Literature DB >> 34286044

Functional Evaluation of the Signal Peptides of Secreted Proteins.

Weixiao Yin1, Yufu Wang1, Tao Chen1, Yang Lin1, Chaoxi Luo1.   

Abstract

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Function; Secreted protein; Signal peptide; Yeast; pSUC2 vector

Year:  2018        PMID： 34286044      PMCID： PMC8275294          DOI： 10.21769/BioProtoc.2839

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  SignalP 4.0: discriminating signal peptides from transmembrane regions.

Authors:  Thomas Nordahl Petersen; Søren Brunak; Gunnar von Heijne; Henrik Nielsen
Journal:  Nat Methods       Date:  2011-09-29       Impact factor: 28.547

2.  A genetic selection for isolating cDNAs encoding secreted proteins.

Authors:  K A Jacobs; L A Collins-Racie; M Colbert; M Duckett; M Golden-Fleet; K Kelleher; R Kriz; E R LaVallie; D Merberg; V Spaulding; J Stover; M J Williamson; J M McCoy
Journal:  Gene       Date:  1997-10-01       Impact factor: 3.688

3.  Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

Authors:  R Daniel Gietz; Robert H Schiestl
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

4.  RXLR-mediated entry of Phytophthora sojae effector Avr1b into soybean cells does not require pathogen-encoded machinery.

Authors:  Daolong Dou; Shiv D Kale; Xia Wang; Rays H Y Jiang; Nathan A Bruce; Felipe D Arredondo; Xuemin Zhang; Brett M Tyler
Journal:  Plant Cell       Date:  2008-07-11       Impact factor: 11.277

5.  In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2.

Authors:  Sang-Keun Oh; Carolyn Young; Minkyoung Lee; Ricardo Oliva; Tolga O Bozkurt; Liliana M Cano; Joe Win; Jorunn I B Bos; Hsin-Yin Liu; Mireille van Damme; William Morgan; Doil Choi; Edwin A G Van der Vossen; Vivianne G A A Vleeshouwers; Sophien Kamoun
Journal:  Plant Cell       Date:  2009-09-30       Impact factor: 11.277

6.  An Oomycete CRN Effector Reprograms Expression of Plant HSP Genes by Targeting their Promoters.

Authors:  Tianqiao Song; Zhenchuan Ma; Danyu Shen; Qi Li; Wanlin Li; Liming Su; Tingyue Ye; Meixiang Zhang; Yuanchao Wang; Daolong Dou
Journal:  PLoS Pathog       Date:  2015-12-29       Impact factor: 6.823
  6 in total
  8 in total

1.  A serine-rich effector from the stripe rust pathogen targets a Raf-like kinase to suppress host immunity.

Authors:  Cuiping Wan; Yan Liu; Shuxin Tian; Jia Guo; Xingxuan Bai; Haochuan Zhu; Zhensheng Kang; Jun Guo
Journal:  Plant Physiol       Date:  2022-08-29       Impact factor: 8.005

2.  A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response.

Authors:  Feng Xiao; Wenxing Xu; Ni Hong; Liping Wang; Yongle Zhang; Guoping Wang
Journal:  Int J Mol Sci       Date:  2022-05-28       Impact factor: 6.208

3.  Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae.

Authors:  Na Ning; Xin Xie; Haiyue Yu; Jie Mei; Qianqian Li; Shimin Zuo; Hanxiang Wu; Wende Liu; Zhiqiang Li
Journal:  Int J Mol Sci       Date:  2022-02-24       Impact factor: 5.923

4.  Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of Colletotrichum gloeosporioides in Apple Leaf.

Authors:  Meiyu Wang; Zhirui Ji; Haifeng Yan; Jie Xu; Xuanzhu Zhao; Zongshan Zhou
Journal:  Int J Mol Sci       Date:  2022-06-07       Impact factor: 6.208

5.  Transcriptome Analysis of Fusarium-Tomato Interaction Based on an Updated Genome Annotation of Fusarium oxysporum f. sp. lycopersici Identifies Novel Effector Candidates That Suppress or Induce Cell Death in Nicotiana benthamiana.

Authors:  Xizhe Sun; Xiangling Fang; Dongmei Wang; David A Jones; Lisong Ma
Journal:  J Fungi (Basel)       Date:  2022-06-26

6.  Glycine-serine-rich effector PstGSRE4 in Puccinia striiformis f. sp. tritici inhibits the activity of copper zinc superoxide dismutase to modulate immunity in wheat.

Authors:  Cong Liu; Yunqian Wang; Yanfeng Wang; Yuanyuan Du; Chao Song; Ping Song; Qian Yang; Fuxin He; Xingxuan Bai; Lili Huang; Jia Guo; Zhensheng Kang; Jun Guo
Journal:  PLoS Pathog       Date:  2022-07-26       Impact factor: 7.464

7.  Phytophthora capsici CBM1-containing protein CBP3 is an apoplastic effector with plant immunity-inducing activity.

Authors:  Zhiyuan Yin; Nan Wang; Weiwei Duan; Lei Pi; Danyu Shen; Daolong Dou
Journal:  Mol Plant Pathol       Date:  2021-08-11       Impact factor: 5.663

8.  Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death.

Authors:  Lisen Liu; Zhaohan Wang; Jianing Li; Ye Wang; Jiachen Yuan; Jingjing Zhan; Peng Wang; Yongjun Lin; Fuguang Li; Xiaoyang Ge
Journal:  Mol Plant Pathol       Date:  2021-07-07       Impact factor: 5.663
  8 in total

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