Heather Wilson-Robles1, Tasha Miller2, Jill Jarvis2, Jason Terrell3, Theresa Kathleen Kelly2,3, Thomas Bygott4, Mhammed Bougoussa5. 1. College of Veterinary Medicine, Small Animal Clinical Sciences Department, Texas A&M University, College Station, TX, 77843, USA. hwilson@cvm.tamu.edu. 2. College of Veterinary Medicine, Small Animal Clinical Sciences Department, Texas A&M University, College Station, TX, 77843, USA. 3. Volition America & Volition Veterinary Diagnostic Development, 13215 Bee Cave Parkway, Galleria Oaks B, Suite 125, Austin, Texas, 78738, USA. 4. Volition Diagnostics UK Ltd, 93-95 Gloucester Place, London, W1U 6JQ, UK. 5. Belgian Volition SRL, 22 Rue Phocas Lejeune, Parc Scientifique Crealys, 5032, Isnes, Belgium.
Abstract
BACKGROUND: Nucleosomes consist of DNA wrapped around a histone octamer core like thread on a spool to condense DNA as chromatin into chromosomes. Diseases such as cancer or inflammation lead to cell death, chromatin fragmentation and release of nucleosomes into the blood. The Nu.Q™ platform measures circulating nucleosomes in the blood of humans that result from disease and has been used to detect and identify cancer even at early stages. The objectives of this study are to quantify and better characterize nucleosomes in dogs with various stages of hemangiosarcoma (HSA) using this ELISA-based assay. Samples from 77 dogs with a confirmed diagnosis of hemangiosarcoma and 134 healthy controls were utilized for this study. The HSA samples were recruited from the Texas A&M University Small Animal Clinic (TAMU-SAC) or purchased from biobanks. All control samples were recruited from the TAMU-SAC. RESULTS: Dogs with hemangiosarcoma had a 6.6-fold increase in their median plasma nucleosome concentrations compared to controls (AUC 92.9 %). Elevated nucleosome concentrations were seen at all stages of disease and nucleosome concentrations increased with the stage of the disease. CONCLUSIONS: Plasma nucleosome concentrations are a reliable way to differentiate dogs with hemangiosarcoma from healthy dogs. Further testing is underway to better characterize cancer associated HSA circulating nucleosomes and optimize future diagnostics for canine HSA detection.
BACKGROUND: Nucleosomes consist of DNA wrapped around a histone octamer core like thread on a spool to condense DNA as chromatin into chromosomes. Diseases such as cancer or inflammation lead to cell death, chromatin fragmentation and release of nucleosomes into the blood. The Nu.Q™ platform measures circulating nucleosomes in the blood of humans that result from disease and has been used to detect and identify cancer even at early stages. The objectives of this study are to quantify and better characterize nucleosomes in dogs with various stages of hemangiosarcoma (HSA) using this ELISA-based assay. Samples from 77 dogs with a confirmed diagnosis of hemangiosarcoma and 134 healthy controls were utilized for this study. The HSA samples were recruited from the Texas A&M University Small Animal Clinic (TAMU-SAC) or purchased from biobanks. All control samples were recruited from the TAMU-SAC. RESULTS:Dogs with hemangiosarcoma had a 6.6-fold increase in their median plasma nucleosome concentrations compared to controls (AUC 92.9 %). Elevated nucleosome concentrations were seen at all stages of disease and nucleosome concentrations increased with the stage of the disease. CONCLUSIONS: Plasma nucleosome concentrations are a reliable way to differentiate dogs with hemangiosarcoma from healthy dogs. Further testing is underway to better characterize cancer associated HSA circulating nucleosomes and optimize future diagnostics for canine HSA detection.
Authors: Frank Diehl; Kerstin Schmidt; Michael A Choti; Katharine Romans; Steven Goodman; Meng Li; Katherine Thornton; Nishant Agrawal; Lori Sokoll; Steve A Szabo; Kenneth W Kinzler; Bert Vogelstein; Luis A Diaz Journal: Nat Med Date: 2007-07-31 Impact factor: 53.440
Authors: Stefan Holdenrieder; Dorothea Nagel; Andreas Schalhorn; Volker Heinemann; Ralf Wilkowski; Joachim von Pawel; Hannelore Raith; Knut Feldmann; Andreas E Kremer; Susanne Müller; Sandra Geiger; Gerhard F Hamann; Dietrich Seidel; Petra Stieber Journal: Ann N Y Acad Sci Date: 2008-08 Impact factor: 5.691
Authors: H M Wilson-Robles; T Bygott; T K Kelly; T M Miller; P Miller; M Matsushita; J Terrell; M Bougoussa; T Butera Journal: BMC Vet Res Date: 2022-08-31 Impact factor: 2.792