Luiz Gustavo Dal Oglio da Rocha1,2, Victoria Stadler Tasca Ribeiro3,2, Ana Paula de Andrade3,2, Geiziane Aparecida Gonçalves3, Letícia Kraft3, Juliette Cieslinski3, Paula Hansen Suss3,2, Felipe Francisco Tuon4,5. 1. Hospital Universitário Cajuru - HUC, Spine Department. Pontifícia, Universidade Católica do Paraná (PUCPR), Avenida São José, 300, Curitiba, Paraná, 80050-350, Brazil. 2. School of Medicine, Health Sciences Department, Pontifícia Universidade Católica do Paraná (PUCPR), Rua Imaculada Conceição, 1155, Curitiba, Paraná, 80215-901, Brazil. 3. Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná (PUCPR), Rua Imaculada Conceição, 1155, Curitiba, Paraná, 80215-901, Brazil. 4. Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná (PUCPR), Rua Imaculada Conceição, 1155, Curitiba, Paraná, 80215-901, Brazil. felipe.tuon@pucpr.br. 5. School of Medicine, Health Sciences Department, Pontifícia Universidade Católica do Paraná (PUCPR), Rua Imaculada Conceição, 1155, Curitiba, Paraná, 80215-901, Brazil. felipe.tuon@pucpr.br.
Abstract
BACKGROUND: Titanium and polyether-ether-ketone (PEEK) interbody cages are commonly used for spine fusion. Few data are known about bacterial and yeast biofilms formation in these implants. The aim of this study was to compare Staphylococcus aureus and Candida albicans biofilm formation in the surface of two different interbody devices used routinely in spine surgery. METHODS: Six bodies of proof specimens of PEEK and titanium alloy were used for microbiological tests, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Experimental biofilm was produced with Staphylococcus aureus and Candida albicans, followed by quantitative analysis of planktonic cells and sessile cells. The comparison between the medians of biofilm quantification between the two models was performed using the Mann-Whitney test and considered the statistical difference for a p < 0.05. RESULTS: In the S. aureus model, in both planktonic and sessile cell counts, titanium-alloy samples showed lower values for colony forming units per milliliter (UFC/mL) (p < 0.05). The evaluation through the optic density of planktonic and sessile cells showed lower values in the titanium-alloy samples, however, only statistically significant in planktonic cell count (p < 0.05). The count of planktonic yeast cells in PEEK was similar to titanium-alloy samples, while the count of sessile yeast cells in titanium alloy was lower when compared to PEEK (p < 0.05). CONCLUSION: Titanium-alloy models were associated with less staphylococcal and Candida biofilm formation when compared with PEEK.
BACKGROUND: Titanium and polyether-ether-ketone (PEEK) interbody cages are commonly used for spine fusion. Few data are known about bacterial and yeast biofilms formation in these implants. The aim of this study was to compare Staphylococcus aureus and Candida albicans biofilm formation in the surface of two different interbody devices used routinely in spine surgery. METHODS: Six bodies of proof specimens of PEEK and titanium alloy were used for microbiological tests, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Experimental biofilm was produced with Staphylococcus aureus and Candida albicans, followed by quantitative analysis of planktonic cells and sessile cells. The comparison between the medians of biofilm quantification between the two models was performed using the Mann-Whitney test and considered the statistical difference for a p < 0.05. RESULTS: In the S. aureus model, in both planktonic and sessile cell counts, titanium-alloy samples showed lower values for colony forming units per milliliter (UFC/mL) (p < 0.05). The evaluation through the optic density of planktonic and sessile cells showed lower values in the titanium-alloy samples, however, only statistically significant in planktonic cell count (p < 0.05). The count of planktonic yeast cells in PEEK was similar to titanium-alloy samples, while the count of sessile yeast cells in titanium alloy was lower when compared to PEEK (p < 0.05). CONCLUSION: Titanium-alloy models were associated with less staphylococcal and Candida biofilm formation when compared with PEEK.
Authors: Andrej Trampuz; Kerryl E Piper; Melissa J Jacobson; Arlen D Hanssen; Krishnan K Unni; Douglas R Osmon; Jayawant N Mandrekar; Franklin R Cockerill; James M Steckelberg; James F Greenleaf; Robin Patel Journal: N Engl J Med Date: 2007-08-16 Impact factor: 91.245
Authors: Teresa L Street; Nicholas D Sanderson; Bridget L Atkins; Andrew J Brent; Kevin Cole; Dona Foster; Martin A McNally; Sarah Oakley; Leon Peto; Adrian Taylor; Tim E A Peto; Derrick W Crook; David W Eyre Journal: J Clin Microbiol Date: 2017-05-10 Impact factor: 5.948
Authors: Luiz Gustavo Dal Oglio da Rocha; Victoria Stadler Tasca Ribeiro; Ana Paula de Andrade; Geiziane Aparecida Gonçalves; Letícia Kraft; Juliette Cieslinski; Paula Hansen Suss; Felipe Francisco Tuon Journal: Eur J Orthop Surg Traumatol Date: 2021-07-08