Literature DB >> 3412552

A microelectrophoretic method for the evaluation of GABA transaminase activity.

A Cupello1, S Larsson, A Palm, M V Rapallino.   

Abstract

The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of [14C]GABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to 14C labeled GABA diffusing across a single microdissected neuronal membrane.

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Year:  1988        PMID： 3412552     DOI： 10.1007/bf00973274

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  5 in total

1.  gamma-Aminobutyric acid stimulates chloride permeability across microdissected Deiters' neuronal membrane.

Authors:  H Hydén; A Cupello; A Palm
Journal:  Brain Res       Date:  1986-07-30       Impact factor: 3.252

2.  Asymmetric diffusion into the postsynaptic neuron: an extremely efficient mechanism for removing excess GABA from synaptic clefts on the Deiters' neurone plasma membrane.

Authors:  H Hyden; A Cupello; A Palm
Journal:  Neurochem Res       Date:  1986-05       Impact factor: 3.996

3.  Protein separation at the cellular level by micro disc electrophoresis.

Authors:  H Hydén; K Bjurstam; B McEwen
Journal:  Anal Biochem       Date:  1966-10       Impact factor: 3.365

4.  Low level tritium and carbon-14 determination.

Authors:  S Larsson
Journal:  Anal Biochem       Date:  1972-11       Impact factor: 3.365

5.  An electrophoretic method for the determination of the proportion of gamma-aminobutyric acid in a mixture of labeled neurotransmitter and its catabolites.

Authors:  A Cupello; M V Rapallino; G Besio; P Mainardi
Journal:  Anal Biochem       Date:  1987-01       Impact factor: 3.365
  5 in total
  2 in total

Review 1.  Unorthodox view of the functioning of a GABAA synapse.

Authors:  Aroldo Cupello; Maria Vittoria Rapallino
Journal:  Cell Mol Neurobiol       Date:  2002-04       Impact factor: 5.046

2.  Can Cl- ions be extruded from a gamma-aminobutyric (GABA)-acceptive nerve cell via GABAA receptors on the plasma membrane cytoplasmic side?

Authors:  A Cupello; A Palm; M V Rapallino; H Hydén
Journal:  Cell Mol Neurobiol       Date:  1991-06       Impact factor: 5.046
  2 in total

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