Performance of formalin ethyl acetate, percoll sedimentation and ELISA for the detection of Cryptosporidium infection in asymptomatic children.

This study aimed to assess the performance of formalin ethyl acetate (FEA)/modified Ziehl-Neelsen (MZN), and percoll technique/MZN for the diagnosis of cryptosporidiosis among asymptomatic children compared to ELISA coproantigen. The study was conducted on 100 children in a rural area in Kafr El-Sheikh governorate. Stool samples were collected and examined by the three techniques. Microscopic examination revealed the presence of acid-fast stained oocysts and non-acid fast ghost oocysts. The overall prevalence rate was 7% with an infection intensity of 1-5 oocysts/oil immersion field. FEA/MZN technique showed the highest diagnostic performance (5%) with 71.4% sensitivity and 98% negative predictive value (NPV) compared to the other techniques. ELISA revealed 3% prevalence, 42.9% sensitivity and 96% NPV. Percoll/MZN gave the lowest prevalence, sensitivity and NPV (1%, 14.29% and 93.9% respectively). Agreement fluctuated between moderate and poor regarding FEA/MZN versus ELISA and percoll/MZN versus both techniques. In conclusion, FEA/MZN gave the top diagnostic performance, yet it missed some positive cases. Its combination with ELISA coproantigen might prove beneficial for Cryptosporidium diagnosis. Percoll technique needs more validation by modifying the density gradient, speed of centrifugation, and staining methods. © Indian Society for Parasitology 2021.