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Literature DB >> 3413482

Induction of an antibody that catalyzes the hydrolysis of an amide bond.

K D Janda¹, D Schloeder, S J Benkovic, R A Lerner.

Abstract

Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.

Entities: CellLine Chemical

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Year: 1988 PMID： 3413482 DOI： 10.1126/science.3413482

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

20 in total

1. Backbone resonance assignments for the Fv fragment of the catalytic antibody NPN43C9 with bound p-nitrophenol.

Authors: G J Kroon; M A Martinez-Yamout; J F Krebs; J Chung; H J Dyson; P E Wright
Journal: J Biomol NMR Date: 1999-09 Impact factor: 2.835

2. Changes in structure and dynamics of the Fv fragment of a catalytic antibody upon binding of inhibitor.

Authors: Gerard J A Kroon; Huaping Mo; Maria A Martinez-Yamout; H Jane Dyson; Peter E Wright
Journal: Protein Sci Date: 2003-07 Impact factor: 6.725

3. Construction and characterization of a single-chain catalytic antibody.

Authors: R A Gibbs; B A Posner; D R Filpula; S W Dodd; M A Finkelman; T K Lee; M Wroble; M Whitlow; S J Benkovic
Journal: Proc Natl Acad Sci U S A Date: 1991-05-01 Impact factor: 11.205

Review 4. Molecular recognition in antibodies and its application.

Authors: M C Tedford; W H Stimson
Journal: Experientia Date: 1991-12-01

5. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

Authors: A S Kang; C F Barbas; K D Janda; S J Benkovic; R A Lerner
Journal: Proc Natl Acad Sci U S A Date: 1991-05-15 Impact factor: 11.205

Review 6. Catalytic antibodies.

Authors: G M Blackburn; A S Kang; G A Kingsbury; D R Burton
Journal: Biochem J Date: 1989-09-01 Impact factor: 3.857

Induction of an antibody that catalyzes the hydrolysis of an amide bond.

1. Backbone resonance assignments for the Fv fragment of the catalytic antibody NPN43C9 with bound p-nitrophenol.

2. Changes in structure and dynamics of the Fv fragment of a catalytic antibody upon binding of inhibitor.

3. Construction and characterization of a single-chain catalytic antibody.

Review 4. Molecular recognition in antibodies and its application.

5. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

Review 6. Catalytic antibodies.

Review 7. Catalytic antibodies: a critical assessment.

Review 8. Immune recognition, antigen design, and catalytic antibody production.

Review 9. Hapten design for the generation of catalytic antibodies.

10. Induced-fit catalysis of corannulene bowl-to-bowl inversion.