Lizhou Wang1, Tianpeng Jiang1, Xueqing Huang1, Shi Zhou1. 1. Department of Interventional Radiology, The Affiliated Hospital of Guizhou Medical University Guiyang, Guizhou Province, China.
Abstract
OBJECTIVE: The study aimed to explore the role of miR-25 and the notch signaling pathway in the memory capacity and brain tissue of mice with central nervous system (CNS) infections. METHODS: A bioinformatics website and the dual-luciferase reporter assay were used to analyze the targeting relationship between miR-25 and Notch1. The mice were randomized into 7 groups (n=10 per group), including the normal group, the model group (lipopolysaccharide at a dose of 500 μg/kg for the model establishment), the NC group, the miR-25 mimic group, the miR-25 inhibitor group, the DAPT group, and the miR-25 inhibitor + DAPT group. qRT-PCR and western blot were used to measure the miR-25, Notch1, and Hes5 expression levels in the hippocampal CA1 region of the mice's brains, along with the cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) levels in the mice's hippocampi. RESULTS: Compared with the normal mice, the model mice had up-regulated miR-25, COX-2, and iNOS expressions and down-regulated Notch1 and Hes5 expressions, lower superoxide dismutase (SOD) levels in the hippocampi, and higher malondialdehyde (MDA) levels. Compared with the model group, the miR-25 mimic and DAPT groups had down-regulated Notch1 and Hes5 expressions, lower learning and memory capacities and SOD levels, higher MDA levels, and up-regulated COX-2 and iNOS expressions. CONCLUSION: Down-regulating miR-25 may improve the memory capacity in mice with CNS infections by activating the Notch signaling pathway. AJTR
OBJECTIVE: The study aimed to explore the role of miR-25 and the notch signaling pathway in the memory capacity and brain tissue of mice with central nervous system (CNS) infections. METHODS: A bioinformatics website and the dual-luciferase reporter assay were used to analyze the targeting relationship between miR-25 and Notch1. The mice were randomized into 7 groups (n=10 per group), including the normal group, the model group (lipopolysaccharide at a dose of 500 μg/kg for the model establishment), the NC group, the miR-25 mimic group, the miR-25 inhibitor group, the DAPT group, and the miR-25 inhibitor + DAPT group. qRT-PCR and western blot were used to measure the miR-25, Notch1, and Hes5 expression levels in the hippocampal CA1 region of the mice's brains, along with the cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) levels in the mice's hippocampi. RESULTS: Compared with the normal mice, the model mice had up-regulated miR-25, COX-2, and iNOS expressions and down-regulated Notch1 and Hes5 expressions, lower superoxide dismutase (SOD) levels in the hippocampi, and higher malondialdehyde (MDA) levels. Compared with the model group, the miR-25 mimic and DAPT groups had down-regulated Notch1 and Hes5 expressions, lower learning and memory capacities and SOD levels, higher MDA levels, and up-regulated COX-2 and iNOS expressions. CONCLUSION: Down-regulating miR-25 may improve the memory capacity in mice with CNS infections by activating the Notch signaling pathway. AJTR
Authors: Nataliya Razumilava; Steve F Bronk; Rory L Smoot; Christian D Fingas; Nathan W Werneburg; Lewis R Roberts; Justin L Mott Journal: Hepatology Date: 2011-12-19 Impact factor: 17.425
Authors: Christine Wahlquist; Dongtak Jeong; Agustin Rojas-Muñoz; Changwon Kho; Ahyoung Lee; Shinichi Mitsuyama; Alain van Mil; Woo Jin Park; Joost P G Sluijter; Pieter A F Doevendans; Roger J Hajjar; Mark Mercola Journal: Nature Date: 2014-03-12 Impact factor: 49.962