Literature DB >> 34120716

Optogenetic control of calcium influx in mammalian cells.

Yi-Tsang Lee1, Rui Chen1, Yubin Zhou2, Lian He3.   

Abstract

Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) channel, which has been tailored for optical control of calcium entry and calcium-dependent physiological responses in non-excitable cells and tissues. We describe a detailed protocol for applying Opto-CRAC as an optogenetic tool to achieve photo-tunable control over intracellular calcium signals and calcium-dependent gene expression in mammalian cells.
Copyright © 2021 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Actuators; CRISPR; Calcium signaling; Ion channel; ORAI1; Optogenetics; Protein engineering; STIM1; Synthetic biology; Transcriptional programming

Mesh：

Substances：

Year:  2021        PMID： 34120716      PMCID： PMC8290970          DOI： 10.1016/bs.mie.2021.02.010

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  33 in total

1.  Optogenetic protein clustering and signaling activation in mammalian cells.

Authors:  Lukasz J Bugaj; Atri T Choksi; Colin K Mesuda; Ravi S Kane; David V Schaffer
Journal:  Nat Methods       Date:  2013-02-03       Impact factor: 28.547

Review 2.  CRAC channel-based optogenetics.

Authors:  Nhung Thi Nguyen; Guolin Ma; Eena Lin; Brendan D'Souza; Ji Jing; Lian He; Yun Huang; Yubin Zhou
Journal:  Cell Calcium       Date:  2018-09-03       Impact factor: 6.817

3.  Structure of a flavin-binding plant photoreceptor domain: insights into light-mediated signal transduction.

Authors:  S Crosson; K Moffat
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-27       Impact factor: 11.205

4.  High-performance calcium sensors for imaging activity in neuronal populations and microcompartments.

Authors:  Hod Dana; Yi Sun; Boaz Mohar; Brad K Hulse; Aaron M Kerlin; Jeremy P Hasseman; Getahun Tsegaye; Arthur Tsang; Allan Wong; Ronak Patel; John J Macklin; Yang Chen; Arthur Konnerth; Vivek Jayaraman; Loren L Looger; Eric R Schreiter; Karel Svoboda; Douglas S Kim
Journal:  Nat Methods       Date:  2019-06-17       Impact factor: 28.547

5.  A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Authors:  G Grynkiewicz; M Poenie; R Y Tsien
Journal:  J Biol Chem       Date:  1985-03-25       Impact factor: 5.157

Review 6.  Store-Operated Calcium Entry Mediated by ORAI and STIM.

Authors:  Nhung T Nguyen; Weidong Han; Wen-Ming Cao; Youjun Wang; Shufan Wen; Yun Huang; Minyong Li; Lupei Du; Yubin Zhou
Journal:  Compr Physiol       Date:  2018-06-18       Impact factor: 9.090

7.  Engineering of a bona fide light-operated calcium channel.

Authors:  Lian He; Liuqing Wang; Hongxiang Zeng; Peng Tan; Guolin Ma; Sisi Zheng; Yaxin Li; Lin Sun; Fei Dou; Stefan Siwko; Yun Huang; Youjun Wang; Yubin Zhou
Journal:  Nat Commun       Date:  2021-01-11       Impact factor: 14.919

8.  STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1.

Authors:  Chan Young Park; Paul J Hoover; Franklin M Mullins; Priti Bachhawat; Elizabeth D Covington; Stefan Raunser; Thomas Walz; K Christopher Garcia; Ricardo E Dolmetsch; Richard S Lewis
Journal:  Cell       Date:  2009-02-26       Impact factor: 41.582

9.  Ultrasensitive fluorescent proteins for imaging neuronal activity.

Authors:  Tsai-Wen Chen; Trevor J Wardill; Yi Sun; Stefan R Pulver; Sabine L Renninger; Amy Baohan; Eric R Schreiter; Rex A Kerr; Michael B Orger; Vivek Jayaraman; Loren L Looger; Karel Svoboda; Douglas S Kim
Journal:  Nature       Date:  2013-07-18       Impact factor: 49.962

10.  Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling.

Authors:  Guolin Ma; Lian He; Shuzhong Liu; Jiansheng Xie; Zixian Huang; Ji Jing; Yi-Tsang Lee; Rui Wang; Hesheng Luo; Weidong Han; Yun Huang; Yubin Zhou
Journal:  Nat Commun       Date:  2020-02-25       Impact factor: 14.919
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