Sevim Karakaş Çelik1, Güneş Çakmak Genç1, Nihal Pişkin2, Bilgehan Açikgöz3, Bülent Altinsoy4, Başak Kurucu Işsiz1, Ahmet Dursun1. 1. Department of Medical Genetics, Zonguldak Bulent Ecevit University, Zonguldak, Turkey. 2. Department of Infectious Diseases and Clinical Microbiology, Zonguldak Bulent Ecevit University, Zonguldak, Turkey. 3. Department of Public Health, Zonguldak Bulent Ecevit University, Zonguldak, Turkey. 4. Department of Pulmonary Medicine, Zonguldak Bulent Ecevit University, Zonguldak, Turkey.
Abstract
Coronavirus disease 2019 (COVID-19) is an infectious disease, that's the reason for the currently ongoing pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme (ACE2) has been recognized as the specific receptor of the SARS-CoV-2 virus. Although the possible effect of ACE2 gene polymorphism remains unknown, human ACE2 receptor expression influences SARS-CoV-2 susceptibility and COVID-19 disease outcome. In this study, we aimed to investigate the relationship between ACE gene I/D polymorphism, ACE2 receptor gene polymorphism, and COVID-19 severity. METHODS: ACE gene insertion/deletion (I/D) polymorphism and ACE2 receptor gene rs2106809 and rs2285666 polymorphisms were determined using polymerase chain reaction (PCR) and PCR-based restriction fragment length polymorphism (RFLP) methods, respectively in 155 COVID-19 patients who were divided into 3 groups (mild, moderate, and severe) according to clinical symptoms. RESULTS: However, the distribution of genotype and allele frequencies of ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not statistically significant in all groups. CONCLUSIONS: In conclusion, in the study population, ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not associated with the severity of COVID-19 infection. Although, ACE2 receptor gene expression may affect the susceptibility to COVID-19, there is no existing evidence that the ACE or ACE2 gene polymorphisms are directly associated with COVID-19 severity. Interindividual differences in COVID-19 severity might be related to epigenetic mechanisms of ACE2 receptor gene expression or variations in other genes suggested to have critical role in COVID-19 pathogenesis such as proinflammatory cytokines, coagulation indicators. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
Coronavirus disease 2019 (COVID-19) is an infectious disease, that's the reason for the currently ongoing pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme (ACE2) has been recognized as the specific receptor of the SARS-CoV-2 virus. Although the possible effect of ACE2 gene polymorphism remains unknown, humanACE2 receptor expression influences SARS-CoV-2 susceptibility and COVID-19 disease outcome. In this study, we aimed to investigate the relationship between ACE gene I/D polymorphism, ACE2 receptor gene polymorphism, and COVID-19 severity. METHODS:ACE gene insertion/deletion (I/D) polymorphism and ACE2 receptor gene rs2106809 and rs2285666 polymorphisms were determined using polymerase chain reaction (PCR) and PCR-based restriction fragment length polymorphism (RFLP) methods, respectively in 155 COVID-19patients who were divided into 3 groups (mild, moderate, and severe) according to clinical symptoms. RESULTS: However, the distribution of genotype and allele frequencies of ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not statistically significant in all groups. CONCLUSIONS: In conclusion, in the study population, ACE gene I/D, ACE2 receptor gene rs2106809, and rs2285666 polymorphisms were not associated with the severity of COVID-19infection. Although, ACE2 receptor gene expression may affect the susceptibility to COVID-19, there is no existing evidence that the ACE or ACE2 gene polymorphisms are directly associated with COVID-19 severity. Interindividual differences in COVID-19 severity might be related to epigenetic mechanisms of ACE2 receptor gene expression or variations in other genes suggested to have critical role in COVID-19 pathogenesis such as proinflammatory cytokines, coagulation indicators. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
Authors: Mariana Angulo-Aguado; David Corredor-Orlandelli; Juan Camilo Carrillo-Martínez; Mónica Gonzalez-Cornejo; Eliana Pineda-Mateus; Carolina Rojas; Paula Triana-Fonseca; Nora Constanza Contreras Bravo; Adrien Morel; Katherine Parra Abaunza; Carlos M Restrepo; Dora Janeth Fonseca-Mendoza; Oscar Ortega-Recalde Journal: Front Med (Lausanne) Date: 2022-06-20
Authors: João Locke Ferreira de Araújo; Diego Menezes; Renato Santana de Aguiar; Renan Pedra de Souza Journal: Front Genet Date: 2022-04-01 Impact factor: 4.599
Authors: Maria Sabater Molina; Elisa Nicolás Rocamora; Asunción Iborra Bendicho; Elisa García Vázquez; Esther Zorio; Fernando Domínguez Rodriguez; Cristina Gil Ortuño; Ana Isabel Rodríguez; Antonio J Sánchez-López; Rubén Jara Rubio; Antonio Moreno-Docón; Pedro J Marcos; Pablo García Pavía; Roberto Barriales Villa; Juan R Gimeno Blanes Journal: PLoS One Date: 2022-02-04 Impact factor: 3.240