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Literature DB >> 34292542

Study of Fe-S Cluster Proteins in Methanococcus maripaludis , a Model Archaeal Organism.

Cuiping Zhao¹, Christina A Roberts², Ian J Drake³, Yuchen Liu⁴.

Abstract

Iron-sulfur (Fe-S) clusters are among the oldest and most versatile cofactors present in all domains of life. Many bacterial and eukaryotic Fe-S proteins have been well-characterized, whereas the archaeal ones are less studied. Fe-S proteins are particularly abundant and play essential roles in methanogenic archaea. Methanococcus maripaludis is a model methanogen with available genetic tools. Here, we describe the techniques for anaerobic cultivation of M. maripaludis with formate, liposome-mediated transformation, expression and anoxic affinity purification of Fe-S proteins, Fe-S cluster reconstitution, and analysis of Fe-S proteins by UV-visible absorption spectroscopy.

Entities: Chemical

Keywords: Anaerobic cultivation; Anoxic protein purification; Archaea; Fe-S cluster reconstitution; Iron-sulfur clusters; Liposome-mediated transformation; Methanococcus; Methanogen; UV-visible absorption spectroscopy

Mesh：

Substances：

Year: 2021 PMID： 34292542 DOI： 10.1007/978-1-0716-1605-5_2

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

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Study of Fe-S Cluster Proteins in Methanococcus maripaludis , a Model Archaeal Organism.

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Review 2. Iron-sulphur clusters and the problem with oxygen.

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