| Literature DB >> 34251558 |
Shinya Sasaki1, Katsuhiro Takeda2, Kazuhisa Ouhara1, Satomi Shirawachi3, Mikihito Kajiya1, Shinji Matsuda1, Shoko Kono1, Hideki Shiba3, Hidemi Kurihara1, Noriyoshi Mizuno1.
Abstract
Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration. Tissue regeneration is characterized by inflammation, which directs the quality of tissue repair. This study aimed to investigate the effect of BDNF on the phagocytic activity of RAW264.7 cells. In addition, we studied the effect of BDNF on guanosine triphosphatase (GTP)-RAS-related C3 botulinus toxin substrate (Rac)1 and phospho-Rac1 levels in RAW264.7 cells. Rac1 inhibitor inhibited BDNF-induced phagocytosis of latex-beads. In addition, BDNF enhanced Porphyromonas gingivalis (Pg) phagocytosis by RAW264.7 cells as well as latex-beads. We demonstrated for the first time that BDNF enhances phagocytic activity of RAW264.7 cells through Rac1 activation. The present study proposes that BDNF may reduce inflammatory stimuli during BDNF-induced periodontal tissue regeneration through enhanced phagocytic activity of macrophages.Entities:
Keywords: Brain-derived neurotrophic factor; Macrophage; Phagocytosis; Rac1
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Year: 2021 PMID： 34251558 DOI： 10.1007/s11033-021-06531-6
Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316