; Claudia Rückert; Sean M Cavany; T Alex Perkins; Gregory D Ebel; Nathan D Grubaugh Journal: PLoS Biol Date: 2019-01-22 Impact factor: 8.029