Literature DB >> 3413061

A repressor heterodimer binds to a chimeric operator.

M Hollis1, D Valenzuela, D Pioli, R Wharton, M Ptashne.   

Abstract

Replacement of the solvent-exposed residues of the DNA recognition helix of the 434 repressor with the corresponding residues of the P22 repressor generates a hybrid protein, 434R[alpha 3(P22R)], which binds specifically to P22 operators. We show here that a new DNA-binding specificity is generated by combining 434 and 434R[alpha 3(P22R)] repressor monomers to form a heterodimer. The heterodimer specifically recognizes a chimeric P22/434 operator that lacks two-fold rotational symmetry.

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Year:  1988        PMID： 3413061      PMCID： PMC281859          DOI： 10.1073/pnas.85.16.5834

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

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Authors:  D Valenzuela; M Ptashne
Journal:  EMBO J       Date:  1989-12-20       Impact factor: 11.598

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