Deesha Bhaumik1, Divya Manikandan2, Betsy Foxman3. 1. Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI, United States. Electronic address: deesha@umich.edu. 2. University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, United States. Electronic address: divmani@umich.edu. 3. Center of Molecular and Clinical Epidemiology of Infectious Diseases, Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI, United States. Electronic address: bfoxman@umich.edu.
Abstract
OBJECTIVE: To review published oral microbiome studies and create a comprehensive list of bacterial species found in saliva and dental plaque among healthy children and adults associated with presence of carious lesions and caries-free state (oral health). DESIGN: This review followed PRISMA-ScR guidelines. We searched published studies querying PUBMED and EMBASE using the following keywords: (plaque OR saliva) AND caries AND (next generation sequencing OR checkerboard OR 16s rRNA or qPCR). Studies were limited to human studies published in English between January 1, 2010 and June 24, 2020 that included > 10 caries-active and > 10 caries-free participants, and assessed the entire bacterial community. RESULTS: Our search strategy identified 298 articles. After exclusion criteria, 22 articles remained; we considered 2 studies that examined saliva and plaque as separate studies, for a total of 24 studies. Species associated with caries or oral health varied widely among studies reviewed, with notable differences by age and biologic sample type. No bacterial species was associated with caries in all studies. Streptococcus mutans was found more frequently among those with caries (14/24 (58.3 %)) and Fusobacterium periodonticum was found more frequently among those that were caries-free (5/24 (20.8 %)). CONCLUSION: No bacterial species was associated with caries or oral health across all studies supporting multiple pathways to cariogenesis. However, the variation may be due to sampling at different time points during caries development, varying methods of specimen sampling, storage, sequencing or analysis or differences in host factors such as age.
OBJECTIVE: To review published oral microbiome studies and create a comprehensive list of bacterial species found in saliva and dental plaque among healthy children and adults associated with presence of carious lesions and caries-free state (oral health). DESIGN: This review followed PRISMA-ScR guidelines. We searched published studies querying PUBMED and EMBASE using the following keywords: (plaque OR saliva) AND caries AND (next generation sequencing OR checkerboard OR 16s rRNA or qPCR). Studies were limited to human studies published in English between January 1, 2010 and June 24, 2020 that included > 10 caries-active and > 10 caries-free participants, and assessed the entire bacterial community. RESULTS: Our search strategy identified 298 articles. After exclusion criteria, 22 articles remained; we considered 2 studies that examined saliva and plaque as separate studies, for a total of 24 studies. Species associated with caries or oral health varied widely among studies reviewed, with notable differences by age and biologic sample type. No bacterial species was associated with caries in all studies. Streptococcus mutans was found more frequently among those with caries (14/24 (58.3 %)) and Fusobacterium periodonticum was found more frequently among those that were caries-free (5/24 (20.8 %)). CONCLUSION: No bacterial species was associated with caries or oral health across all studies supporting multiple pathways to cariogenesis. However, the variation may be due to sampling at different time points during caries development, varying methods of specimen sampling, storage, sequencing or analysis or differences in host factors such as age.
Authors: J Xiao; A Grier; R C Faustoferri; S Alzoubi; A L Gill; C Feng; Y Liu; R G Quivey; D T Kopycka-Kedzierawski; H Koo; S R Gill Journal: J Dent Res Date: 2018-07-26 Impact factor: 6.116
Authors: Isabela N Rôças; Flávio R F Alves; Caio T C C Rachid; Kenio C Lima; Isauremi V Assunção; Patrícia N Gomes; José F Siqueira Journal: PLoS One Date: 2016-05-02 Impact factor: 3.240