Antioxidant and Antimicrobial Activities of the Extracts from Different Garcinia Species.

BACKGROUND: Garcinia is a large genus which has promising bioactivities. However, the properties of many Garcinia species have not been investigated thoroughly. AIM: To determine the antioxidant and antimicrobial capabilities of the extracts from different Garcinia species. Methodology. Six Garcinia species, including Garcinia fusca, Garcinia hopii, Garcinia planchonii, Garcinia nigrolineata, Garcinia gaudichaudii, and Garcinia tinctoria were extracted using n-hexane, ethyl acetate, and methanol, producing n-hexane extract (HE), ethyl acetate extract (EAE), and methanol extract (ME). After that, the total polyphenol content was evaluated using Folin-Ciocalteu assay. DPPH, hydroxyl radical scavenging, and total antioxidant capacity assays were performed to test the antioxidant activity. Subsequently, the antimicrobial activities against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains were assessed using Kirby Bauer and the broth microdilution methods.
RESULTS: Many Garcinia extracts contained high total polyphenol content consisting of ME of G. hopii ad G. tinctoria, and EAE of G. planchonii and G. tinctoria. The EAE of G. tinctoria showed effective antioxidant capacity (IC50 = 1.5 µg/mL). Additionally, the EAE of G. gaudichaudii was effective against Gram-positive bacteria with minimal inhibition concentration (MIC) of 15.625-25 µg/mL whereas ME of G. planchonii was effective against both Gram-positive bacteria (MIC = 160 µg/mL) and Gram-negative bacteria (MIC = 75 µg/mL).
CONCLUSION: Several extracts of Garcinia species demonstrated valuable antioxidant and antimicrobial properties.

1. Introduction

The genus Garcinia belonging to the Clusiaceae family is a sizable group of plants. It consists of over 200 species mainly distributing in the world's tropics, chiefly in Asia, Africa, and Polynesia. They are evergreen polygamous trees, shrubs, and herbs. Many species of Garcinia have been proven highly nutritious and utilized as food, supplemental products, and nutritional supplements. The most widely studied is G. mangostana or mangosteen, the extract of which contains a high amount of isoprenylated xanthones exerting anticancer, analgesic, neuroprotective, antidiabetic, and hypolipidemic effects [1]. Another species of Garcinia is G. cambogia, whose extract containing high hydroxycitric acid which is used as a weight-loss supplement by reducing fat accumulation [2, 3]. Moreover, the supplement of G. cambogia also helps to reduce inflammation and glucose intolerance [4]. Other species of Garcinia have also been investigated; those include G. celebica, G. nigrolineata, G. cowa, G. indica, G. schomburgkiana Pierre., G. preussii, and G. fusca [1, 5–8]. Specifically, the extract G. celebica leaves exerted anticancer activities by enhancing apoptosis on MCF-7 human breast cancer cell lines [5]. In addition, the leaf extract of G. celebica and extracted compound, catechin, exhibited antiproliferation on Plasmodium falciparum by oxidative stress induction [9]. Likewise, xanthones collected from the root of G. fusca inhibited α-glucosidase, making it a candidate for antidiabetes [10] whereas quinone and xanthones isolated from the extracts of G. nigrolineata show high antibacterial activity [6, 11, 12]. Another outstanding feature is that the fruit rind of G. indica possesses both antioxidant and hepatoprotective properties [13].

In modern societies, the increasing level of stress, air pollution, food toxicity, and so forth causes reactive oxygen species production, leading to DNA damage, which gives rise to several chronic and degenerative diseases such as antiaging, anti-inflammatory, antiatherosclerosis, and anticancer [14, 15]. Antioxidants are substances that inhibit the initiation or propagation of oxidative chain reactions, scavenge free radicals, quench singlet oxygen, and reducing agents, resulting in the reduction of oxidative stress for the cell [16, 17]. Therefore, finding substances and extracts with high antioxidant capacity is a crucial issue.

The plan is believed to be a crucial source of natural antioxiadants and antimicrobial activity. Firstly, these natural antioxidants, especially polyphenols and carotenoids, are capable of scavenging free radicals or stabilizing free reactive oxygen species to prevent oxidative stress within the cell environment. Studies demonstrated that several Garcinia species possessed the high antioxidant ability, including G. mangostana L., G. indica, G. cambogia, G. atroviridis [13, 18–21]. The antioxidant activity of these extracts has been applied to treat various related diseases. Secondly, with regard to antimicrobial activity, the plant extracts might be derived from phenolics, terpenoids, essential oil, alkaloids, lectins, polypeptides, and polyacetylenes [22-24]. Several Garcinia has demonstrated the capability of exhibiting antimicrobial activity. For instance, the extracts of G. cambogia leaves and G. indica fruits showed interesting anthelmintic and antibacterial activities against earthworm and Gram-positive bacteria, respectively [25, 26].

In Vietnam, there are several Garcinia species grown wildly, of which their bioactivities have not been clearly elucidated. In this study, six Garcinia species including G. fusca, G. hopii, G. planchonii, G. nigrolineata, G. gaudichaudii, and G. tinctoria were collected and extracted using different solvents. Subsequently, the polyphenolic content, the antioxidation, and the antimicrobial capability of the extracts were examined.

2. Materials and Methods

2.1. Materials

The plant samples were collected from different Vietnam provinces and identified by Dr. Son V. Dang, the curator of the VNM Herbarium, Institute of Tropical Biology, Ho Chi Minh City, Vietnam. Voucher specimens were deposited in the Natural Product and Medicinal Chemistry Lab, University of Science, VNU-HCM. Data of sample collection are shown in Table 1.

Table 1

Summarization of sample collection and extract preparation of various species of genus Garcinia.

Garcinia species	Collecting part	Collecting region	Collecting date	Weight of extracts (g)
G. fusca	Roots	Dong Nai	March 2015	Hexane	2.90
				Ethyl acetate	6.67
				Methanol	4.97
G. hopii	Stems	Khanh Hoa	April 2015	Hexane	0.64
	Leaves			Ethyl acetate	0.97
				Methanol	0.82
G. planchonii	Barks	Khanh Hoa	April 2015	Hexane	10.19
				Ethyl acetate	9.00
				Methanol	5.61
G. nigrolineata	Barks	Dong Nai	March 2016	Hexane	7.33
				Ethyl acetate	1.32
				Methanol	3.37
G. gaudichaudii	Barks	Phu Quoc	May 2016	Hexane	4.86
				Ethyl acetate	0.78
				Methanol	2.21
G. tinctoria	Barks	Dong Nai	May 2016	Hexane	9.26
				Ethyl acetate	3.61
				Methanol	3.35

Solvents such as n-hexane, ethyl acetate, methanol, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO, USA). Folin–Ciocalteu and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma-Aldrich (St Louis, MO, USA). Mueller-Hinton Broth and Mueller-Hinton Agar were acquired from Thermo Fisher Scientific (Massachusetts, USA).

2.2. Methods

2.2.1. The Preparation of Garcinia Extracts

One hundred grams of each plant material was air-dried, grounded, and then subjected to Soxhlet extraction with 350 mL of methanol to yield a concentrated solution. Methanol was then removed under reduced pressure using a rotary evaporator system to obtain an initial extract. The extract was then subjected to solid-phase extraction, where the sample was thoroughly washed with 5 L of n-hexane, 10 L of ethyl acetate, and 10 L of methanol, respectively. After concentration, n-hexane extract (HE), ethyl acetate extract (EAE), and methanol extract (ME) were attained. Data of extract preparation are described in Table 1.

2.2.2. The Measurement of Total Phenolic Content

Total phenolic content (TPC) of Garcinia extracts was determined using Folin–Ciocalteu assay, following the protocol of Singleton et al. [27]. In particular, 800 µL of HE, EAE, and ME at the concentration of 50 μg/mL were mixed with 100 μL of Folin–Ciocalteu reagent (diluted 4 times), followed by an addition of 100 μL of 20% (w/v) sodium carbonate. After being mixed carefully, the mixture was kept in the dark at room temperature for 30 mins. The absorbance of these samples was measured at 760 nm using a spectrophotometer (Thermo Scientific, Massachusetts, USA). Gallic acid was used as a reference. Gallic acid at different concentrations (200, 100, 50, 25, 12.5, 6.25, 3.125, and 1.56 μg/mL) was applied following the aforementioned protocol to prepare a standard curve. TPC was expressed as milligrams of gallic acid equivalent (GAE) per gram of the dry extract.

2.2.3. DPPH Assay

The free radical scavenging activity of the extracts was evaluated using DPPH assay according to the method described by Shen et al. [28]. The extracts were diluted in 0.1 mM DMSO to various concentrations from 0 to 200 μg/mL. 0.5 mL of the extracted was added to 1.5 mL of 0.1 mM DPPH which was dissolved completely in methanol. The mixtures were shaken vigorously and incubated in the dark at room temperature for 30 mins. Then, the absorbance was measured at 517 nm using a spectrophotometer. Ascorbic acid was used as a positive control. The capability of scavenging the DPPH radical was calculated using the following formula:

A 0 is the absorbance of the control reaction and A1 is the absorbance in the presence of the extracts or positive control.

2.2.4. Determination of Hydroxyl Radical Scavenging Assay

The hydroxyl radical scavenging activity of the extracts was assessed according to the protocol of Klein et al. [29]. 90 μL of the extracts with the concentrations ranging from 0 to 1,000 μg/mL was supplemented with 45 μL of 8 mM FeSO4.7H2O, 63 μL of 5.7 mM salicylic acid, and 72 μL of 6 mM H2O2. The mixture was incubated at 37°C for 30 mins before being applied onto a spectrophotometer to measure the absorbance at 562 nm. Ascorbic acid was used as a positive control. The percentage of the hydroxyl scavenging activity was calculated using the following formula:

A sample blank or Acontrol blank is the absorbance value of sample or control without salicylic acid.

A sample or Acontrol is the absorbance value of the sample or control with salicylic acid.

2.2.5. Determination of Total Antioxidant

The total antioxidant capacity (TAC) of the extracts was estimated following the method reported by Wan C. et al. [30]. 0.1 mL of the extracts at the concentration of 200 μg/mL was added with 0.9 mL of 0.6 mol/L sulfuric acid, 28 mmol/L sodium phosphate, and 4 mmol/L ammonium molybdate. The mixture was incubated at 90°C for 90 mins using a water bath. After cooling down to room temperature, the samples were centrifuged at 5000 rpm for 5 mins to obtain the supernatants. The absorbance of the supernatants was measured at 695 nm using a spectrophotometer. Ascorbic acid at a 0–125 μg/mL concentration was used to prepare a standard curve. The activity of extracts was expressed as ascorbic acid equivalent (AAE) mg per g of the extracts.

2.2.6. Kirby Bauer Assay

The antibacterial activity of the extracts was tested using Kirby Bauer diffusion method [31]. Briefly, the extract at a concentration of 100 mg/mL was prepared in DMSO. Different bacteria including Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (E. coli, Pseudomonas aeruginosa) were cultured in Mueller–Hinton broth for 3 h at 37°C, 200 rpm shaking. The cell density was determined by measuring optical density at 600 nm using spectrophotometer. The bacterial suspensions were diluted to 108 CFU/mL, then spread on the surface of Mueller-Hinton agar plates using sterile swabs. 6 mm Ø wells were created and 20 µL the extracts were then supplemented into each well. The plates were incubated at 4°C for 2 h to allow the diffusion of the active compounds in the medium. The plates were incubated at 37°C for 16–18 h. Plates treated with 20 µL DMSO were used as negative controls and ciprofloxacin (5 µg/well) was used as positive controls. The antibacterial activity was determined by measuring the diameter of the inhibition zone to the nearest millimeter.

2.2.7. Determination of Minimum Inhibitory Concentration

The minimum inhibitory concentration (MIC) of the extracts was carried out by using the broth microdilution method [31]. The test was performed using sterile polystyrene 96-well plates. 100 µL of autoclaved Mueller-Hinton broth supplemented with different concentrations of the extracts was added to each well. Subsequently, 5 µL of the bacteria including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli at the concentration of approximately 108 CFU/mL was added. The plate was shaken and incubated at 37°C for 24 h. 0.1% DMSO was used as a negative control and ciprofloxacin was used as a positive control. MIC was defined as the lowest concentration of the extract at which the microorganisms showed no visible growth.

2.2.8. Statistical Analysis

All the data are presented as the mean ± standard error (SE). Student's t-test was used to determine the statistical significance of group means. All tests were two-sided and p values less than 0.05 were considered statistically significant.

3. Results

3.1. Determination of Total Phenolic Content

TPC of different extracts was estimated using Folin–Ciocalteu method. Gallic acid was used as a standard. The results were expressed as microgram of GAE per milligram of the extracts (Table 2). In consequence, many Garcinia extracts showed high TPC. Notably, the ME of G. hopii and G. tinctoria and EAE of G. planchonii and G. tinctoria showed high TPC with the values of 60.1, 70.8, 61, and 74.1 µg GAE/mg, respectively. Among tested Garcinia species, G. tinctoria exhibited high TPC in both EAE and ME. Conversely, there was low TPC in all extracts of G. fusca, G. gaudichaudii, and G. nigrolineata.

Table 2

The total polyphenolic content of various species of genus Garcinia.

Garcinia species	Extracts	Total polyphenolic content (µg QE/mg extract)
G. fusca	HE	20.58 ± 2.3
	EAE	45.18 ± 4.4
	ME	35.17 ± 2.5
G. hopii	HE	22.14 ± 5.1
	EAE	20.22 ± 2.4
	ME	60.09 ± 4.3
G. planchonii	HE	39.53 ± 2.5
	EAE	60.99 ± 5.6
	ME	17.16 ± 2.1
G. nigrolineata	HE	17.89 ± 2.5
	EAE	34.78 ± 3.4
	ME	29.99 ± 2.4
G. gaudichaudii	HE	12.34 ± 1.5
	EAE	41.59 ± 4.3
	ME	15.76 ± 3.4
G. tinctoria	HE	27.79 ± 3.8
	EAE	74.12 ± 4.9
	ME	70.84 ± 3.6

3.2. DPPH Assay

The free radical scavenging of the extracts was estimated using DPPH assay. The results were expressed in percentage values of inhibition of radicals (Figure 1). Both EAE of G. tinctoria and ME of G. nigrolineata comparably scavenged the free radicals with the inhibition of approximately 83.5%–87% (IC50 = 1.5 µg/mL and 2.5 µg/mL, resp.). The EAE of G. planchonii and G. fusca and ME of G. hopii showed comparable scavenging property, approximately 71.45%, 68.6%, and 65%, respectively. No obvious inhibition was observed in other extracts. Ascorbic acid was used as a positive control, exhibiting the scavenging of 95% with the IC50 of 0.78 µg/mL.

Figure 1

The free radical scavenging capability of extracts of Garcinia species using DPPH assay. (a) The activities of various extracts. (b) The activities of different species. The extracts were supplemented with DPPH (dissolved in methanol), then shaken vigorously, and incubated in dark at room temperature for 30 mins. Subsequently, the absorbance was measured at 517 nm using a spectrophotometer. Ascorbic acid was used as a positive control and 0.1 mM DMSO was used as a negative control. The most bioactive extract of each species was chosen to compare with others.

3.3. Hydroxyl Radical Scavenging Assay

The scavenging of OH− was estimated following the procedure as described by Smirnoff and Cumbes [32]. The present study revealed the high OH− radical scavenging activity of ME of G. planchonii (98%) with the IC50 of 178 µg/mL, followed by ME of G. hopii (56%). Low inhibition was attained in other extracts. Ascorbic acid was used as a positive control which showed the maximum scavenging of 94% with the IC50 of 5.5 µg/mL (Figure 2).

Figure 2

The hydroxyl radical scavenging capability of extracts of different Garcinia species. The extracts were supplemented with FeSO4.7H2O, salicylic acid, and H2O2, followed by being mixed and incubated at 37°C for 30 mins before applied onto a spectrophotometer to measure the absorbance at 562 nm. Ascorbic acid was used as a positive control and 0.1 mM DMSO was used as a negative control.

3.4. Total Antioxidant Capacity Assay

The TAC of the extracts was evaluated using phosphomolybdate assay, measuring the ability of a sample to destroy a free radical by transferring an electron to the latter. Ascorbic acid was used to provide a standard curve and the TAC was expressed as milligram of AAE per gram of the extract. Table 3 indicates the high TAC of G. tinctoria. Particularly, the TAC of ME, EAE, and HE of G. tinctoria was 265.9, 169.8, and 135.6 mg AAE/g extract, respectively. Comparably, the TACs of ME of G. hopii and EAE of G. planchonii were 208.2 and 160.1 mg AAE/g extract, respectively.

Table 3

The total antioxidant capacity of the extracts of different Garcinia species.

Garcinia species	Extracts	Total antioxidant capacity (mg AAE/g extract)
G. fusca	HE	0
	EAE	130.16 ± 5.3
	ME	34.56 ± 2.1
G. hopii	HE	2.57 ± 0.5
	EAE	91.77 ± 2.4
	ME	209.22 ± 7.5
G. planchonii	HE	0
	EAE	160.16 ± 5.5
	ME	35.08 ± 6.3
G. nigrolineata	HE	5.43 ± 1.2
	EAE	111.13 ± 4.5
	ME	87.26 ± 8.2
G. gaudichaudii	HE	4.35 ± 1.2
	EAE	70.48 ± 5.4
	ME	76.29 ± 7.3
G. tinctoria	HE	135.65 ± 8.4
	EAE	169.84 ± 4.5
	ME	265.97 ± 9.4

3.5. The Antimicrobial Activity

The antimicrobial property of the extracts was evaluated using diffusion Kirby Bauer assay. The diameters of the inhibition zone were described in Table 4. Subsequently, the antimicrobial extracts were screened for MIC using the microdilution method. Table 5 showed that EAE of G. gaudichaudii was effective against Gram-positive bacteria (S. aureus and B. subtilis) with an MIC of approximately 15.625–25 µg/mL whereas ME of G. planchonii was effective against both Gram-positive bacteria (S. aureus and B. subtilis) (MIC = 160 µg/mL) and Gram-negative bacteria (E. coli, P. aeruginosa) (MIC = 75 µg/mL). However, the MICs of both extracts were higher compared to that of ciprofloxacin (MIC = 0.25 µg/mL and 0.016 µg/mL against Gram-positive and Gram-negative bacteria, subsequently).

Table 4

The inhibition zone diameter of extracts of different Garcinia species on bacterial strains.

Garcinia species	Extracts	Inhibition zone diameter (mm)
		S. aureus	B. subtilis	P. aeruginosa	E. coli
Ciprofloxacin		17	15	23	32
G. fusca	HE	8	—	8	—
	EAE	12.5 ± 2.5	11 ± 2.3	8.5 ± 1.5	—
	ME	—	—	—	—
G. hopii	HE	—	—	—	—
	EAE	9 ± 1.2	—	9 ± 1.3	—
	ME	10.5 ± 2.3	—	7 ± 1	—
G. planchonii	HE	8 ± 1.4	—	8 ± 1.5	—
	EAE	12 ± 1.7	—	—	—
	ME	17 ± 2.5	16 ± 1.2	15 ± 1.5	20 ± 1.2
G. nigrolineata	HE	—	—	—	—
	EAE	9 ± 1.3	—	9 ± 1.8	8 ± 1.4
	ME	—	—	9 ± 1.4	—
G. gaudichaudii	HE	—	—	—	—
	EAE	17 ± 1	14 ± 0.7	7.5 ± 1.5	—
	ME	8 ± 1.7	—	8 ± 2.3	—
G. tinctoria	HE	10 ± 2.1	—	—	—
	EAE	12 ± 1.9	—	—	—
	ME	10 ± 0.9	—	—	—

Table 5

The MICs of extracts of different Garcinia species on bacterial strains.

The extracts	MICs on bacterial strains (µg/mL)
	S. aureus	B. subtilis	E. coli	P. aeruginosa
Ciprofloxacin	0.25	0.25	0.016	0.016
EAE of G. fusca	120	50	100	100
ME of G. hopii	140	200	200	100
ME of G. planchonii	160	160	75	75
EAE of G. gaudichaudii	15.625	25	100	100

4. Discussion

In this study, we collected six species of Garcinia including G. fusca, G. planchonii, G. hopii, G. tinctoria, G. nigrolineata, and G. gaudichaudii and then extracted them using different solvents, named hexane, ethyl acetate, and methanol. The phytochemicals of the extract relating to polyphenol were measured and the bioactivity including antioxidant and antimicrobial capacity was determined. The results indicated that ME and EAE of G. tinctoria showed promising antioxidant property while EAE of G. gaudichaudii and ME of G. planchonii possessed high antimicrobial activity.

Phenolic compounds are secondary metabolites containing at least one phenol unit in the structure. They are phytochemicals found in many plants which have a function as substances protecting plants from oxidative stress by neutralizing the free radicals by donating hydrogen, reducing and chelating metal, and capturing singlet oxygen [33]. Additionally, these metabolites break the free radical chain reaction by forming a relatively stable phenoxy-radical intermediate [34]. They are antioxidants which are used to treat various diseases relating to oxidative stress including cancer, cardiovascular diseases, Alzheimer's disease, and aging-related diseases [35, 36]. Therefore, isolating the extracts containing high phenolic compounds is required. In the present study, of the six, G. tinctoria showed the highest TPC (74.1 µg GAE/mg) (Table 2). Compared to HE, ME and EAE possessed higher TPC. The difference in solvent polarity determines the components in the extracts. The high polarity (methanol) and medium polarity (ethyl acetate) solvents enabled the isolation of more effective polyphenol compounds. Previous studies revealed the capacity of ethyl acetate and methanol in the extraction of high bioactive compounds [37-39]. Therefore, the choice of a suitable solvent is important in the isolation of natural extracts or compounds.

The DPPH assay is a frequently used technique to determine the radical scavenging capacity of plant-based extracts because of its quick and responsive features, which involve simple conventional laboratory equipment. The results showed that ME and EAE exhibited higher free radical scavenging ability compared to HE. Among them, EAE of G. tinctoria possessed the strongest free radical scavenging capacity with the inhibition of approximately 83.5% at IC50 of 1.5 µg/mL (Figure 2). The TPC results were in accordance with the antioxidant activity determined by DPPH and TAC assays (Table 2, Figure 1) which demonstrated that the antioxidant activity of G. tinctoria was mainly due to hampering free radicals of phenolic compounds in the extract. Many studies mentioned effective free radical inhibition of the phenolic-rich extraction [40-42]. However, the polyphenol content was not in accordance with the hydroxyl scavenging capacity (Table 2 and Figure 2). In particular, ME of G. planchonii showed the strongest hydroxyl scavenging activity with low polyphenol content (approximately 17.16 µg GAE/mg). Therefore, other compounds might contribute to this activity of the extract.

In terms of antimicrobial activity, ME of G. planchonii arrested the growth of both Gram-positive and Gram-negative bacterial strains while EAE of G. gaudichaudii hampered the proliferation of Gram-positive bacteria (Tables 4 and 5). However, the activities of both extracts were lower compared to those of ciprofloxacin (positive control). The poorer activity may be due to the interference with the active compounds of other components in the extract. Mostly the extracts or compounds target Gram-positive strains better than Gram-negative strains. The difference might be explained by the distinction in the structure of peptidoglycan, presence of outer membrane, receptors or lipids, cross-linking, and the activity of autolytic enzymes that determine the penetration, binding, and action of the compounds [43]. A study by Sunitha Janardhanan et al. demonstrated that crude chloroform extract of G. mangostana pericarp effectively inhibited Gram-positive bacteria including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus oralis, and Lactobacillus acidophilus [44]. Besides, other natural extracts or compounds also exhibited a higher sensitivity to Gram-positive bacteria [45-47].

5. Conclusions

This study was conducted by extracting different Garcinia species grown in Vietnam using various solvents. The total polyphenol content and bioactivity including antioxidant and antimicrobial activities were evaluated. Our results demonstrated that the EAE of G. tinctoria showed promising antioxidant capacity. In the antimicrobial assays, ME of G. planchonii was efficient against both Gram-positive bacteria (S. aureus and B. subtilis) (MIC = 160 µg/mL) and Gram-negative bacteria (E. coli, P. aeruginosa) (MIC = 75 µg/mL) while EAE of G. gaudichaudii was effective against Gram-positive bacteria (S. aureus and B. subtilis) with the MIC of approximately 15.625–25 µg/mL. Taken together, our results elucidated the antioxidation and antimicrobial property of several Garcinia extracts.