Literature DB >> 34140522

A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells.

Simon G P Funnell1,2,3, Babak Afrough1, John James Baczenas4, Neil Berry5, Kevin R Bewley1, Rebecca Bradford6, Clint Florence7, Yann Le Duff5, Mark Lewis7, Ryan V Moriarty4, Shelby L O Connor4, Karen L Osman1, Steven Pullan1, Sujatha Rashid6, Kevin S Richards1,8, Kimberly J Stemple7, Ivana Knezevic9.   

Abstract

An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.

Entities:  

Year:  2021        PMID： 34140522      PMCID： PMC8211640          DOI： 10.1038/s41541-021-00346-z

Source DB:  PubMed          Journal:  NPJ Vaccines        ISSN： 2059-0105            Impact factor:   7.344


  11 in total

1.  Mutations present in a low-passage Zika virus isolate result in attenuated pathogenesis in mice.

Authors:  Nisha K Duggal; Erin M McDonald; James Weger-Lucarelli; Seth A Hawks; Jana M Ritter; Hannah Romo; Gregory D Ebel; Aaron C Brault
Journal:  Virology       Date:  2019-02-07       Impact factor: 3.616

2.  Growth-Adaptive Mutations in the Ebola Virus Makona Glycoprotein Alter Different Steps in the Virus Entry Pathway.

Authors:  John B Ruedas; Catherine E Arnold; Gustavo Palacios; John H Connor
Journal:  J Virol       Date:  2018-09-12       Impact factor: 5.103

3.  Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation.

Authors:  Mart M Lamers; Anna Z Mykytyn; Tim I Breugem; Yiquan Wang; Douglas C Wu; Samra Riesebosch; Petra B van den Doel; Debby Schipper; Theo Bestebroer; Nicholas C Wu; Bart L Haagmans
Journal:  Elife       Date:  2021-04-09       Impact factor: 8.140

4.  Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein.

Authors:  Andrew D Davidson; Maia Kavanagh Williamson; Sebastian Lewis; Deborah Shoemark; Miles W Carroll; Kate J Heesom; Maria Zambon; Joanna Ellis; Philip A Lewis; Julian A Hiscox; David A Matthews
Journal:  Genome Med       Date:  2020-07-28       Impact factor: 11.117

5.  Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.

Authors:  Jennifer Harcourt; Azaibi Tamin; Xiaoyan Lu; Shifaq Kamili; Senthil K Sakthivel; Janna Murray; Krista Queen; Ying Tao; Clinton R Paden; Jing Zhang; Yan Li; Anna Uehara; Haibin Wang; Cynthia Goldsmith; Hannah A Bullock; Lijuan Wang; Brett Whitaker; Brian Lynch; Rashi Gautam; Craig Schindewolf; Kumari G Lokugamage; Dionna Scharton; Jessica A Plante; Divya Mirchandani; Steven G Widen; Krishna Narayanan; Shinji Makino; Thomas G Ksiazek; Kenneth S Plante; Scott C Weaver; Stephen Lindstrom; Suxiang Tong; Vineet D Menachery; Natalie J Thornburg
Journal:  Emerg Infect Dis       Date:  2020-06-17       Impact factor: 6.883

6.  Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates.

Authors:  Kizzmekia S Corbett; Barbara Flynn; Kathryn E Foulds; Joseph R Francica; Seyhan Boyoglu-Barnum; Anne P Werner; Britta Flach; Sarah O'Connell; Kevin W Bock; Mahnaz Minai; Bianca M Nagata; Hanne Andersen; David R Martinez; Amy T Noe; Naomi Douek; Mitzi M Donaldson; Nadesh N Nji; Gabriela S Alvarado; Darin K Edwards; Dillon R Flebbe; Evan Lamb; Nicole A Doria-Rose; Bob C Lin; Mark K Louder; Sijy O'Dell; Stephen D Schmidt; Emily Phung; Lauren A Chang; Christina Yap; John-Paul M Todd; Laurent Pessaint; Alex Van Ry; Shanai Browne; Jack Greenhouse; Tammy Putman-Taylor; Amanda Strasbaugh; Tracey-Ann Campbell; Anthony Cook; Alan Dodson; Katelyn Steingrebe; Wei Shi; Yi Zhang; Olubukola M Abiona; Lingshu Wang; Amarendra Pegu; Eun Sung Yang; Kwanyee Leung; Tongqing Zhou; I-Ting Teng; Alicia Widge; Ingelise Gordon; Laura Novik; Rebecca A Gillespie; Rebecca J Loomis; Juan I Moliva; Guillaume Stewart-Jones; Sunny Himansu; Wing-Pui Kong; Martha C Nason; Kaitlyn M Morabito; Tracy J Ruckwardt; Julie E Ledgerwood; Martin R Gaudinski; Peter D Kwong; John R Mascola; Andrea Carfi; Mark G Lewis; Ralph S Baric; Adrian McDermott; Ian N Moore; Nancy J Sullivan; Mario Roederer; Robert A Seder; Barney S Graham
Journal:  N Engl J Med       Date:  2020-07-28       Impact factor: 91.245

7.  SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology.

Authors:  Natacha S Ogando; Tim J Dalebout; Jessika C Zevenhoven-Dobbe; Ronald W A L Limpens; Yvonne van der Meer; Leon Caly; Julian Druce; Jutte J C de Vries; Marjolein Kikkert; Montserrat Bárcena; Igor Sidorov; Eric J Snijder
Journal:  J Gen Virol       Date:  2020-09       Impact factor: 3.891

8.  LoFreq: a sequence-quality aware, ultra-sensitive variant caller for uncovering cell-population heterogeneity from high-throughput sequencing datasets.

Authors:  Andreas Wilm; Pauline Poh Kim Aw; Denis Bertrand; Grace Hui Ting Yeo; Swee Hoe Ong; Chang Hua Wong; Chiea Chuen Khor; Rosemary Petric; Martin Lloyd Hibberd; Niranjan Nagarajan
Journal:  Nucleic Acids Res       Date:  2012-10-12       Impact factor: 16.971

9.  Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples.

Authors:  Kuiama Lewandowski; Yifei Xu; Steven T Pullan; Sheila F Lumley; Dona Foster; Nicholas Sanderson; Alison Vaughan; Marcus Morgan; Nicole Bright; James Kavanagh; Richard Vipond; Miles Carroll; Anthony C Marriott; Karen E Gooch; Monique Andersson; Katie Jeffery; Timothy E A Peto; Derrick W Crook; A Sarah Walker; Philippa C Matthews
Journal:  J Clin Microbiol       Date:  2019-12-23       Impact factor: 5.948

10.  Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia.

Authors:  Leon Caly; Julian Druce; Jason Roberts; Katherine Bond; Thomas Tran; Renata Kostecki; Yano Yoga; William Naughton; George Taiaroa; Torsten Seemann; Mark B Schultz; Benjamin P Howden; Tony M Korman; Sharon R Lewin; Deborah A Williamson; Mike G Catton
Journal:  Med J Aust       Date:  2020-04-01       Impact factor: 12.776
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  10 in total

1.  Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment.

Authors:  Krist Helen Antunes; Ana Paula Duarte de Souza
Journal:  Methods Mol Biol       Date:  2022

2.  The Reassessed Potential of SARS-CoV-2 Attenuation for COVID-19 Vaccine Development-A Systematic Review.

Authors:  Marcin Goławski; Piotr Lewandowski; Iwona Jabłońska; Marcin Delijewski
Journal:  Viruses       Date:  2022-05-07       Impact factor: 5.818

3.  Propagation of SARS-CoV-2 in Calu-3 Cells to Eliminate Mutations in the Furin Cleavage Site of Spike.

Authors:  John James Baczenas; Hanne Andersen; Sujatha Rashid; David Yarmosh; Nikhita Puthuveetil; Michael Parker; Rebecca Bradford; Clint Florence; Kimberly J Stemple; Mark G Lewis; Shelby L O'Connor
Journal:  Viruses       Date:  2021-12-04       Impact factor: 5.818

Review 4.  Experimental Models of COVID-19.

Authors:  Luis A Caldera-Crespo; Michael J Paidas; Sabita Roy; Carl I Schulman; Norma Sue Kenyon; Sylvia Daunert; Arumugam R Jayakumar
Journal:  Front Cell Infect Microbiol       Date:  2022-01-05       Impact factor: 5.293

5.  The rapid progress in COVID vaccine development and implementation.

Authors:  Alan D T Barrett; Richard W Titball; Paul A MacAry; Richard E Rupp; Veronika von Messling; David H Walker; Nicolas V J Fanget
Journal:  NPJ Vaccines       Date:  2022-02-10       Impact factor: 7.344

6.  Identification, propagation and molecular characterization of SARS-CoV-2 delta variant isolated from Egyptian COVID-19 patients.

Authors:  Badriyah Alotaibi; Thanaa A El-Masry; Mohamed G Seadawy; Bassem E El-Harty; Asmaa Saleh; Ahmed F Gad; Mostafa F El-Hosseny; Yasmen F Mahran; Maisra M El-Bouseary
Journal:  Infect Genet Evol       Date:  2022-03-31       Impact factor: 4.393

7.  Mutation rate of SARS-CoV-2 and emergence of mutators during experimental evolution.

Authors:  Massimo Amicone; Vítor Borges; Maria João Alves; Joana Isidro; Líbia Zé-Zé; Sílvia Duarte; Luís Vieira; Raquel Guiomar; João Paulo Gomes; Isabel Gordo
Journal:  Evol Med Public Health       Date:  2022-03-29

8.  A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan.

Authors:  Po-Lin Lin; Ming-Sian Wu; Po-Chi Wu; Hsin-Mei Chen; Yi-Hsuan Peng; Jia-Chuan Hsu; Der-Yuan Wang
Journal:  Biologicals       Date:  2022-08-31       Impact factor: 1.760

9.  Isolation and Characterization of Five Severe Acute Respiratory Syndrome Coronavirus 2 Strains of Different Clades and Lineages Circulating in Eastern India.

Authors:  Bharati Singh; Kiran Avula; Sanchari Chatterjee; Ankita Datey; Arup Ghosh; Saikat De; Supriya Suman Keshry; Soumyajit Ghosh; Amol Ratnakar Suryawanshi; Rupesh Dash; Shantibhusan Senapati; Tushar K Beuria; Punit Prasad; Sunil Raghav; Rajeeb Swain; Ajay Parida; Gulam Hussain Syed; Soma Chattopadhyay
Journal:  Front Microbiol       Date:  2022-06-30       Impact factor: 6.064

Review 10.  Efficacy of Mouth Rinses and Nasal Spray in the Inactivation of SARS-CoV-2: A Systematic Review and Meta-Analysis of In Vitro and In Vivo Studies.

Authors:  Majdy Idrees; Bridget McGowan; Amr Fawzy; Abdulwahab Ali Abuderman; Ramesh Balasubramaniam; Omar Kujan
Journal:  Int J Environ Res Public Health       Date:  2022-09-25       Impact factor: 4.614
  10 in total

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