Qingyao Wang1,2, Yixiang Zhang3,4, Qian Yang5, Songzhe Fu6,7, Baocheng Qu1,2, Tom Defoirdt5. 1. College of Marine Science and Environment, Dalian Ocean University, No. 52 Heishijiao Street, Dalian, 116023, China. 2. Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, 116023, Dalian, China. 3. CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, China. 4. University of Chinese Academy of Sciences, Shanghai, China. 5. Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium. 6. College of Marine Science and Environment, Dalian Ocean University, No. 52 Heishijiao Street, Dalian, 116023, China. fusongzhe@dlou.edu.cn. 7. Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, 116023, Dalian, China. fusongzhe@dlou.edu.cn.
Abstract
BACKGROUND: Intensive aquaculture farming has caused significant degradation of coastal wetlands and has been proposed as a reservoir for pathogenic Vibrio spp. RESULTS: Gut pathogens including Vibrio spp., Salmonella spp., and Klebsiella spp. were isolated from bird feces, shrimp and wetland water in two typical coastal regions of China in 2015 and 2017 and were subsequently subjected to whole-genome sequencing. Meanwhile, local patient isolates were also selected to confirm the epidemiological links. Bacterial community composition analyses of the sediments that were sampled in 2015 and 2017 were conducted by the hypervariable region 4 of the 16S rRNA gene. Together with the local clinical isolates, we observed highly related Vibrio isolates from waterbirds, wetlands and shrimp. Phylogenetic genome comparisons also demonstrated that sequence types ST3 and ST2414 Vibrio parahaemolyticus isolates obtained from aquatic animals were clonally related to patient isolates. Likewise, three Salmonella typhimurium isolates were also genomically related to one clinical strain. The results showed that farming activities significantly altered the community composition and resulted in the emergence of several pathogens, including Acinetobacter, Mycobacterium and Legionella. CONCLUSIONS: In conclusion, our results demonstrated that intensive shrimp farming in wetlands has two devastating impacts: pathogen dissemination from aquatic animals into migratory birds and transmission of foodborne pathogens into local communities.
BACKGROUND: Intensive aquaculture farming has caused significant degradation of coastal wetlands and has been proposed as a reservoir for pathogenic Vibrio spp. RESULTS: Gut pathogens including Vibrio spp., Salmonella spp., and Klebsiellaspp. were isolated from bird feces, shrimp and wetland water in two typical coastal regions of China in 2015 and 2017 and were subsequently subjected to whole-genome sequencing. Meanwhile, local patient isolates were also selected to confirm the epidemiological links. Bacterial community composition analyses of the sediments that were sampled in 2015 and 2017 were conducted by the hypervariable region 4 of the 16S rRNA gene. Together with the local clinical isolates, we observed highly related Vibrio isolates from waterbirds, wetlands and shrimp. Phylogenetic genome comparisons also demonstrated that sequence types ST3 and ST2414 Vibrio parahaemolyticus isolates obtained from aquatic animals were clonally related to patient isolates. Likewise, three Salmonella typhimurium isolates were also genomically related to one clinical strain. The results showed that farming activities significantly altered the community composition and resulted in the emergence of several pathogens, including Acinetobacter, Mycobacterium and Legionella. CONCLUSIONS: In conclusion, our results demonstrated that intensive shrimp farming in wetlands has two devastating impacts: pathogen dissemination from aquatic animals into migratory birds and transmission of foodborne pathogens into local communities.
Authors: Andrew M Ray; Adam J Sepulveda; Kathryn M Irvine; Siri K C Wilmoth; David P Thoma; Debra A Patla Journal: Sci Total Environ Date: 2019-02-21 Impact factor: 7.963
Authors: Luigi Vezzulli; Chiara Grande; Philip C Reid; Pierre Hélaouët; Martin Edwards; Manfred G Höfle; Ingrid Brettar; Rita R Colwell; Carla Pruzzo Journal: Proc Natl Acad Sci U S A Date: 2016-08-08 Impact factor: 11.205
Authors: Jonathan A Patz; Peter Daszak; Gary M Tabor; A Alonso Aguirre; Mary Pearl; Jon Epstein; Nathan D Wolfe; A Marm Kilpatrick; Johannes Foufopoulos; David Molyneux; David J Bradley Journal: Environ Health Perspect Date: 2004-07 Impact factor: 9.031