Literature DB >> 34302650

Generation of Cylindrical Engineered Cardiac Tissues from Human iPS Cell-Derived Cardiovascular Cell Lineages.

Hidetoshi Masumoto1,2.   

Abstract

The present protocol describes a method to generate cylindrical engineered cardiac tissues (ECTs) composed of cardiovascular cell lineages induced from human induced pluripotent stem cells (hiPSCs). Cardiomyocytes, endothelial cells, and vascular mural cells induced from hiPSCs are mixed with gel matrix and poured into a tissue mold with posts. By culture day 14, the mixed culture matures into a cylindrical ECT which beats spontaneously and synchronously. Cardiomyocytes align to the long axis of the ECT. The ECTs generated by the present method may be regarded as a surrogate of human myocardium and be served as researches in cardiac regenerative medicine, disease modeling, drug discovery, and cardiac toxicity tests.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cardiac differentiation; Cell culture; Engineered cardiac tissue; Induced pluripotent stem cell; Tissue engineering

Year:  2021        PMID： 34302650     DOI： 10.1007/978-1-0716-1484-6_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  Cell therapy for heart failure: a comprehensive overview of experimental and clinical studies, current challenges, and future directions.

Authors:  Santosh K Sanganalmath; Roberto Bolli
Journal:  Circ Res       Date:  2013-08-30       Impact factor: 17.367

Review 2.  Cardiac cell therapy: Current status, challenges and perspectives.

Authors:  Philippe Menasché
Journal:  Arch Cardiovasc Dis       Date:  2020-03-11       Impact factor: 2.340

Review 3.  Development of torsadogenic risk assessment using human induced pluripotent stem cell-derived cardiomyocytes: Japan iPS Cardiac Safety Assessment (JiCSA) update.

Authors:  Yasunari Kanda; Daiju Yamazaki; Tomoharu Osada; Takashi Yoshinaga; Kohei Sawada
Journal:  J Pharmacol Sci       Date:  2018-10-24       Impact factor: 3.337

4.  CSAHi study: Evaluation of multi-electrode array in combination with human iPS cell-derived cardiomyocytes to predict drug-induced QT prolongation and arrhythmia--effects of 7 reference compounds at 10 facilities.

Authors:  Takashi Kitaguchi; Yuta Moriyama; Tomohiko Taniguchi; Atsuko Ojima; Hiroyuki Ando; Takaaki Uda; Koji Otabe; Masao Oguchi; Shigekazu Shimizu; Hiroyuki Saito; Maya Morita; Atsushi Toratani; Mahoko Asayama; Wataru Yamamoto; Emi Matsumoto; Daisuke Saji; Hiroki Ohnaka; Kohji Tanaka; Ikumi Washio; Norimasa Miyamoto
Journal:  J Pharmacol Toxicol Methods       Date:  2015-12-04       Impact factor: 1.950

5.  Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration.

Authors:  Hidetoshi Masumoto; Takeshi Ikuno; Masafumi Takeda; Hiroyuki Fukushima; Akira Marui; Shiori Katayama; Tatsuya Shimizu; Tadashi Ikeda; Teruo Okano; Ryuzo Sakata; Jun K Yamashita
Journal:  Sci Rep       Date:  2014-10-22       Impact factor: 4.379

6.  The myocardial regenerative potential of three-dimensional engineered cardiac tissues composed of multiple human iPS cell-derived cardiovascular cell lineages.

Authors:  Hidetoshi Masumoto; Takeichiro Nakane; Joseph P Tinney; Fangping Yuan; Fei Ye; William J Kowalski; Kenji Minakata; Ryuzo Sakata; Jun K Yamashita; Bradley B Keller
Journal:  Sci Rep       Date:  2016-07-20       Impact factor: 4.379

7.  Impact of Cell Composition and Geometry on Human Induced Pluripotent Stem Cells-Derived Engineered Cardiac Tissue.

Authors:  Takeichiro Nakane; Hidetoshi Masumoto; Joseph P Tinney; Fangping Yuan; William J Kowalski; Fei Ye; Amanda J LeBlanc; Ryuzo Sakata; Jun K Yamashita; Bradley B Keller
Journal:  Sci Rep       Date:  2017-04-03       Impact factor: 4.379

8.  Modelling Torsade de Pointes arrhythmias in vitro in 3D human iPS cell-engineered heart tissue.

Authors:  Masahide Kawatou; Hidetoshi Masumoto; Hiroyuki Fukushima; Gaku Morinaga; Ryuzo Sakata; Takashi Ashihara; Jun K Yamashita
Journal:  Nat Commun       Date:  2017-10-20       Impact factor: 14.919
  8 in total
  1 in total

Review 1.  Application of the Pluripotent Stem Cells and Genomics in Cardiovascular Research-What We Have Learnt and Not Learnt until Now.

Authors:  Michael Simeon; Seema Dangwal; Agapios Sachinidis; Michael Xavier Doss
Journal:  Cells       Date:  2021-11-10       Impact factor: 6.600
  1 in total

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