Literature DB >> 17401336

Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

R Daniel Gietz1, Robert H Schiestl.   

Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

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Year:  2007        PMID： 17401336     DOI： 10.1038/nprot.2007.15

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  110 in total

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Authors:  Jian Li; Kristen Kolberg; Ulrich Schlecht; Robert P St Onge; Ana Maria Aparicio; Joe Horecka; Ronald W Davis; Maureen E Hillenmeyer; Colin J B Harvey
Journal:  J Biol Chem       Date:  2018-12-04       Impact factor: 5.157

2.  Fitness analyses of all possible point mutations for regions of genes in yeast.

Authors:  Ryan Hietpas; Benjamin Roscoe; Li Jiang; Daniel N A Bolon
Journal:  Nat Protoc       Date:  2012-06-21       Impact factor: 13.491

3.  Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.

Authors:  Laura M Walker; Diana R Bowley; Dennis R Burton
Journal:  J Mol Biol       Date:  2009-04-16       Impact factor: 5.469

4.  High-throughput analysis of in vivo protein stability.

Authors:  Ikjin Kim; Christina R Miller; David L Young; Stanley Fields
Journal:  Mol Cell Proteomics       Date:  2013-07-29       Impact factor: 5.911

5.  Mismatch Repair Incompatibilities in Diverse Yeast Populations.

Authors:  Duyen T Bui; Anne Friedrich; Najla Al-Sweel; Gianni Liti; Joseph Schacherer; Charles F Aquadro; Eric Alani
Journal:  Genetics       Date:  2017-02-13       Impact factor: 4.562

6.  A sequence-specific interaction between the Saccharomyces cerevisiae rRNA gene repeats and a locus encoding an RNA polymerase I subunit affects ribosomal DNA stability.

Authors:  Inswasti Cahyani; Andrew G Cridge; David R Engelke; Austen R D Ganley; Justin M O'Sullivan
Journal:  Mol Cell Biol       Date:  2014-11-24       Impact factor: 4.272

7.  Improved bioethanol production using CRISPR/Cas9 to disrupt the ADH2 gene in Saccharomyces cerevisiae.

Authors:  Ting Xue; Kui Liu; Duo Chen; Xue Yuan; Jingping Fang; Hansong Yan; Luqiang Huang; Youqiang Chen; Wenjin He
Journal:  World J Microbiol Biotechnol       Date:  2018-10-01       Impact factor: 3.312

8.  A competing hydrophobic tug on L596 to the membrane core unlatches S4-S5 linker elbow from TRP helix and allows TRPV4 channel to open.

Authors:  Jinfeng Teng; Stephen H Loukin; Andriy Anishkin; Ching Kung
Journal:  Proc Natl Acad Sci U S A       Date:  2016-10-03       Impact factor: 11.205

9.  Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties.

Authors:  Sarah K Baxter; Abigail R Lambert; Andrew M Scharenberg; Jordan Jarjour
Journal:  Methods Mol Biol       Date:  2013

10.  High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display.

Authors:  Jordan Jarjour; Hoku West-Foyle; Michael T Certo; Christopher G Hubert; Lindsey Doyle; Melissa M Getz; Barry L Stoddard; Andrew M Scharenberg
Journal:  Nucleic Acids Res       Date:  2009-09-08       Impact factor: 16.971
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