| Literature DB >> 34206634 |
Veronica Zampieri1, Cécile Hilpert2, Mélanie Garnier2, Yannick Gestin2, Sébastien Delolme2, Juliette Martin2, Pierre Falson2, Guillaume Launay2, Vincent Chaptal2.
Abstract
Detergents wrap around membrane proteins to form a belt covering the hydrophobic part of the protein serving for membrane insertion and interaction with lipids. The number of detergent monomers forming this belt is usually unknown to investigators, unless dedicated detergent quantification is undertaken, which for many projects is difficult to setup. Yet, having an approximate knowledge of the amount of detergent forming the belt is extremely useful, to better grasp the protein of interest in interaction with its direct environment rather than picturing the membrane protein "naked". We created the Det.Belt server to dress up membrane proteins and represent in 3D the bulk made by detergent molecules wrapping in a belt. Many detergents are included in a database, allowing investigators to screen in silico the effect of different detergents around their membrane protein. The input number of detergents is changeable with fast recomputation of the belt for interactive usage. Metrics representing the belt are readily available together with scripts to render quality 3D images for publication. The Det.Belt server is a tool for biochemists to better grasp their sample.Entities:
Keywords: amphipathic solvent; detergent belt; detergent quantification and modeling
Year: 2021 PMID： 34206634 DOI： 10.3390/membranes11070459
Source DB: PubMed Journal: Membranes (Basel) ISSN： 2077-0375