Yan Liu1, Qiuying Li1, Tingsong Chen2, Tianhao Shen1, Xufeng Zhang3, Ping Song4, Lantao Liu1, Jianming Liu1, Tinghui Jiang5, Xiaofei Liang6. 1. Department of Interventional Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, Lanxi Road, Shanghai, 200333, China. 2. Second department of oncology, The Seventh People's Hospital Affiliated to Shanghai University of Chinese Medicine, Shanghai, China. 3. Department of Thoracic Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. 4. Huzhou Lieyuan Medical Laboratory Co., Ltd, No. 800, Rujiadian Road, Huzhou, 313009, China. 5. Department of Interventional Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, Lanxi Road, Shanghai, 200333, China. jiangtinghui@sina.com. 6. Huzhou Lieyuan Medical Laboratory Co., Ltd, No. 800, Rujiadian Road, Huzhou, 313009, China. xfliang86@126.com.
Abstract
BACKGROUND: Circulating tumor cells (CTCs) are the dominant factor leading to tumor metastasis. This study aims to investigate the effect of disparate sources of CTCs on the treatment and prognosis of patients with advanced tumors by analyzing the number and gene mutations change of CTCs in arterial and venous blood in patients with advanced tumors. RESULTS: A CTCs sorting system was constructed based on Vimentin-immunolipid magnetic balls (Vi-IMB) and EpCAM immunolipid magnetic balls (Ep-IMB). Results showed that the prepared Ep-IMB and Vi-IMB had lower cytotoxicity, better specificity and sensitivity. The number of arterial CTCs was higher than that of venous CTCs, with a statistically significant difference (P < 0.05). Moreover, the prognosis of the low positive group of total CTCs in arterial blood and venous blood was higher than that of the high positive group, with a statistical significance (P < 0.05). The genetic testing results showed that the targeted drug gene mutations in tissues, arterial CTCs and venous CTCs showed a complementary trend, indicating that there was heterogeneity among different tumor samples. CONCLUSIONS: CTCs in blood can be efficiently captured by the CTCs sorting system based on Vi-LMB/Ep-LMB, and CTCs detection in arterial blood can be utilized to more accurately evaluate the prognosis and predict postoperative progress. It is further confirmed that tumor samples from disparate sources are heterogeneous, providing a reference basis for gene mutation detection before clinical targeted drug treatment, and the detection of CTCs in arterial blood has more potential clinical application value. TRIAL REGISTRATION: The Ethics Committee of Putuo Hospital, PTEC-A-2019-18-1. Registered 24 September 2019.
BACKGROUND: Circulating tumor cells (CTCs) are the dominant factor leading to tumor metastasis. This study aims to investigate the effect of disparate sources of CTCs on the treatment and prognosis of patients with advanced tumors by analyzing the number and gene mutations change of CTCs in arterial and venous blood in patients with advanced tumors. RESULTS: A CTCs sorting system was constructed based on Vimentin-immunolipid magnetic balls (Vi-IMB) and EpCAM immunolipid magnetic balls (Ep-IMB). Results showed that the prepared Ep-IMB and Vi-IMB had lower cytotoxicity, better specificity and sensitivity. The number of arterial CTCs was higher than that of venous CTCs, with a statistically significant difference (P < 0.05). Moreover, the prognosis of the low positive group of total CTCs in arterial blood and venous blood was higher than that of the high positive group, with a statistical significance (P < 0.05). The genetic testing results showed that the targeted drug gene mutations in tissues, arterial CTCs and venous CTCs showed a complementary trend, indicating that there was heterogeneity among different tumor samples. CONCLUSIONS: CTCs in blood can be efficiently captured by the CTCs sorting system based on Vi-LMB/Ep-LMB, and CTCs detection in arterial blood can be utilized to more accurately evaluate the prognosis and predict postoperative progress. It is further confirmed that tumor samples from disparate sources are heterogeneous, providing a reference basis for gene mutation detection before clinical targeted drug treatment, and the detection of CTCs in arterial blood has more potential clinical application value. TRIAL REGISTRATION: The Ethics Committee of Putuo Hospital, PTEC-A-2019-18-1. Registered 24 September 2019.
Authors: Steven A Johnsen; Cenap Güngör; Tanja Prenzel; Sabine Riethdorf; Lutz Riethdorf; Naoko Taniguchi-Ishigaki; Thomas Rau; Baris Tursun; J David Furlow; Guido Sauter; Martin Scheffner; Klaus Pantel; Frank Gannon; Ingolf Bach Journal: Cancer Res Date: 2009-01-01 Impact factor: 12.701
Authors: Andrew A Davis; Qiang Zhang; Lorenzo Gerratana; Ami N Shah; Youbin Zhan; Wenan Qiang; Brian S Finkelman; Lisa Flaum; Amir Behdad; William J Gradishar; Leonidas C Platanias; Massimo Cristofanilli Journal: Breast Cancer Res Date: 2019-12-04 Impact factor: 6.466