Gang Hu1, Shusen Zhai2, Sheng Yu3, Zhen Huang1, Ran Gao1. 1. Department of General Surgery, Strategic Support Force Characteristic Medical Center, Beijing, 100101, People's Republic of China. 2. Department of Oncology, Strategic Support Force Characteristic Medical Center, Beijing, 100101, People's Republic of China. 3. Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou City, Guangdong Province, 510515, People's Republic of China. ShengYuHepatobilio@163.com.
Abstract
BACKGROUND: Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. METHODS: In this study, circRHOBTB3, mature miR-18a, and miR-18a precursor in HCC and paired non-cancer tissues were detected by RT-qPCR. The role of circRHOBTB3 in the production of mature miR-18a was explored by transfecting circRHOBTB3 expression vector into HCC cells, followed by RT-qPCR to determine the expression of mature miR-18a and miR-18a precursor. The role of circRHOBTB3 and miR-18a in HCC cell proliferation was studied using CCK-8 assay. RESULTS: CircRHOBTB3 was under-expressed in HCC compared to normal tissues. In HCC cells, circRHOBTB3 overexpression decreased mature miR-18a level but not miR-18a precursor. Cell proliferation analysis showed that circRHOBTB3 overexpression decreased cell proliferation while miR-18a overexpression increased cell proliferation. Moreover, circRHOBTB3 suppressed the role of miR-18a in cell proliferation. CONCLUSIONS: CircRHOBTB3 is downregulated in HCC and may suppress cell proliferation by reducing miR-18a production.
BACKGROUND: Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. METHODS: In this study, circRHOBTB3, mature miR-18a, and miR-18a precursor in HCC and paired non-cancer tissues were detected by RT-qPCR. The role of circRHOBTB3 in the production of mature miR-18a was explored by transfecting circRHOBTB3 expression vector into HCC cells, followed by RT-qPCR to determine the expression of mature miR-18a and miR-18a precursor. The role of circRHOBTB3 and miR-18a in HCC cell proliferation was studied using CCK-8 assay. RESULTS:CircRHOBTB3 was under-expressed in HCC compared to normal tissues. In HCC cells, circRHOBTB3 overexpression decreased mature miR-18a level but not miR-18a precursor. Cell proliferation analysis showed that circRHOBTB3 overexpression decreased cell proliferation while miR-18a overexpression increased cell proliferation. Moreover, circRHOBTB3 suppressed the role of miR-18a in cell proliferation. CONCLUSIONS:CircRHOBTB3 is downregulated in HCC and may suppress cell proliferation by reducing miR-18a production.
Authors: Rosa Zampino; Maria A Pisaturo; Grazia Cirillo; Aldo Marrone; Margherita Macera; Luca Rinaldi; Maria Stanzione; Emanuele Durante-Mangoni; Ivan Gentile; Evangelista Sagnelli; Giuseppe Signoriello; Emanuele Miraglia Del Giudice; Luigi E Adinolfi; Nicola Coppola Journal: Ann Hepatol Date: 2015 Jan-Feb Impact factor: 2.400