Pia Stockinger1, Andreas Berlin1, Daniel Kampik1, Christine Schmitt1, Jost Hillenkamp1, Jeffrey D Messinger2, Martina C Herwig-Carl3, Thomas Ach4,5. 1. Department of Ophthalmology, University Hospital Würzburg, Würzburg, Germany. 2. Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, AL, USA. 3. Department of Ophthalmology, University Hospital Bonn, Ernst Abbe-Str. 2, 53127, Bonn, Germany. 4. Department of Ophthalmology, University Hospital Würzburg, Würzburg, Germany. thomas.ach@ukbonn.de. 5. Department of Ophthalmology, University Hospital Bonn, Ernst Abbe-Str. 2, 53127, Bonn, Germany. thomas.ach@ukbonn.de.
Abstract
BACKGROUND: For an understanding of the pathology of retinal diseases, direct comparisons of high-resolution in vivo retinal imaging and ex vivo histological preparations are desirable. MATERIAL AND METHODS: Multimodal in vivo and ex vivo imaging of a human donor eye with secondary alterations showing atrophic retina due to central retinal arterial occlusion. The subsequent correlation with the histological examination was carried out on identical tissue localizations. RESULTS: Appropriate custom-built retinal imaging devices facilitate in vivo and ex vivo correlations and the examination of human eye tissue and acquisition of retinal images, e.g. SD-OCT. The precise alignment of the tissue enables a histological analysis on identical sites. CONCLUSION: The direct correlation of clinical in vivo imaging with ex vivo imaging including histopathology can further enhance our understanding in the pathogenesis of retinal diseases; however, the proposed method is currently limited due to restricted availability of human donor tissue.
BACKGROUND: For an understanding of the pathology of retinal diseases, direct comparisons of high-resolution in vivo retinal imaging and ex vivo histological preparations are desirable. MATERIAL AND METHODS: Multimodal in vivo and ex vivo imaging of a human donor eye with secondary alterations showing atrophic retina due to central retinal arterial occlusion. The subsequent correlation with the histological examination was carried out on identical tissue localizations. RESULTS: Appropriate custom-built retinal imaging devices facilitate in vivo and ex vivo correlations and the examination of human eye tissue and acquisition of retinal images, e.g. SD-OCT. The precise alignment of the tissue enables a histological analysis on identical sites. CONCLUSION: The direct correlation of clinical in vivo imaging with ex vivo imaging including histopathology can further enhance our understanding in the pathogenesis of retinal diseases; however, the proposed method is currently limited due to restricted availability of human donor tissue.