Salim Levent Aktug1, Salih Durdu2, Selin Kalkan3, Kultigin Cavusoglu4, Metin Usta5,6. 1. Materials Science and Engineering, Gebze Technical University, 41400, Gebze, Turkey. 2. Industrial Engineering, Giresun University, 28200, Giresun, Turkey. 3. Bioprocess Engineering, Giresun University, 28200, Giresun, Turkey. 4. Department of Biology, Giresun University, 28200, Giresun, Turkey. 5. Materials Science and Engineering, Gebze Technical University, 41400, Gebze, Turkey. ustam@gtu.edu.tr. 6. Materials Institute, TUBITAK Marmara Research Center, 41470, Gebze, Turkey. ustam@gtu.edu.tr.
Abstract
Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO2, metastable Ca0.15Zr0.85O1.85, and Ca3(PO4)2 were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.
Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (pan class="Chemical">MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO2, metastable Ca0.15Zr0.85O1.85, and Ca3(PO4)2 were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.
Authors: Chiara Battocchio; Sofia Concolato; Serena De Santis; Mats Fahlman; Giovanna Iucci; Marta Santi; Giovanni Sotgiu; Monica Orsini Journal: Mater Sci Eng C Mater Biol Appl Date: 2019-02-15 Impact factor: 7.328
Authors: S Fidan; F Muhaffel; M Riool; G Cempura; L de Boer; S A J Zaat; A Czyrska- Filemonowicz; H Cimenoglu Journal: Mater Sci Eng C Mater Biol Appl Date: 2016-11-10 Impact factor: 7.328